قرآن مجید کی تلاوت، نقل و حمل اور خط وطباعت میں طہارت کی شرط کا فقہی تجزیہ

The Islamic scholars have identified several rules which deal with the reciting and reading of the Quran and touching/handling the al-Mushaf: the script of the Quran. One of the most important prerequisites, as viewed by the classical Sunni schools of Islamic law, is taharat, which includes both the physical cleanliness—by ablution (wudu) or complete body wash (ghusl) or both—and the purification of thought from allkinds of disbelief (shirk). By extending the rule of taharat, the mainstream Sunni jurists categorically forbid all non-Muslims from touching the Quran; although they are allowed to touch and read its translation as well as to listen to its recitation. This paper analyzes the views of the mainstream jurists and argues for reappraisal of several aspects of the said condition of taharat for both Muslims and non-Muslims, relying upon those jurists whose views are though different from the mainstream but are more practicable and closer to the objectives of the Islamic Sharia today.

Tetra-Primers Based Genotyping of Rs6511720 Polymorphism in Ldlr Gene and Association With Ldlcholesterol Levels

Dyslipidemia is an abnormal amount of lipids (e.g. triglycerides, cholesterol and/or fat phospholipids) in the blood. It is classified as presentation in the body (together with the specific type of lipid that is increased and underlying cause for the ailment (genetic, or secondary to another condition). Low density lipoprotein receptor gene is known as most important in the development of dyslipidemia. In this study, blood from patients (n=25) and normal individuals (n=25) were used to estimate lipid profile and DNA extraction. Lipid profiling was done through spectrophotometer technique. DNA extracted using standard organic method. DNA quantification will be done on agarose gel electrophoresis and nano-drop. Tetra-primers were designed for rs6511720 polymorphism in the LDLR gene to genotype through ARMS PCR technique. Designed ARMS PCR primers will be useful run on agarose gel to identify the genotype. Genotyping data was analyzed using statistical technique. 389bp was achieved in all of the samples which was control band and will be used to produce allele specific bands i.e. of 257bp and 192bp. Though the intensity of control fragments was weak, the samples are correctly genotyped as evident from the sequencing and BLAST results. Statistical analysis has shown the conformance with HW-principle showing that the samples were collected randomly. But association was not found because of low sample size. T-ARMS may be proven as rapid test to diagnose the mutation in LDLR gene causing the early prognosis of the dyslipidemia