مسز سروجنی نیڈو
افسوس ہے کہ ہماری قومی عمارت کا ایک اور ستون گرگیا اور ۲؍ مارچ کو مسز سروجنی نیڈو ہم سے رخصت ہوگئیں وہ عورت تھیں مگر اپنے اوصاف اور خصوصیات میں بہتیرے مردوں سے بڑھ کر تھیں، وہ انگریزی زبان کی نازک خیال شاعرہ، سحرطراز خطیبہ، سیاسیات کی ماہر، جنگ آزادی کی سرفروش سپاہی اور ہندومسلم اتحاد کا عملی نمونہ تھیں، انھوں نے آزادی کی جنگ میں مردوں کے دوش بدوش قیدوبند کی مصیبتیں جھیلیں، وہ فرقہ ورانہ جذبات سے بلند اور صلح کل تھیں، ان کا دل بڑا وسیع تھا، اس میں مسلمانوں کے لئے بھی جگہ تھی، مسلمانوں کے ساتھ ہمیشہ ان کے دوستانہ تعلقات رہے، اور اس فرقہ ورانہ دور میں بھی ان کے خیالات اور طرزعمل میں کوئی تبدیلی نہیں ہوئی تھی، ہندو مسلمانوں کے بعض اختلافی مسائل میں ان کے ذاتی خیالات حکومت کی پالیسی سے مختلف تھے، جن کو وہ صاف طریقہ سے ظاہر نہ کرسکتی تھیں، لیکن ان کی جھلک ان کی تقریروں میں نظر آجاتی تھی، وہ مغربی تعلیم یافتہ تھیں، ان کی ساری زندگی سیاسی میدان میں گزری، اس کے باوجود ان میں نسوانی اور مشرقی خصوصیات موجود تھیں، دل میں نسوانی مہرومحبت، طبیعت میں خلق و مروت، مزاج میں شگفتگی و خوش اخلاقی اور تہذیب و معاشرت میں مغربی آب و رنگ کے ساتھ مشرقی روح جھلکتی تھی، وہ اپنے طرز عمل سے ہندو مسلمان دونوں میں مقبول تھیں اور دونوں کا اعتماد ان کو حاصل تھا، اس لئے ان کی موت دونوں کا قومی حادثہ ہے، ہوا کا رخ دیکھتے ہوئے اب ایسی شخصیتوں کا پیدا ہونا بظاہر مشکل معلوم ہوتا ہے۔ (شاہ معین الدین ندوی، مارچ ۱۹۴۹ء)
The determinants of child marriage are triggered by complex social, economic, cultural, political and legal disparities. This research method used a cross sectional study. The research sample was 192 women who were married in 2018-2019 in the Campalagian District. This study aims to determine the effect of the age of marriage on the health of ibn and infants in the District of Campalagian. Chi-square test was used to analyze data. The results of the bivariate analysis showed that the age of marriage had an effect on the health of the newborn (p value = 0.003). However, the age of marriage on maternal health during pregnancy, maternal health at delivery, use of contraceptive methods, service standards for birth weight, and support from husbands do not have a significant effect. After conducting bivariate analysis using moderator variables, the results showed that. There is an effect of the age of marriage based on the age of the husband (p value = 0.017) and the husband's education (p value = 0.024) on maternal health at delivery. There is an effect of the age at marriage based on the husband's age (p value = 0.023), the wife's education (p = 0.008), and the husband's education (p = 0.009), on the health of the newborn. It can be concluded that the age of marriage has an effect on the health of the mother and baby and/or if it includes the age and education factors of both the respondent and the partner.
Mushrooms have long been used as food and as a cure for many diseases since they form a low-caloric diet with great amount of proteins, vitamins and minerals. In particular, mushrooms are known to contain phytochemicals that could help in mitigating the development of many diseases such as cancer, diabetes and cardiovascular diseases. 3-hydroxy-3-methylglutaryl-CoA reductase is a first rate limiting enzyme in cholesterol biosynthesis. Inhibition of this enzyme is the prime target of drugs used in the treatment of high serum cholesterol levels in humans. Various synthetic drugs being used in controlling hypercholesterolemia are mostly ineffective in vivo and have high cost of production. Further cholesterol-induced oxidative stress has been considered as a major contributor in the development of atherosclerosis, thus the antioxidant activity of the selected mushrooms (Phellinus baumii, Trametes versicolor, Pleurotus osteratus and Agaricus bisporus) was determined by using DPPH (2, 2-diphenyl-1-picrylhydrazy), H2O2 (Hydrogen peroxide) radical scavenging assays and the total phenolic content was determined by using Folin-Ciocalteu method. The results revealed that the P. baumii exhibited significantly higher scavenging activity against free radicals as compared to other mushrooms under study. Moreover, only a few works have investigated the isolation of compounds from mushrooms that are responsible for lowering blood cholesterol levels. In this regard, this work tested the extracts of the selected mushrooms for their ability to inhibit recombinant HMG-CoA reductase isolated from Drosophila melanogaster. To this end, the cDNA-fragment encoding the 54- kDa catalytic domain of Drosophila HMG-CoA reductase was cloned into a modified pET30b (+) vector, named pET30f using the EcoRI and XhoI sites in the 1 2 multiple cloning site. Expression in this modified vector was under the control of a T7 promoter. The expressed recombinant HMG-CoA reductase was purified by using Ni-NTA chromatography and the molecular weight of the purified enzyme was determined to be 115 kDa by gel filtration chromatography. As a result, it was concluded that this catalytically active HMG-CoA reductase enzyme is a dimer. Moreover, kinetic analysis of this recombinant enzyme revealed the KM values of 0.2 ± 0.02 mM for HMG-CoA and 0.14 ± 0.01 mM for the cofactor NADPH. Extracts from the four evaluated mushroom species showed considerable HMGR inhibitory activity, with P. baumii extract being the most potent (Ki = 0.89 ± 0.18 μg/mL), so further investigations were done on this mushroom for the purification of the compounds responsible for inhibiting HMG-CoA reductase by using silica column chromatography and reverse phase semi-preparative-high performance liquid chromatography (HPLC). The masses and molecular formulas of the purified compounds were determined through Liquid chromatography mass spectrometry (LC-MS), Tandem mass spectrometry (MS-MS), Fourier transform ion cyclotron resonance (FT-ICR) and Gas Chromatography mass spectrometry (GC-MS). The isolated compounds in the most active peak from semi-preparative HPLC were Benzamine, 2,3,5,6-tetrachloro-4 methoxy (259 m/z) identified by using GC-MS while the other two compounds with m/z 399.2 and 457.26294 were detected by using LC-MS and FT-ICR respectively. Thus this study successfully demonstrated the use of catalytic domain of the Drosophila HMGR as a potential tool to identify the inhibitors of enzymatic activity. These preliminary studies on P. baumii have indicated the presence of potential compounds in this mushroom that may be helpful against hypercholesterolemia. So, further studies should be done on the structure elucidation of these compounds as well as isolation of other compounds 3 from P. baumii that may contribute to the development of novel therapeutic agents for lowering blood cholesterol levels.