عورت اور مصری تہذیب
قدیم مصری تہذیب میں اکثر و بیشتر بادشاہ اپنی بہن سے شادی کرتا حتیٰ کہ بیٹی سے بھی شادی رچائی جاتی تھی ۔اس کے لیے تاویل یہ پیش کی جاتی کہ شاہی خون خالص رہے۔ فرعونی دور کی تحریروں کو جب ڈی کوڈ کیا گیا تو معلوم ہو ا کہ مصری شاعری میں لفظ بھائی بہن محبوب اور محبوبہ کے معنوںمیں بھی استعمال ہوتا تھا ۔بادشاہوں کے حرموں میں بہنوں کے علاوہ سینکڑوں کنیزیں رکھنے کا شوق اپنی جگہ مگر متوسط آمدنی والے مصر کے عام لوگ یک زوجگی پر قانع رہتے تھے ۔خانگی زندگی بدیہی طور پر بڑی حد تک بہتر تھی۔عورت کو طلاق دینا آسان نہ تھا ۔عقد میں آنے والی عورت کو جائیداد میں اچھا خاصا حصہ ملتا۔ایک مغربی مفکر کا قول ہے کہ کسی بھی قدیم یا جدید تہذیب نے عورت کو وہ بلند قانونی رتبہ نہیں دیا جتنا وادی ِ نیل کے باشندوں نے دیا۔ اپنی تند خو (سقراطی) بیویوں کو گھر میں بند رکھنے کے عادی یونانی سیاح یہ آزادی دیکھ کر ششدر رہ جاتے ۔ فرعونی دور کے ادب میں عورت کی حیثیت اور عظمت کے گُن گائے جاتے تھے۔ مصری عورت سے محبت ایک قومی فریضہ سمجھا جاتا تھا۔ مصری مرد کو صرف مصری عورت سے ہی قلبی اور جنسی وابستگی کی ترغیب دی جاتی۔ ایک مصری بزرگ اپنے سننے والوں کو سمجھاتے ہیں کہ’’ باہر سے آنے والی ایسی عورتوں سے ہوشیار رہو ۔یہ گہرے پانیوں کے بھنور کی مانند ہوتی ہیں‘‘۔اسی طرح ایک مصری اپنے بیٹے کو نصیحت کرتے ہوئے لکھتا ہے کہ’’اگر تم نے اپنا گھر کامیابی کے ساتھ سجا سنوار لیا ہے اور خوب صورت ترین بیوی تمھاری آغوش میں ہے تو اس کا پیٹ بھرو اور کمر پر کپڑا ڈالو۔اس کی خوشی کا سامان مہیا کرو کیوں...
Background and Aims: Muscle strength is the key area to measure the functional status of an individual. Different tools and techniques has been used to detect strength differences and deficits. Hand- held dynamometer is one of the most affordable and handy tools used for this purpose. This study was designed to determine intra-rater reliability of hand- held dynamometer to measure muscle strength in different muscle groups of lower extremity of young athletes. It will further explore the reliability of hand- held dynamometer.
Methodology: In this cross- sectional study young players of squash and badminton in the age group of 18-26 years were selected. The participants were recruited by non- probability convenience sampling technique. The strength of major muscle groups of lower limb was measured by a single male tester twice with gap through isometric make test of dynamometer. The intra-class correlation coefficient was then calculated for two readings of each muscle group by using SPSS version 21.
Results: The intra- class correlation coefficient showed good to excellent reliability. The hip abductors, hip adductors, hip extensors of left side, knee flexors and knee extensors showed excellent reliability. Whereas, hip flexors, ankle plantar- flexors and dorsi-flexors of both sides showed excellent reliability at 95 % confidence interval.
Conclusion: The isometric make test of dynamometer is a reliable tool for the objectification of strength of lower limb in young players participating in squash and badminton.
Microbial enzymes such as xylanases enable new technologies for industrial processes. Xylanolytic enzymes hydrolyze complex polysaccharides like xylan and have various commercial applications such as those in the biofuel and pulp and paper fields. In this study, the xylanases from Bacillus pumilus strain BS131 and Aspergillus flavus strain ZGCL17 were isolated from rhizosphere of agricultural fields of district Lahore, Pakistan. These microbes were used as source for production of xylanase and ethanol. A reference strain Aspergillus niger AB1.18 was used as positive reference provided by the courtesy of TNO Netherlands. In the first phase of study, several bacterial and fungal isolates were obtained and purified by subsequent sub-culturing. These purified microbes were then grown on xylan screening media and two strains, BS131 and ZGCL17 were selected because of their significant production capacity. Strain BS131 produced maximum xylanase enzyme (388.8 IU/mg) in xylan medium under submerged fermentation whereas, strain ZGCL17 showed maximum xylanase activity of 493.3 IU/mg in the same medium. In the second phase of study, potential of waste fiber sludge was evaluated to be used as substrate for xylanase production. Waste fiber sludge (WFS) and wheat bran (WB) were used as carbon sources either independently or in combination. Waste fiber sludge was supplemented with different concentrations of salts as well. Along with these salts some combinations were containing wheat bran as an additional Carbon source. Maximum xylanase production was observed in Vogel‟s medium supplemented with WFS as main source of carbon with highest significant xylanase production by strain BS131 (5.7 IU/mL) and strain ZGCL17 (7.3 IU/mL). Along with that, various physio-chemical parameters affecting xylanase production (different media, effect of consortia, incubation period, temperature, pH, substrate concentration, Carbon and Nitrogen sources and enzymatic hydrolysis) were optimized to get maximum enzyme production. Ethanol production was also observed by double fermentation process, involving waste fiber sludge as fermentation substrate. In the first phase of fermentation, Saccharomyces cerevisiae was used for the production of ethanol. In second phase two previously selected microbes (BS131 and ZGCL17) were grown on the resultant product of first fermentation step, to observe xylanase production. Quantification analysis provided that xylanase production improved approximately up to 45% in two step fermentation. These observations strongly indicate the suitability of this two step production system of xylanase enzyme. In the next phase of study, microbial xylanases were purified for chemical characterization and structure elucidation. Column chromatography was used to purify xylanase from rest of the entities. Purity of the purified xylanases was confirmed by performing SDS-PAGE analysis. Purified xylanase fractions were sent for MALDI-TOF/MS analysis. Obtained amino acid patterns were compared by previously deposited sequences on NCBI Protein Blast. All the obtained sequences showed resemblance with xylanase enzymes. The purified xylanase produced by Bacillus pumilus strain BS131 was recognized as glucuronoxylanase with a 100% resemblance with the Bacillus pumilus glucuronoxylanase (EDW23359.1). Whereas, the xylanase data obtained for Aspergillus flavus strain ZGCL17 showed 100% homogeneity with the endo-1,4-beta-xylanase Aspergillus flavus NRRL3357 (XP_002380174.1). In the last phase of study, various physio-chemical parameters were optimized to ensure maximum enzyme activity on previously selected substrate combinations. Among different parameters studied (temperature, pH, substrate concentration, Carbon & Nitrogen sources etc.) maximum xylanase activity was observed at neutral pH in case of BS131 and at pH 5 for ZGCL17. The most suitable temperature for xylanase production was estimated to be 30 °C. With respect to substrate specificity studies 4% of WFS:WB was observed to perform on best significant places. In the same way, some kinetics related to temperature and pH stability was also optimized to ensure maximum enzyme activity. Temperature ranged from 30-60 °C and pH values in same regard were 5.0-7.5. All the experiments were performed thrice containing five replicates of each treatment. All the data was analyzed statistically by performing ANOVA and DNMRT with the help of computer aided software DSAASTAT. Based on the results obtained from the present study it is concluded that two rizospheric microbial strains BS131 (Bacillus pumilus) and ZGCL17 (Aspergillus flavus) have the potential for industrial scale production of xylanase enzyme. Along with that WFS and WB have potential for industrial production of xylanase enzyme. The stability of enzyme was observed over a wide range of temperature. Other characteristics observed add further evidence to its potential for industrial applications. The results of present study strongly indicate the scope for further research on some biochemical structural elucidation and enzyme engineering for wide range of applications.