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Role of Quorum Sensing Signal Molecules in Bacterial Physiology

Thesis Info

Author

Sami Ullah Khan

Supervisor

Fauzia Yusuf Hafeez

Department

Department of Biotechnology, QAU

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2008

Thesis Completion Status

Completed

Page

90 +Aendices

Subject

Biotechnology

Language

English

Other

Call No: DISS / M.PHIL / BIO / 3374

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676718430302

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علامہ محمد بن صالح العثیمین

علامہ محمد بن صالح العثیمین
پچھلی صدی عیسوی کا اختتام عالم اسلام کی متعدد مایہ ناز شخصیات کی المناک وفات پر ہوا تھا، علامہ عبدالعزیز بن باز، مولانا سید ابوالحسن علی ندوی، شیخ ناصرالدین البانی اور شیخ محمد عمر فلاتہ جیسے آسمان علم و فضل کے آفتاب و ماہتاب اس کے کہر میں چھپ گئے تھے۔ اب اس صدی کا آغاز بھی ایک متواضع، منکسر المزاج اور درویش عالم کی اندوہ ناک وفات سے ہوا، جو علمی حلقوں میں ابن عیثمین کے نام سے مشہور و متعارف تھے۔
مرحوم سعودی عرب کے ایک قدیم معزز خاندان کے چشم و چراغ تھے، صوبہ قصیم کے شہر عنیزہ میں ان کی ولادت ہوئی، شیخ عبدالرحمن السعدی اور مشہور مفسر شیخ محمد امین شنقیطی سے اکتساب فیض کیا، شیخ السعدی ان کے ہم وطن اور ان کے ابتدائی استاد تھے اس لیے وہ ان سے زیادہ متاثر ہوئے اور ان کے انتقال کے بعد ان کے علمی جانشین مقرر ہوئے۔
مدۃالعمر عنیزہ میں تدریس، وعظ و ارشاد اور تصنیفِ رسائل میں مشغول و منہمک رہے، سعودی عرب میں شیخ ابن باز کے انتقال کے بعد علماء اور طلبہ علوم دینیہ کا مرکز وہی بن گئے تھے مگر شیخ ابن باز کی جدائی ان کو گوارا نہ ہوئی، ان کی وفات کو ابھی دو برس بھی نہیں گزرے تھے کہ شیخ عثیمین نے بھی رختِ سفر باندھا اور ان سے افادہ و استفادہ کا سلسلہ موقوف ہوگیا۔
جدہ کے ایک اسپتال میں ان کا انتقال ہوا، مکہ مکرمہ میں ان کی نماز جنازہ پڑھی گئی اور مقبرہ العدل میں شیخ ابن باز کے پہلو میں مدفون ہوئے۔ تقریباً پانچ لاکھ نفوس نے ان کے جنازہ کی مشایعت کی جن میں سربراہان مملکت بھی شامل تھے جو ان کی مقبولیت کا بین ثبوت ہے۔
علامہ مرحوم بڑے خلیق، متواضع اور سادگی پسند...

تفسیر الدر المنثور فی التفسیر با لماثور میں بعض موضوعی روایات کا علمی اور تحقیقی جائزہ: سورۃ النساء تا سورۃ المائدہ

Jalal-ud-Din Abdur  Al Rahman ibn Bakr-Suyuti who has written a well-known Tafseer Al-Durr Al-Man’thur  fi al-tafsir Bil-Ma’thur. This is a big treasurer of explanatory traditions but unfortunately he has quoted some fabricated narrations in this tafseer which caused doubt about the validity of his commentary. This article deals with the some fabricated report.

Morphological and Biochemical Characterization of Tomato Accessions and Their Screening Against Major Viral Diseases under Controlled Environment

A set of fourty tomato accessions having diverse origin were tested at two locations viz., Swabi and Haripur Districts, Khyber Pakhtunkhawa, Pakistan, to assess morphological and biochemical attributes viz-a-viz resistance level for major viral diseases. The experiments were carried out in Randomized Complete Block Design (RCBD), with three replications. Data was recorded on morphological traits across two locations, during 2013-14. Biochemical analysis was carried out during 2013-14 in central laboratory, University of Haripur. Screening against Tomato Mosaic Virus (ToMV) and Tomato Yellow Leaf Curl Virus (TYLCV) were carried out at the Department of Agro-ecology Flakebjerg Research Institute of Aarhus University, Denmark. DAS-ELISA test and TAS-ELISA test (at different leaf stages) were performed in the laboratory for screening the samples against ToMV and TYLCV, respectively. However, disease symptoms were recorded in glass house under controlled environment on inoculated plants. Highly significant differences were oserved between locations except fruits weight plant-1, which manifested non-significant differences. Similarly, accessions (G) and G × E interaction for majority of the traits revealed highly significant differences except plant stem diameter, plant stem girth, fruit width, locules fruit-1, corolla color, fruit shoulder shape, plant growth type and predominant fruit shape. For all the biochemical attributes except fats, accessions revealed significant differences. Oveall accessions showed best performance in the environmental conditions of Haripur than Swabi for yield and yield attributing traits. The accessions in question exhibited extraordinary performance for biochemical traits as well. Regarding genotypic coefficient of variation (GCV), high estimates (21.79 to 86.38) were recorded for majority of the traits. Furthermore, phenotypic coefficients of variation (PCV) values were moderate to high (13.00 to 90.66) for all the traits except days to first flowering in both locations. Similarly, all biochemical traits revealed moderate to high GCV and PCV except moisture content, pH, oxidation reduction potential and percent protein. High broad sense heritability estimates were observed for majority of the morphological traits in Swabi (0.62 to 0.99) and Haripur (0.61 to 0.99). High heritability ranges were 0.69 to 0.98 for all biochemical traits except moderate heritability for protein (0.39%). In Swabi, high selection response was observed for fruits plant-1 (99.37) and plant height (50.38 cm). However, yield plot-1 revealed moderate selection response (10.09). Similarly, high genetic advance was observed for fruits plant-1 (79.53) and moderate for clusters plant-1 (9.67) and plant height (16.78 cm) in Haripur. Furthermore, moderate selection response was observed for potassium (11.8042 mg/100g) and oxidation reduction potential (15.12 mV). Yield components revealed highly significant positive correlation with yield hectare-1 and yield plot-1. Calcium revealed significant positive correlation with potassium, oxidation reduction potential and fiber content. Sodium exhibited highly significant positive correlation with potassium. Total sugars manifested highly significant correlation with reducing xi sugars and non-reducing sugar. Total soluble solids indicated highly significant positive correlation with pH, reducing sugars and total sugars. Highly significant positive association was observed for firmness with total sugars and total soluble solids. Vitamin-C showed highly significant positive correlation with moisture content. Principal component analysis was carried out based on 27 morphological traits. It was found that 5 out of 27 principal components with an Eigen value above than 1.0 considered for 74.10% of the total variation. Five principle components viz., PC-1 (37.12%), PC-2 (15.79%), PC-3 (8.17%), PC-4 (9.94%) and PC-5 (6.07%) were more contributing to total variation. In PC-1 accessions (Acc-10572, Acc-17867, Acc-17872, Acc-17889 and CLN-362) and accession STM-2 in PC-6 showed greater genetic variability. Principal component analysis for 18 biochemical attributes of tomato accessions revealed that Eigen value for 5 out of 18 principal components ranged between 1.00 to 59.99%. Five principal components viz., PC-1 (17.23%), PC-2 (31.20%), PC-3 (52.07%), PC-4 (42.16%) and PC-5 (59.99%) were found more participatory in the total variation. First and second principal components showed that the accessions were dispersed in all quarters, which revealed high extent of genetic variability. It was observed in the PC-1 accessions AVR-201, CLN-282 and in PC-2 Acc-17890 showed greater genetic variability. On the basis of morphological and biochemical traits all the accessions were divided into four and five cluters, respectively. These clusters were further divided into subclusters. DAS-ELISA test against Tomatoes Mosaic Virus (ToMV) revealed significant differences in overall mean values. Among all the accessions, the accession Acc-17878 was found resistant to Tomato Mosaic Virus. However, on the basis of TAS-ELISA test and disease symptoms severity scale for TYLCV, accessions Acc-17890, AVR-261, CLN-312, AVR-321, EUR-333, CLN-352 and CLN-362 revealed negative sign with no symptoms. Based on morphological and biochemical characterization, heritability, selection response and G × E interaction, the attributes viz., earliness, fruits plant-1, yield plot-1, clusters plant-1, fruits cluster-1, potassium, lycopene, oxidation reduction potential, firmness and fiber content the accessios CLN-312, Acc-17889, AVR-241, Acc-17878, CLN-352, AVR-211, CLN-282, EUR-303, Acc-10587 showed best performance. High estimates of heritability and selection response suggest that genetic variance could play an important role in the inheritance and selection for improvement of these attributes. Therefore, these tomato genotypes could further be explored in future breeding program for varietal development. For disease resistance viz., tomato mosaic virus, the accession Acc-17878 and for yellow leaf curl virus, the genotypes Acc-17890, AVR-261, CLN-312, AVR-321, EUR-333, CLN-352 and CLN-362 confirmed resistance. It is therefore, recommended that in future tomato breeding programes the disease resistance characters may be incorporated into high yielding and quality attributed accessions through back crossing.