سیرت نبوی ﷺ کا اجتہادی پہلو

Establishment of khilafah and tamkeen fil ‘ard means supremacy of the dictates of shari‘ah and socio-political justice on earth. This is one of the basic objectives and prominent messages of the Holy Quran and Seerah of Prophet Muhammad (s.a.w). About khilafah and tamkeen fil ‘ard the Holy Quran expresses as:   -وَعَدَ اللَّهُ الَّذِينَ آمَنُوا مِنكُمْ وَعَمِلُوا الصَّالِحَاتِ لَيَسْتَخْلِفَنَّهُم فِي الأَرْضِ …  -الَّذِينَ إِن مَّكَّنَّاهُمْ فِي الأَرْضِ أَقَامُوا الصَّلاَةَ وَآتَوُا الزَّكَاةَ وَأَمَرُوا بِالمَعْرُوفِ وَنَهَوْا عَنِ المُنكَرِ وَلِلَّهِ عَاقِبَةُ الأُمُورِ.  -هُوَ الَّذِي أَرْسَلَ رَسُولَهُ بِالْهُدَى وَدِينِ الْحَقِّ لِيُظْهِرَهُ عَلَى الدِّينِ كُلِّهِ وَكَفَى بِاللَّهِ شَهِيداً.  Prophet Muhammad (s.a.w) proclaims:   - وَاَللَّهِ لَوْ وَضَعُوا الشَّمْسَ فِي يَمِينِي وَالْقَمَرَ فِي يَسَارِي عَلَى أَنْ أَتْرُكَ هَذَا الْأَمْرَ حَتَّى يُظْهِرَهُ اللَّهُ أَوْ أَهْلِكَ فِيهِ مَا تَرَكْتُهُ. The Holy Quran and the Seerah refer to some underlying milestones on the way of religious nations to status of khalafah and tamkeen fin ‘ard. These milestones may be expressed in an order as: da‘wah [preaching], deen [practices of prophetic teachings], hijrah [migration], ma‘iyyat-ul-Allah [companionship of Allah], qital [wars], nusrat-ul-Allah [divine aid], izhar-ud-deen [domination of deen] and khilafah [inheritance of authority]. This is noteworthy that journey of khalafah and tamkeen fin ‘ard begins with da‘wah [preaching towards deen] and passing through various milestones ends up again at da‘wah, as obvious from ayat-ul-istakhlaf quoted above. Therefore, the seekers of khilafah and tamkeen fil ‘ard should strive hard and keep struggling with the work of da‘wah with dedication in all circumstances and all means as per time and place requirements in lined with the modus operandi of Prophets, particularly Prophet Muhammad (s.a.w), instead of awaiting the status of khilafah and tamkeen fil ‘ard as prerequisite to start with the work of da‘wah and establishment of deen. This paper primarily aims to elaborate the milestones of Muslim Ummah to reach to the status of khilafah and tamkeen fil ‘ard. It also cast light on the objectives of khilafah and tamkeen fil ‘ard. This work provides useful guidance to Muslim Ummah in general and Ahlud da‘wah in particular about milestones and objectives of khilafah and tamkeen fil ‘ard.

Molecular Analysis of Hcv-Rna Genotypes in Beta Thalassemia Patients of Rahim Yar Khan

Hepatitis C is a worldwide problem which lead to the death of millions of people every year. In thalassemia patients, there is more risk of Hepatitis C infection due to multi transfusion in thalassemia patients. There is likelihood that the different Genotypes of HCV may be present in patients of thalassemia due to infection with Hepatitis C during blood transfusion. The main objective of this study was to rule out different types of HCV RNA Genotypes in the patients Beta-thalassemia and to rule out the causative mutation of the patients with Beta Thalassemia. A total of 100 clinically confirmed patients of Beta thalassemia were included in this study those were enrolled at Thalassemia clinic, Sheikh Zayed Hospital/ Medical College, Rahim Yar khan. The screening of blood samples were performed for the detection of anti-HCV antibodies by ICT method. 42 (42 %) samples were found positive and 58 (58 %) were negative for anti-HCV antibodies. The samples with HCV positive results were processed for HCV RNA amplification by Real Time Polymerase Chain Reaction (PCR) method. HCV-RNA was detected in 18 (7.56 %) samples while 24 (10.0 %) samples were HCV-RNA not detected. These Real time PCR assay was run for the characterization of HCV genotype and found that 17 out of 18 samples were of HCV RNA Genotype 3 while single RNA was The HCV RNA Genotype 1. Therefore, HCV RNA Genotype 3 is most predominant type of HCV in Rahim Yar Khan Region of the Punjab province. It can also be depicted that HCV Genotype 3 is the most wide spread strain in Thalassemia patients. There was no strong association of liver function tests was found in HCV positive patients. The DNA from these patients was investigated for screening of genetic variations by Sanger?s DNA sequencing method in selected region of Beta Hemoglobin gene (HBB) i.e. present on chromosome 11. The sequence analysis revealed a single base change (G>C) in intronic region at c.507 in 30% samples while a single nucleotide variation (T>C) at c.58 was found in 70% DNA sequences. The haplotype analysis was carried out and no significant impact of the variation was found on protein structure and function i.e. synonymous change of codon CAT>CAC (His=). So the identified genetic variations in the selected regions of Beta Hemoglobin gene (HBB) have no significant impact on its phenotype in Thalassemia patients from Rahim Yar Khan District of the Punjab, Pakistan.