Determinant Factors Affecting Intention of Child Marriage to Adolescents in Banggai Laut

This study aims to look at the determinant factors that affect the intense / intention to do child marriage to adolescents in Banggai Laut. This research method using a cross sectional study. The research sample consisted of 192 teenage students in SMA Negeri 1 Banggai and SMA Negeri 2 Banggai. Chi-square test and multiple logistic regression were used to analyze the data. The results of the bivariate analysis showed that there was an effect of attitude, family drive and behavior control on the intention to do child marriage (p <0.05). The results of the multivariate analysis showed that there was only one variable that had a p value <0.05, namely the family drive variable with p = 0.029. From exp (B) = 4,871> 1 is a risk factor and the Cl value is 95% more than 1 (1,178-20,142) so that OR is significant. This means that respondents who are influenced by family encouragement have a risk of 4,871 times having the intention of engaging in child marriage. It can be concluded that there are many factors that can influence adolescents in engaging in child marriage. All levels of society should protect children together and stop the practice of child marriage in society. The smallest and closest family unit for the child should be able to protect it, not be the main factor that encourages underage marriage.

Sequencing and Analysis of a Cdna Encoding a Putative Coclaurine N-Methyltransferase Cnmt from Aristolochia Fimbriata, a Basal Angiosperm

Alkaloids are produced in plants through various pathways involving several enzymes that lead to diverse alkaloids. One of the most important alkaloid biosynthetic enzymes is coclaurine N-methyltransferase (CNMT) which is an S-adenosyl-L- methionine-dependent methyltransferase (SAM-MTase). SAM-MTases utilize S- adenosyl-L-methionine (SAM) as a cofactor to methylate other molecules. CNMT catalyzes the methylation of coclaurine. Crystal structures of more than hundred SAM-MTases have been investigated. Several O-methyltransferases have been characterized at the molecular as well as structural levels, but there have been very few molecular studies of N-methyltransferases especially about CNMTs. In this study, the amino acids sequence of Aristolochia fimbriata putative CNMT has been determined by isolating and translating the full-length cDNA. In order to investigate the mechanism of methylation by this putative CNMT, three-dimensional homology model has been built and the ligand (SAM) as well as the substrate (S- Coclaurine) has been docked into its active site. Phylogenetic analyses were performed using the MEGA 4.0 software. The phylogenetic relationship of A. fimbriata putative CNMT with their homologs has also been analyzed. In order to identify the putative CNMT gene and determine its function, online similarity searches were performed by BLAST program using the cDNA sequence as well as the putative protein that could be encoded by the gene. All the methods, applied, predicted that the gene identified might be involved in the production of CNMT. The predicted homology model consists of two domains: the N-terminal catalytic core domain and the C-terminal domain. The catalytic core domain has a central sheet of β-strands surrounded by α helices. The catalytic core domain contains binding site for VSAM. The C-terminal domain consists of alpha helices and a few beta sheets creating a pocket for the substrate in between them. The SAM-binding pocket is located next to substrate binding pocket and there is an opening in between these two cavities through which the methyl group of SAM projects towards the substrate. The most important residues involved in the methyl transfer reaction seem to be Tyr-79 and Glu-96.