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Separation and Analysis of Metal Ions by Ion Chromatography

Thesis Info

Author

Noshaba Anjum

Department

Deptt. of Chemistry, QAU.

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2005

Thesis Completion Status

Completed

Page

v,65

Subject

Chemistry

Language

English

Other

Call No: DISS/M.Phil CHE/674

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676717820050

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ریت کیسے اُسے الفت کی نبھانی آئے

ریت کیسے اسے الفت کی نبھانی آئے
’’جب سمجھ میں نہ محبت کے معانی آئے‘‘

میں یہ سمجھی تھی کہ تم سے ہے نظامِ ہستی
کیا غضب تم سے ہی اب آنکھ میں پانی آئے

ساتھ چلنے کا ہنر دے کے بچھڑنے والے
چال میں کیسے وہ پہلی سی روانی آئے

دیکھ کے اس کو بہک جائیں فرشتے بھی یہاں
جب کبھی لے کے وہ جھرنوں سی جوانی آئے

وقت کی نبض کو میں خوب سمجھتی ہوں فضاؔ
ذہن و دل میں نہ کوئی یاد پرانی آئے

METODE PARTISIPATIF, CERAMAH, DAN DISKUSI PADA AL QURAN SURAT AS-SHAFFAT AYAT 102

This research is motivated by a phenomenon of poor communication between parents and children. In the world of education, there was a lack of approaches and methods used by educators in providing subject matter so that what was delivered by the educator did not achieve the expected goals. The problem in this research is how the participatory methods, lectures and discussions contained in the As-Shaffat letter verse 102. The purpose is to describe the educational methods contained in the As-Shaffat letter verse 102 in the form of participatory methods, lecture methods and discussion methods. This research is a Library Research. The method of interpretation used in this study is the method of interpretation of maudhu 'or thematic methods which interpret verses of the Qur'an based on specific themes. The method used in the explanation of the verse is the method of tahlili or analysis that explains the verses of the Qur'an by examining its aspects and revealing its whole purpose. The results of study and analysis, as well as data processing from various sources, obtained the first result that the educational method contained in the As-Shaffat verse 102 is a participatory method used by educators, which involves all elements in the educational process especially students, with active participation from participants students will facilitate educators in delivering the material to be given. Both lecture methods, in conveying information, especially learning materials can be done with the lecture method. An educator prioritizes good attitudes so that the material can be accepted by students. The third method of discussion is to involve students in giving their opinions. With the discussion in learning, the lessons will become warmer and will train students to be more courageous in opinion.

Cytogenetic and Molecular Analysis of Chronic Myeloid Leukemia at Hyderabad Sindh.

BACKGROUND: Leukemia is defined as a neoplastic proliferation of hematopoietic tissue of white blood cell precursors in bone marrow CML accounts for 20% of all leukemias in adult patients. One of the characteristic findings in CML subjects is the presence of the Philadelphia (Ph) chromosome and Bcr-Abl oncogene. Philadelphia (Ph) is the shortened chromosome 22, formed by reciprocal translocation between the long arms of chromosomes 9 and 22, t (9; 22). OBJECTIVES OF STUDY: — To determine the frequency of Philadelphia chromosome in CML. — To evaluate the standard and variant translocation in CML. — To determine frequency of other chromosomal abnormalities in Philadelphia +ve CML. — To detect bcr-abl gene rearrangement and to determine the frequency of the b2a2 and b3a2 transcripts in Philadelphia + CML by PCR. SUBJECTS AND METHODS: The study was conducted at the Department of Pathology, Liaquat University of Medical and Health Sciences, Jamshoro and Isra University Hospital, Hyderabad during May-to-September 2014. Bone marrow and peripheral blood samples from a total of 145 diagnosed cases of CML were collected. Cytogenetic analyses were performed using karyotyping as per the international system for human cytogenetic nomenclature guidelines. All karyotpic images were analyzed using the Cytovision software. In order to identify BCR-ABL transcripts, RT-PCR was performed. Statistical analysis of the data was done using SPSS-version-21.0. Results: Of the 145 samples, a total of 133 (91.7%) were positive for the Ph (Ph+) while 12 (8.2%) were negative for the Ph (Ph-). Of the 133 Ph+ samples, standard chromosome was noted in 121 (90.9%), simple variant in 9 (6.7%) and complex variants in 3 (2.2%) of the samples. All the Ph+ samples (n=133) showed BCR-ABL positivity. Of the 12 Ph- samples, a total of 7 (58.3%) were BCR-ABL-positive and 5 (41.6%) were BCR-ABL-negative. Various types of molecular abnormalities were noted in in responders and in non responders as shown in table below. Out of 11 patients, who responded on Glivec, 8 patients showed b3a2 transcripts abnormality while 3 cases had b2a3 abnormality. Both non-responders showed b2a2 + e 19a2 transcripts. CONCLUSION: Philadelphia frequency of 90.9%. Out of 133 Ph +ve all were bcr-abl +ve while out of 12 Ph-ve 7 were bcr-abl +ve while 5 were –ve. Cytogenetic and molecular analysis has become mandatory in order to make a correct diagnosis and predict/monitor response to newer molecular targeted treatment modalities. KEY WORDS: Philadelphia chromosome, BCR-ABL, Chronic Myeloid Leukemia, cytogenetic analyses, Sindhi population.