A Welcome Note from the Editor

We are delighted and proud to welcome you to the second issue of Volume 2. Each article received and accepted is an important contribution to the already existing knowledge in the field of Biomedical Sciences. All the editorial team is excited about the progress of PBMJ as an international journal. As editors, we would like to express our heartiest congratulation to the team and welcome to the authors and readers. We are also grateful to the advisory board and managing editors. We hope that PBMJ can promote the academic and applicable research and improve the research activities and collaborations. We are aware of the bumps along the way, but we are determined to keep pursuing the research goals to meet the high quality standards and move forward with great courage. If you have any suggestions to improve, you may write to us as a reader. In the age of technology, we can actively conversate with the readers and get their feedback to improve the quality with their valuable input. PBMJ will continue to serve the Biomedical Sciences as an outlet for high-quality research. This is an exciting time for the journal and we look forward to working with authors, the Editorial board and the team to make PBMJ as a leading source for work in the space.

Isolation, Identification and Encapsulation of Probiotics from Graps/Spinach and Biological Screening of Their Secondary Metabolites

Probiotics are living microorganisms that confers beneficial effects when taken in adequate amount. Probiotic are the organisms which possess beneficial effect when ingested by the host. Lactobacilli are an extremely important group of probiotic bacteria. The aim of this study is to isolate lactobacillus strains from Vitis vinifera and Spinacia oleracea with de Man, Rogosa and Sharpe (MRS) broth to search for a new effective probiotic strains. Various strains of Lactobacilli identified and analysed for their probiotic properties including acid, salt tolerance and different fermentative test. The test sample showed significant activity against Escherichia coli (88%) Salmonella typhi (85%) and Staphylococcus aureus (84%). The test sample showed good (60%) phytotoxic activity against Lemna minor at 1000 µg/ml. To protect the isolated Lactobacillus strains encapsulation was done in the product’s environment to improve their survival. The results obtained from the different fractions of metabolites were selected for in vivo acute toxicity studies. The toxicological study revealed that the test samples are safe for animal use upto 800 mg/kg. The test sample showed dose dependent anti-nociceptive potentials, the significant effect was observed with 500 and 800 mg/kg b.w dose. The analgesic effect of diclofenac sodium (standard drug) was greater than test sample. The significant (P < 0.01) anti-nociceptive potentials was noticed at 800 mg/kg b.w. of the animal for normal saline (35.50 ± 0.84), chloroform (29.67 ± 1.22), ethyl acetate (30.67 ± 1.22) and (29.00 ± 0.93) chloroform fraction of L. plantarum secondary metabolites. The test sample reduced the motility of GIT in mice. The reduction in GIT motility is dose dependent and maximum effect was produced by 800 mg/kg dose. The test sample caused different degree of movement of charcoal meal in the intestine i.e. 24.17, 20.17 and 17.83cm at the tested doses of 200, 500 and 800 mg/kg, respectively. During isolation and characterization of chemical constituents (2-(3,4-dihydroxyphenyl)-3,5,7trihydroxy-4H-chromen-4-one),(1,2,3,4,5,6-Hexanehexol), (2-(3,4-dimetoxyphenyl)-5,7 dihydroxy-6-metoxy-4H chromen-4-one),(5-hydroxy-7-methoxy-2-(4-methoxyphenyl) 4H-chromen-4-one), (3S,3aS,5aR,9bS)-3,5a,9-trimethyl-3a,4,5,5a,7,8 hexahydronaphtho [1,2-b]furan 2,6(3H,9bH)-dione. Further investigations would help in the suitability of this isolate for human consumption and domestic animals feeds formulations as important probiotic.