ڈاکٹر عبدالستار صدیقی
افسوس ہے کہ ہماری علمی بزم کی ایک اہم یادگار ڈاکٹر عبدالستار صدیقی نے گزشتہ مہینے انتقال کیا، وہ اس دور کے مشہور فاضل اور نامور محقق تھے، ان کا موضوع عربی لسانیات تھا، اس کے متعلقات علم الاشتقاق، رسم الحظ حروف و اصوات وغیرہ پر ان کی نظر بڑی دقیق تھی اور اپنی تحریروں میں اس کا بڑا اہتمام رکھتے تھے، ان کی پوری زندگی علمی و تعلیمی خدمات میں گزری، مگر لکھتے کم تھے، انھوں نے غالباً متفرق مضامین کے علاوہ کوئی مستقل تصنیف یادگار نہیں چھوڑی، مگر ان کے یہ مضامین ان کی محققانہ نظر کا ثبوت ہیں، عرصہ ہوا الٰہ آباد ہونیورسٹی کے شعبۂ عربی و فارسی کی صدارت سے ریٹائر ہوئے تھے اور الٰہ آبادی ہی میں سکونت اختیار کرلی تھی، ایک زمانہ میں ہندوستانی اکیڈمی الٰہ آباد کے رکن رکین تھے، یونیورسٹیوں اور دوسری علمی مجالس میں ان کا بڑا وقار تھا، دارالمصنفین کی مجلس انتظامیہ کے بھی رکن تھے، ان کا رہن سہن تو جدید تھا، لیکن شرافت و وضعداری اور شفقت و محبت میں مشرق تہذیب کا نمونہ تھے، ادھر کئی سال سے بالکل معذور ہوگئے تھے، آخر میں ہوش و حواس نے بھی جواب دے دیا تھا، اسی حالت میں گزشتہ جولائی میں انتقال کیا، انتقال کے وقت ۸۷ سال کی عمر تھی، مسلمانوں میں ایسے محقق اب مشکل سے پیدا ہوں گے، اﷲ تعالیٰ ان کی مغفرت فرمائے۔ (شاہ معین الدین ندوی،اگست ۱۹۷۲ء)
Liver plays an important role in lipid metabolism and any acute or chronic malfunction of the liver due to viral hepatitis or liver cirrhosis may induce lipid derangements Objective: To determine the patterns of deranged lipid profiles in patients of chronic hepatitis C Methods: It is a prospective, observational study, conducted at Medicine Department, Mayo Hospital Lahore for 6 months i.e.1stJanuary to 30th June 2018. After the ethical approval, 160 diagnosed cases of chronic hepatitis C of ages 18-70 years of either gender were selected by non-probability purposive sampling. Informed written consent was taken. Demographic information such as name, age and gender were recorded. Venous blood samples from patients after 10 to 14 hours of fasting were drawn for lipid profiles and sent to pathology laboratory. All results were expressed as mg/dl. Data were analyzed in SPSS version 22 Results: Out of 160 patients in this study, there were 94 males and 66 females. Total cholesterol was lower in 62.5% patients, normal in 33.75% patients and higher in 3.75% patients. Triglycerides levels were low in 66.25%, normal in 33.125%, and high in 0.625% patients. Low density lipoprotein (LDL) levels were low in 82.5% patients, normal in 10% and raised in 7.5% patients. High density lipoprotein (HDL) levels were low in 95%, normal in 4.375% & high in 0.625% patients Conclusions: Low levels of serum lipids including total cholesterol, triglycerides, LDL and HDL are seen in population suffering from chronic HCV infection.
Roses have contributed to our social, economic and religious life hence they are called “Index of Civilization”. Presently in Pakistan most of the rose cultivars have been introduced from other countries hence they fail to fit in new environmental conditions of the country. Keeping in view the importance of this issue present study was aimed to get insight into the breeding procedure, complexities and tactics leading to achieve hybrid progenies and to study the magnitude of hybrid vigor inherited in the next generation of parent roses. For this purpose, comparatively well acclimatized and hip producing cultivars including “Autumn Sunset”, “Iceberg”, “Paradise”, “Angel Face”, “Casino”, “Louise Odier”, “Grand Margina”, “Handel” and “Gruss an Teplitz” were selected for crossing. They were planted in the Rose field of Institute of Horticultural Sciences, university of Agriculture, Faisalabad, Pakistan. Morphological description of all cultivars revealed various blends of color of flowers, foliage and leaf hairiness, petiole pubescence and inflorescence types. Data regarding flowering and yields traits also varied in all varieties, however over all better performance was exhibited by the cultivars Iceberg and Gruss an Teplitz scoring 2.5 points each. Poor performance was exhibited by the cultivar Louise Odier. The genotypic and phenotypic and environmental coefficients of variations varied for all quantitative traits. Strong positive correlation was exhibited between number of flowers per plant and overall performance of bushes, flower diameter and petal length, bush shape and height of the plant however fragrance was negatively correlated to flower persistence life. Cluster analysis of the parent on the basis of quantitative traits revealed four major clusters which predicted the different performance for some traits but same for other. Within the individual cluster all the cultivars almost behaved similar. Results about pollen vigor parameters indicated that great variations in pollen quality, quantity, and viability and germination % ages. Maximum number of pollens per anther were exhibited by the cultivar Handel (1612.3) followed by cultivar Angel face (1588.7). Minimum numbers of pollens per anther were counted in the cultivar Iceberg. On average basis the performance of Angel face and Handel was better with respect to both number of anther per flower and number of pollens per anther. Pollen size (L: D) was almost same in all cultivars hence no significant differences were observed. Comparison of means of viability %age revealed that maximum number of viable pollens in term of viability %age were possessed by cultivar Handel (70%) followed by ‘Gruss an Teplitz’ (64%). The minimum viability of pollens was exhibited by the Cultivar ‘Autumn Sunset’ having pollen viability of 35%. Germination medium having 15% sucrose proved very effective for optimum germination results as compared to 10% and 20% sucrose. On average basis the cultivar ‘Grand Margina’ yielded maximum pollen tube length of 46.556 μm followed by ‘Gruss an Teplitz’ having pollen germination of 40.333 μm. The minimum pollen tube length of 9.222 μm was observed in cultivar ‘Iceberg’. Cluster analysis of cultivars on the basis of pollen quality and vigor parameters revealed three main groups. A strong correlation among pollen germination %age and pollen tube length (r = 0.77) and pollen germination %age and pollen viability %age (r = 0.731) was exhibited. Pollen germination is also strongly correlated with pollen size (r =0.738) The comparison of various fertility parameters in vitro and in vivo revealed that cultivar “Handel” showing its maximum pollen viability in vitro performed well with respect to giving more crossing success in field as compared to its average pollen germination %age invitro conditions followed by the cultivar Autumn Sunset. Cultivar Ice Iceberg did not yielded any successful cross. Maximum hip setting %age (83) was observed in for the cross combinations Iceberg x Gruss an Teplitz and Iceberg x Casino and minimum (30%) in Louise Odier x Paradise. Number of seed per hip also varied from 33 to 15 in all cross combinations. Response of seeds with respect to germination also varied significantly after treating the seed with various storage temperatures and acid treatments. Results indicated that germination period (F= 89.95, F<0.05), germination %age (F=67.16, P<0.05), imbibition period (F=26.13, P<0.05) and seed vigor index (F= 112.2, P<0.05) was affected significantly by all treatments accordingly. All the treatments also affected morphological traits of seedlings as well. Evaluation of hybrida in the field revealed that all the F1 hybrids produced showed significant variations in qualitative and quantitative traits over their parents. Data regarding heterosis and heterobeltiosis %ages showed also negative and positive results for various traits over their parents. Variations were observed for flower color and diameter, prickles, bush shape and overall performance of all F1 hybrid progenies. Performance of F1 progenies was evaluated in the field conditions while having comparison with their respective parents and data was analyzed by ANOVA to check the significance of variations in the parents and progenies and comparison of means. Further, more hybrid vigor estimation (Heterosis and Heterobeltiosis) proved a useful tool to check the superiority and inferiority of progenies over their parents. Cluster analysis of the parent cultivars and progenies was useful to develop realation on the basis of similarity index among parent and hybrid progenies. Hybrid identification and genetic diversity studies among parents and progenies evaluated by SSR markers also confirmed the contribution of both parents for the development of F1 hybrids. For genetic diversity study SSR loci proved very effective to amplify across parent cultivars, hybrids and to assess their polymorphic contents. Ten of the SSR primers exhibited similar sequence with known gene. Estimates of genetic diversity varied remarkably among loci. Observed heterozygosity (Ho) based on Nei’s estimation revealed that the locus H10, CL9 and RW54 contributed equally for generating more variation and scored maximum number of alleles for respective locus. Allele fixation trends were also observed for different loci and individual groups. Total gene diversity (Ht) was calculated and mean for all loci was 0.852 while the average gene diversity (Hs) for all loci was calculated as 0.768. It was clear from the dendrogram that parent cultivars compiled themselves into four main groups. Little differences were observed within the groups of parent cultivars. Confirmation of hybrids was done by comparing the common alleles between parent and hybrid against all primers. More over phenatic relationship was developed among hybrid progenies and parents to confirm the extent of contribution of each parent for genetic makeup of hybrid. All other hybrids were identified in the same method. It is concluded from the present study that hybrid cultivars have varying level of fertility and it is necessary to evaluated them in search of vigorous pollen donor parent. It is concluded from the result that diallel was not possible as most of the crosses failed. However ANOVA, comparison of means and Cluster analysis aided by SSR fulfilled the objective. Hybrid progenies exhibited vigor over parents for some traits but for other traits they remained uneffective in the climate of Faisalabad city. SSR markers proved helpful tool for confirmation of F1 hybrids and discriminating the parent cultivars and progenies.