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Isolation and Production of Antimicrobial Compounds by Indigenous Fungal Strains

Thesis Info

Author

Syeda Besma Sabir

Supervisor

Samiullah Khan

Department

Department of Microbiology, QAU

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2016

Thesis Completion Status

Completed

Page

vii, 78

Subject

Microbiology

Language

English

Other

Call No: DISS / M.PHIL / BIO/ 4318

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676718999550

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لبوں کی لالی بتا رہی ہے

ہجر اثاثہ رہ جائے گا
درد خلاصہ رہ جائے گا

آدم زاد سے لغزش ہو گی
ہاتھ میں کاسہ رہ جائے گا

زخم تمھارے ہیرے موتی
درد ذرا سا رہ جائے گا

شعر نگر میں نام ہمارا
اچھا خاصا رہ جائے گا

سب کی پیاس بجھانے والا
دریا پیاسا رہ جائے گا

آج تمھارے ساتھ فضاؔ بس
جھوٹ دلاسا رہ جائے گا

دور جاہلیت میں عرب کے معاشی حالات کا ایک جائزہ

It would be essential to know the pre Islamic economic conditions first, then it would be better to know that what are the Islamic principles regarding trade and economics. This would make us understand the Islamic principles fully; also it would help us to comprehend the nature of Arabs who were the same people who after coming into the fold of Islam agreed to lay down their lives for the Islamic cause. Keeping this in view, we can precise how much Islam advocates the truth and dignity for individual and business. Pre Islamic era was a dark period. Arabs had limited sources of Income and they relied upon the piracy and loot and plunder. In Dark era poets boasted Arabs loot and glorified it in their poetries. No one can deny that Arabs had bravery in their blood, and they conceive courage from their mother's womb. They used to loot money from affluent in the day time and gave away to the needy who used to boast them in their poetries. This Article discusses the Arab’s economic conditions in pre-Islamic Era.

Seroepidemiology Zoonotic Potential & Chemotherapy of Neosporosis in Dogs & Cattle.

In Pakistan, Livestock is considered as a social security for poor villager as it can be a source of cash at the time of need. Degnala disease reduces the production of these animals directly. Along with other side issues related to Degnala disease, this study was done to diagnose the actual cause of Degnala disease by applying different latest scientific techniques. Prevalence along with risk factors was calculated in the rice growing areas of Punjab, Pakistan. Fungal isolation (n=40) was performed from the rice straw feedings of the Degnala disease affected animals through the technique of spot culture on SDA. Then these fungal isolates were identified through comparing their microscopic and macroscopic characters. Then toxigenic potential was checked for all these isolates through the application of TLC and HPLC. After that, from those isolates which were positive for mycotoxin production potential, most cytotoxic isolate was checked with the application of MTT assay. Then the most cytotoxic isolate was inoculated on non-contaminated rice straw and fed to the experimental animals to see a similarity of natural cases of Degnala disease. Finally treatment was conducted to see a proper combination of various drugs against this disease. Toxigenic potential of different candidate fungi, isolated from rice straw feeding of Degnala disease affected bovines was analysed along with Species, age, gender and season wise prevalence. Out of 1536, 104 (6.77%) cases showed positive signs for this disease with a significant association (p<0.05) between rice straw feeding in buffaloes, winter season and bovines having an age of more than one year. Complete Blood Count showed marked increase in erythrocyte sedimentation rate and all white blood cells numbers, except lymphocytes in positive cases. There was a significant increase (p<0.05) in Alanine amino transferase, Aspartate amino transferase and Alkaline phosphatase noticed in Liver Function Test. At the same time, increased value of Creatinine was noticed in Renal Function Test. For isolation and screening of toxigenic fungi, rice straw samples (n=40) being fed to the positive cases were processed further, out of which there were 85 fungal isolates mainly of Aspergillus (57), Penicillium (10), Fusarium (04), Zygomycetes (03), Curvularia (01) and unidentified (10). All isolated fungi were subjected for mycotoxin production and only 11 showed mycotoxin producing capability (including Aspergillus, Penicillium and Fusarium isolates) analysed by Thin Layer Chromatography and quantified through High Performance Liquid Chromatography. It is concluded that all the fungi, contaminating rice straw feeding of Degnala affected animals are not toxigenic. This work will help in establishing major mycotoxin producing fungi leading to the probable cause of Degnala disease in bovine. With the help of MTT assay on vero cell line, most cytotoxic fungus was identified. After an incubation with vero cells, OD values of all the candidate fungi were compared through one way ANOVA. Results of this analysis showed that Fusarium was at the highest ranking and then was the A. flavus with a significant value of 0.006 and 0.039. Finally it was concluded through these systematic steps of converging the diagnosis that, out of all the 85 suspected fungi, Fusarium (isolate number S 8.1) was the most cytotoxic isolate obtained from the rice straw feedings of Degnala affected animals in our study. For molecular diagnosis of the most cytotoxic isolate of Fusarium, PCR was conducted and the results showed that ultimately the final PCR product was successfully amplified against the mentioned primer of ITS conserved region for Fusarium genera and the DNA product was with a length of 570 base pairs. Experimental feeding trials were conducted by inoculating Fusarium (the most cytotoxic isolate) and A. flavus (second most cytotoxic one after Fusarium) separately and in combination compared with the negative control group, all groups were of eight animals each. It was concluded that alone Fusarium was able to produce Degnala disease, while its combination with A. flavus was more lethal. Ultimately the treatment trials proceeded with penta-sulphate, oxytetracycline and antiseptic topical application as therapeutic treatment were shown to be very effective against Degnala cases. While in all the affected animals feeding of affected rice straw was ceased. Only withdrawal of affected rice straw from the feedings of Degnala affected animals was not effective unless proper treatment as mentioned here was not conducted. Analysed. The expected results of the study shall be helpful to make exact diagnosis and treatment of infected buffaloes and cattle that is further helpful for timely prophylaxis and control of the Degnala disease in the rice growing areas of Pakistan and South Asia.