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Serosurvey of Human Brucellosis in the Rawalpindi Islamabad Area

Thesis Info

Author

Qazilbash Ali Abbas

Department

Deptt. of Biological Sciences, QAU.

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

1996

Thesis Completion Status

Completed

Page

47

Subject

Biological Sciences

Language

English

Other

Call No: DISS/M.Phil BIO/521

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676717887892

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پیش لفظ

شک سے دشت بہیں ، آہ سے دریا سوکھیں
مچھلیاں دشت میں پیدا ہوں ، ہرن پانی میں
کوئی بھی بات کہنے کوبہت چھوٹی یا معمولی ہوتی ہے ، مگر اس بات کا فہم ، ادراک اورسب سے بڑھ کر اس پر تفکر اس کو اعلیٰ اور عظیم بنا دیتا ہے۔ کہنے کو اس دنیائے فانی میں میں کتنے لوگ پیدا ہوئے اور کتنے مر گئے۔ ان میں سے بہت سارے گمنامی کی موت مر جاتے ہیں اور ان کی زندگی کی تلخیوں کے بارے کسی کو خبر تک نہیں ہوتی اور نا ہی ان کے شگفتہ اخلاق لوگوں پر آشکار ہوتے ہیں۔ اس کے بر عکس بہت کم لوگ ایسے ہوتے ہیں، کہہ لیں آٹے میں نمک کے برابر، جو اپنی بات کو منجھے ہوئے انداز میں کرتے ہیں کہ اگلا بندہ ان کی بات سنتے ہی فریفتہ ہو جاتا ہے اور تعریف کرنے پر مجبور ہو جاتا ہے ۔ انگریزی ادب میں ایسی ہی ایک شخصیت سر فرانسیس بیکن ہے، جن کی زندگی دنیا کے تلخ و شیریں تجربات سے گزر کر کندن بن گئی تھی ۔ اپنی زندگی کے تجربات کی روشنی میں انہوں نے بہت سے مضامین لکھے جو کہ حقیقتاً تعریف کے قابل ہیں ۔ یہ کتاب جو آپ کے ہاتھوں میں ہے یہ سر فرانسیس بیکن کے مضامین کا اردو ترجمہ ہے۔ میں نے بارہا اس بارے میں سوچا۔ کچھ مخلص دوستوں کے کہنے پر اس بارے قلم اٹھایا اور بیکن کے کچھ مضامین کو اردو کے قالب میں ڈھال دیا ۔ گو کہ یہ مشکل کام تھا مگر میری زندگی کایہ تجربہ بہت خوش گوار رہا ۔ میں اُس باری تعالی کا بڑا شکر گزار ہوں جس نے مجھے اس معاملے میں قلم اٹھانے کی طاقت دی اور اسے تکمیل بخشی۔

ڈاکٹر محمد قاسم علی رانا
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عورتوں کے حقوق اسلامی تعلیمات کی روشنی میں

Before the advent of Islam, women were living in worst conditions during the Era of Ignorance. Daughters were buried alive. Women were openly sold in the market. A woman was just a source of enjoyment for males and nothing else. She had no basic rights. Even she had no share in inheritance. In human history, it is Islam that for the first time gave due respect to woman by providing her basic rights and proper status. Islam believes in equality of men and women – ‘Equality’ does not mean ‘to become identical’. Women’s rights in Islam pertain to Spiritual, Economic, Educational and Legal Rights. A woman can adopt any profession, she wants. Thus, she has got full freedom to live a happy and peaceful life. Unfortunately, there are many people who believe that because Islam gives men the right of having authority over their wives, therefore, men are superior to women and that men have priority over women in all matters. The fact is that according to Islamic teachings, men are not superior to women; and so women are not superior to men. There is no gender discrimination in Islam. Allah Almighty states very clearly in the Holy Quran that superior in His eyes are only those who have more taqwa (fear of Almighty Allah).

Characterization of Chilli Anthracnose Pathogen and its in Vitro Management

Chilli anthracnose is the most prevalent disease in all major chilli growing areas of the Punjab. Chilli anthracnose is causing the highest yield losses than any other fungal disease. This study was focused to assess the prevalence and incidence of the disease in five major chilli production districts of Punjab. Moreover, morphological and molecular characterization of pathogen and invtiro management by using different techniques of application through five plant extract. Field based surveys were conducted in two years (2014-2015 and 2015-2016) for disease documentation and sample collection followed bylaboratory isolations resulted into the recovery of 142 pathogenic isolates of the pathogen associated with the disease. Disease was recorded in all surveyed area and showed 100% prevalence viz., Vehari, Bahawal Nagar, Okara, Kasur and Rawalpindi. The maximum average incidence 63.10% was recorded at Vehari while minimum average incidence 25.5% was recorded at Rawalpindi.Morphological characterization of the recovered isolates showed variation in morphological characters like two types of colony colours were observed. Fusiform and falcate conidial shapes were recordedand some isolates produced setae while other not. Variations in number of acervuli were also observed. Molecular characterization was done including DNA sequencing of pathogenic isolates though PCR amplification of the Internal Transcription Spacer region using ITS1 and ITS4 primers. Characterization established the identity of the highly pathogenic isolates selected from each district to their morphological characterization done before molecular study. Phylogenetic analysis of 5 morphologically and pathogenically diverse Colletotrichum capsici xxii isolatesexpressed their evolutionary relationship by 99-100% similarity with previously reported isolates available at NCBI database. The most virulent isolate CCO41was used as source of inoculum in in vitro management of the pathogen by using five different plant extracts applied through two different techniques viz., poisoned food technique and well diffusion technique. All applied plant extracts showed antifungal properties and maximum 98.5% growth inhibition was noted down in case of polygonum plant extract @ 0.1% and 0.2% when applied through poisoned food technique while minimum antifungal activity was expressed by dodonaea and clove at the dose of 25μL when applied through well diffusion technique. Poisoned food was found better than well diffusion technique in the study. Results of the study generated exact and comprehensive picture of the disease in major chilli growing areas of the Punjab and indicated the areas at high risk in terms of disease spread. The survey observations also pointed out the trend of decreasing chilli crop area in the all districts due to number of factors including anthracnose. Isolates recovered from the study area were first time studied with details with reference to morphological and molecular characteristics.In vitrobiologicalmanagement studies explored the antifungal potential of indigenous plant against Colletotrichum capsici. Polygonum plant extract was found to be the most effective by suppressing pathogen’s growth up to 98.5% and depicted its antifungal potential.These management findings indicate projected antifungal ability of plant extracts to manage chilli anthracnose in field conditions.