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Establishment of Feasible Tissue Culturing Protocol for Artemisia Absinthium L.

Thesis Info

Author

Munawar Ali

Department

Department of Biotechnology, QAU

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2012

Thesis Completion Status

Completed

Page

x, 77

Subject

Biotechnology

Language

English

Other

Call No: DISS/M. Phil. BIO/2870

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676717502150

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حاجی رشید الدین

حاجی رشیدالدین
گذشتہ مہینہ اعظم گڑھ کی ایک پرانی یادگار حاجی رشید الدین صاحب نے بھی وفات پائی، مرحوم ترجمان القرآن مولانا حمیدالدین فراہیؒ کے سگے اور مولانا شبلی مرحوم کے ماموں زاد بھائی تھے، دارالمصنفین سے ان کا تعلق بہت قدیم اور مخلصانہ تھا، اس کی مجلس انتظامیہ کے رکن بھی تھے، مولانا حمید الدین صاحب مرحوم کے بعد مدرستہ الاصلاح سرائے میر کو ان ہی نے سنبھالا تھا، اور عرصہ تک اس کے ناظم رہے، ادھر چند سال سے ضعف پیری کی وجہ سے اس خدمت سے سبکدوش ہوگئے تھے، طبعاً بڑے نیک سیرت، پاک دل اور مرنجاں مرنج تھے، مولانا شبلی کے اعزہ میں سب سے زیادہ سن رسیدہ اب وہی رہ گئے تھے، نوے سال کے قریب عمر تھی، ادھر کئی سال سے بالکل معذور ہوگئے تھے، ہوش و حواس نے بھی جواب دیدیا تھا، صرف رشتۂ حیات باقی تھا، افسوس کہ وہ بھی منقطع ہوگیا، اﷲ تعالیٰ مرحوم کو اپنی رحمتِ کاملہ سے نوازے۔
(شاہ معین الدین ندوی، نومبر ۱۹۵۹ء)

Profil Kitab Tafsir, Model Pemahamannya Pengaruhnya Terhadap Fiqih Ibadah Pada Pondok Pesantren Di Provinsi Riau

Pondok Pesantren (classical Islamic educational institution in Indonesia) uses the classic book strictly and continuously. Students (santri) fanatically follow the book used by the teacher, including in the use of the book of exegesis. Pondok Pesantren in Riau Province uses the book of tafsir differently according to the grouping of the tradition and the teacher's understanding of the Pondokmya. Most of the Pondok Pesantren in Riau Province, using a translation book that is not a yellow book (Arabic Book. This situation tends to eliminate the character of Pondok Pesantren which makes the yellow book (Arabic Book) as the basis and its main characteristic. This paper seeks to see the profile of the book of tafsir, model of understanding and that’s correlation according to relegius jurisprudence on Pondok Pesantren in Riau Province.

Kinetic Study of Biodegradation of Textile Dyestuffs by White Rot Fungi

Potentials of five locally isolated white rot fungi Pleurotus ostreatsus IBL-02, Phanerochaete chrysosporium IBL-03, Coriolus versicolor IBL-04, Ganoderma lucidum IBL-05, and Schizophyllum commune IBL-06, for biodegradation of textile dyes commonly used in Fasalabd texile dyeing process units, were investigated. Dyes (Reactive, Disperse, Direct and Vat) dyes were provided free of cost from Clarient (pvt) limited, Ciba (pvt) limited and Dyestar (pvt) limited. Reactive dyes included Drimarine Blue K2RL, Cibacron blue FG3A, Drimarine Orange KGL, Drimarine Brilliant Red K4BL, Prucion Blue PX5R, and Remazol Brilliant Yellow 3G. The dyes of disperse class were Foron Turquize SBLN-200, Foron Blue RDGLN, Foron Red RDRBLS and Foron Yellow SE4G. Direct dyes group comprised of Solar Golden Yellow R, Solar Brilliant Red BA, Solar Orange RSN and Solar Blue A and Vat dyes included were Cibanon Blue BFMD, Cibanon Golden Yellow RK-MD, Indanthrene Direct Black RBS. White Rot fungi cultures were applied on reactive dyes and a combination of best fungus and best decolorized dye was selected. Reactive Remazole Brilliant Yello3-GL was maximally decolorized by coriolus versicolor in 7 days of incubation and it was processed for further process optimization. Activities of LiP, MnP and laccase were124, 254 and 354 IU/mL were respectively. The C. versicolor strain decolorized 0.01% dye up to 99.6% in 24 hours in Kirk’s medium, I; pH, 4, temperature, 30±20C with the addition of 1% Glucose, 0.1% CSL, 1mM ABTS and CuSO4. Activities of MnP and laccase were 389 and 795 IU/Ml. Adsorption on fungal mycelia was negligible in 0.01 % dye solution but it increased with higher dye the concentration. Disperse dyes were also subjected to decolorization by WRF cultures and maximum decolorization (91.87%) of Foron Turquoise SBLN-200 was caused by Ganoderma lucidumon on 8th day. After optimization of varying media ompositions the dye was effectively decolorized in medium IV which was nitrogen rich and its lignolytic enzyme profile was 282, 115 and 116 IU/mL for LiP, MnP and laccase respectively on 7th day of incubation. Further process of optimization revealed that 99.20% dye was degraded in solution receiving 0.01% dye concentration with production of LiP, MnP nand laccase (636, 531and 382 IU/mL respectiely) under optimum conditions (MediumIV; pH 4.5, temperature, 35±20C, in the presence of 1.5%wheat bran, 0.1% of MGM60%, 1mM Veratryl Alcohol and 1mM MnSO4). Direct dyes like reactive and disperse were subjected to decolorization by WRF and screening experiment proved that Pleurotus ostreatus was efficient in decolorizing Solar Golden Yellow R up to 93.10% in 7 days. After optimization of media composition the dye color removal reached at 98.86% in MediumIII and activities of Lip,MnP, AND laccase were 61,163 and 140 IU/ML respectively. Uner Complete optimum conditions ( at pH 3.5 and temperature 30±20C in presene of 1.0% Wheat Bran, 0.05% of MGM (60%), 1Mm of H2O2 and FeSO4 maximum dye decolorization was achieved in 24 hours of incubation. Activities of major enzymes MnP and Lac were 687 and 376 IU/mL respectively. Dye adsorption was negligible. Vat dyes usually were not soluble in water and dye solution were prepared in dilute NaOH. sluccinic Acid was used to adjust optimum pH. Of all WRF cultures applied on vat dyes, C.versicolor showed maximum decolorization (92.32%) of Cibanon Blue GFJ-MD in 6 days of incubation in Kirk’s basal medium. After media compositions optimization the dye was degraded up to 97.12% in medium II and enzyme activies were 54, 35 and 185 IU/m. After the completion of process optimization medium II ; pH, 4 ; temperature, 30±20C; Glucose, 1%; 1m M ABTS and CuSO4. 0.01% Cibanon Blue GFJ- MD was degraded up to 99.12% in 24 hours. Major enzyme involved in dye degradation was laccase and its was 595 IU/mL. Dye adsorption was 0.06% after 24 hours of incubation which declined with the passage of time, due to degradation of dyes by the enzymes. Addition of nitrogen showed inhibitory effect on fungal enzyme activities and dye removal.