عبادات(نماز و زکاۃ): قرآنی سیاق و سباق کے تناظر میں ذہنی سکون کا منبع

This is the well-known fact that ebadat are the most important articles of Islam. Ebadat bring many spiritual and material benefits to worshipers (Muslim), and this included peace of mind and satisfaction of heart. This point is discussed in this article in detail with reference to the relevant verses of Quran and Prophetic Sunnah in the light of Tafaseer perspectives. A person who bow to Allah Almighty sincerely, he offers prayers in time and pay Zakat to get the Will of Allah, he becomes a great man who is blessed with peace of mind as being agree in every condition with believing in Allah SWT being as satisfaction for him and makes him free from mental tension and anxiety. In the view of Quran e Kareem, the main reason for giving details of the rewards and benedictions of the Paradise is to develop satisfaction within the hearts of the worshipers. It is observed that only the way of attaining real peace of mind and satisfaction of heart is to be punctual and regular in offering prayers and paying zakat sincerely realization in the true sense.

Molecular Detection and Charaterization of Escherichia Coli Isolated from Raw Cow Milk Samples Collected from District Muzaffargarh

Milk is generally considered as highly nutritious and useful food for all age groups as well as inexpensive and widely available. However, its quality and microbial content are the features which have to be observed. The quality of milk depends on its biochemical composition and hygienic conditions during the collection of milk and its distribution. Milk itself acts as best medium for enhancing the growth and development of different types of microbes because of its composition and presence of water in large quantity. Milk sold in Muzaffargarh district was suspected to be contaminated because of repeated outbreaks of gastroenteritis. In this context, the research was designed to rule out molecular detection and quantitative analysis of Escherichia coli (E. coli) in the milk samples obtained from various tehsils of Muzaffargarh district. E. coli were considered as the most prevalent bacteria of milk and could be the most probable microbe causing this disease. In this research, 100 raw milk samples were gathered in a way that 10 samples each were collected from Jutoi, Alipur, Kotadu, Murad Abad, Choak Qureshi, Ahmadpur, Rohilan Wali, Shahjamal, Khanpur and Muzaffargarh city. Then the samples of milk were cultured on different culture media for bacterial segregation. Identification of bacterial specie was done through gram?s staining and properties of bacterial culture on different selective media. Biochemical tests were also performed which include catalase and coagulase tests. Final identification was performed through PCR and resolution of PCR products by gel electrophoresis. Antibiotic sensitivity test was also performed so as to confirm the susceptibility of E. coli regarding multiple antibiotics. Out of 10 samples collected each from Jutoi, Alipur, Kotadu, Murad Abad, Choak Qureshi, Ahmadpur, Rohilan Wali, Shahjamal, Khanpur and Muzaffargarh city, results had shown the presence of E.coli in 4(40%), 6(60%), 3(30%), 2(20%), 4(40%), 5(50%), 3(30%), 4(40%), 6(60%) and 5(50%) samples respectively. E. coli isolates were amplified by PCR based on 16S rRNA gene. Results of antibiotic sensitivity test revealed that E. coli isolates had shown resistance to amoxicillin (85%) and erythromycin (72%). However, they were found to be affected by azithromycin (53%), ciprofloxacin (86%), gentamicin (86%), norfloxacin (80%) and streptomycin (66%). Resistant pattern in relation to broad spectrum antibiotic (i.e., amoxycillin) points to a situation which should be considered carefully and suggests that indiscriminate use of antibiotics for precautionary or therapeutic purposes should be avoided as it could be the cause of increasing antimicrobial resistance.