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Biological Activities of Selected Species of Family Boraginaceae

Thesis Info

Author

Yasir Ihtesham

Department

Deptt. of Plant Sciences, QAU.

Program

Mphil

Institute

Quaid-i-Azam University

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2010

Thesis Completion Status

Completed

Page

77

Subject

Plant Sciences

Language

English

Other

Call No: DISS/M.Phil BIO/2521

Added

2021-02-17 19:49:13

Modified

2023-02-19 12:33:56

ARI ID

1676719305773

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پہلا باب: تنظیم اسلامی

بانی تنظیم ڈاکٹر اسرار احمد کا تعارف

ڈاکٹر اسرار احمد، ۲۶ اپریل ۱۹۳۲ ء کو ضلع حصار، ہریانہ، بھارت میں پیدا ہوئے۔ ۱۹۴۵ء۔ ۱۹۴۶ ء میں حصار ڈسٹرکٹ مسلم سٹوڈنٹس فیڈریشن کے فعال کا رکن اور جنرل سیکرٹری رہے۔

۱۹۴۷ ء میں میٹرک کے امتحان میں ضلع حصار میں اوّل اور پنجاب یونیورسٹی میں مسلم طلباء میں چوتھی پوزیشن حاصل کی۔ ہائی سکول کی تعلیم کے دوران ہی علامہ محمد اقبال (۱۸۷۷ء۔ ۱۹۳۸ء) کی ولولہ انگیز ملی شاعری سے ذہنی و قلبی رشتہ استوار ہوا اور احیائے اسلام کے لیے عملی جدوجہد کی امنگ سینے میں پرورش پانے لگی۔

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( میڈیکل) میں پنجاب یونیورسٹی میں چوتھی پوزیشن حاصل کی۔ ۱۹۵۴ ء میں کنگ ایڈورڈ میڈیکل کالج لاہور سے ایم۔ بی۔ بی۔ ایس کیا۔ ۱۹۶۰ء۔ ۱۹۶۱ء میں منٹگمری (موجودہ ساہیوال) میں حلقہ مطالعہ قرآن و اسلامک ہاسٹل قائم کیا۔ ۱۹۶۲ ء میں والدین کے ہمراہ پہلی بار حج کی سعادت حاصل کی۔

 ۱۹۶۵ ء میں کراچی یونیورسٹی سے ایم اے اسلامیات کا امتحان فرسٹ کلاس فرسٹ پوزیشن میں پاس کیا اور اواخر سال لاہور منتقل ہوکر کرشن نگر ( حال اسلام پورہ) میں ذاتی مطب قائم کرنے کے ساتھ ساتھ مطالعہ قرآنِ حکیم کے متعدد حلقے قائم کیے۔ فروری ۱۹۷۱ ء میں دوبارہ حج بیت اﷲ سے مشرف ہوئے۔ اس موقع پر زندگی کا اہم ترین فیصلہ یعنی آئندہ میڈیکل پریکٹس چھوڑ کر بقیہ زندگی ہمہ وقت دین کی خدمت میں وقف کرنے کا عزم کیا۔

۱۹۷۲ ء میں تعلیمات وافکارِ قرآنی کے فروغ کے لیے مرکزی انجمن خدام القرآن، لاہور کے...

حقِ انتفاع و انتفاعی ملکیت: ایک فقہی تحقیقی جائزہ

There is quite difference between ownership and right of use in other words usufruct, at present this term is widely used in Islamic financial institutions for beneficial ownership. But the use of this term is entirely changed from western law and Islamic law. Particularly in Islamic law, legality of a product or things depends on its objectives. In this article it has been discussed in detail in the light of different school of thoughts of Islamic jurisprudence.  

Utilization of Industrial Wastes for the Production of Amylase from Thermophillic Actinomycetes

Actinomycetes are a facultative thermophilic group of gram-positive bacteria which produce antibiotics and a range of hydrolytic enzymes including amylases, proteases and lipases. Some Actinomycetes are pathogenic but the majority is saprophytic inhabiting soil, water plants and other habitats, where they play an important role in soil structure and composition. In the present study, bacterial strains from three soil samples obtained from a hot water stream were screened for their potential to produce α -amylase. Out of twenty isolated strains, four were selected due to their amylase producing ability as determined through production of a zone of hydrolysis. These strains were identified by morphology, biochemical tests and PCR amplification. Bacterial strain W1 giving a 3.8 mm zone of hydrolysis was identified to be Actinomycete and N3, U1, and U4 giving 3.6, 3.8 and 3.6 mm zones of hydrolysis, respectively were identified as Bacilli. Growth of Actinomycete and amylase production was optimized on four different nutrient media. Highest growth and amylase activity( 3.882 OD and 132.3 AU, respectively after 72 hours at 60oC) were obtained by using medium-4. Conditions for the maximum production of amylase in submerged culture fermentation were optimized using medium-4. It was found that by using 5% inoculum of 30 hours age, amylase activity of 140.8 AU was obtained. Optimization of agitation speed (150 rpm), temperature (60 oC), pH (7.5), starch (2.5 %) and glucose (3%) increased activity from 140.8 AU to 162.4 AU. Growth and amylase production were then optimized by using agro-industrial waste as sole source of carbon and nitrogen in the modified AGS medium. Nine agro-industrial wastes namely soybean meal, wheat bran, peanut meal, mustard meal, sunflower meal, linseed meal, cottonseed meal, corn meal and molasses were used individually and in combinations to optimize amylase production. Soybean meal and wheat bran were found to be more effective nutrient sources with amylolytic activities of 176.2 AU and 171.6 AU respectively. Soybean meal in combination with molasses at 2:1 ratio were found to bethe best carbon and nitrogen sources giving amylolytic activity of 230.6 AU. In solid state fermentation, maximum amylolytic activity( 186.2 AU) was obtained by using wheat bran and molasses in 2:1 ratio at 55oC after 72 hours of incubation. The enzyme was purified 24-fold by acetone precipitation and gel filtration. Single peak during gel filtration indicated presence of only one type of alpha-amylase. Crude and partially purified enzyme find vital application in desizing of cotton cloth. Desizing of cotton pieces 15x15 cm by acid hydrolysis (0.1 M HCl) resulted in 0.7725g removal of starch. This value served as standard for 100% starch removal. Crude enzyme gave 98.39% removal after 90 minutes of soaking at pH 7.0. However, 98.92 % removal was achieved with partially purified enzyme after 60 minutes of soaking. Optimization of temperature and pH revealed that 100 % desizing was obtained at pH 6.0 when incubated at 60oC for 60 minutes. Studies proved to be successful in utilizing agro-industrial waste for producing thermostable alpha-amylase from Thermoactenomyces sacchari and its application as a desizer. The process is recommended for large production of thermostable alpha-amylase in the future.