بحضور استادِ محترم خواجہ غلام قطب الدین فریدی
(سجادہ نشین دربارِ عالیہ گڑھی شریف)
فریدالدینؒ کی حاصل جسے محبت ہے
اُسی فقیر یگانہ سے مجھ کو نسبت ہے
نہیں ہے آپ کو رغبت کوئی بھی دنیا سے
خوشا! کہ آپ کو شعر و سخن سے رغبت ہے
عطا کیا ہے مجھے آپ نے وہ ذوقِ سلیم
مرے خیال میں اب بے پناہ وسعت ہے
ہوئی ہے فکر بہت نعت میں رواں میری
قسم خدا کی یہ اُن کا ہی فیضِ نسبت ہے
رہِ سخن پہ جو میں ہوں چلا تو میرے حضور
قدم قدم پہ مجھے آپ کی ضرورت ہے
مجھے یہ فخر ہے تائبؔ ہوں آپ کا تلمیذ
میں خوش نصیب ہوں میرے لیے سعادت ہے
Just as a man can resort to divorce when he does not love his wife, the Islamic Law (Sharīa„h) gives the woman the right to end her marriage, if she does not love her husband. However, she will have to repay her the dowry paid to her unless there are circumstances in which a judge could force his husband to pronounce Ṭalaq without compensation from his wife. The Family Code puts an end to an abuse of the rule by judges who demanded the consent of the husband. She asserted that the demand for Khula„ was no longer conditional on the husband's acceptance. As a result, the expression "without the consent of the husband" was introduced in 2005, in Article 54 of the Family Code.
In eukaryotes, pre-mRNA has to undergo different processing steps; addition of cap at 5′ end, removal of introns to join functional exons, and addition of poly(A) tail at 3′ end to become fully functional mRNA. These processing events are linked to each other; one process effects efficiency of the other. Addition of polyA tail is not only limited at 3` end but poly A sequences are also found in genes (within exons and introns). The use of one of these intronic PAS results in primary transcript with different 3`UTR. DDX5 (p68) and DDX17 (p72) are RNA helicases performing different cellular functions; mi-RNA processing, transcription, mRNA processing, cell proliferation/transformation, cellular development and cancer. Four human genes MET, BCCIP, TGM2, and SMAD2 were selected to determine the relationship between p68, U1 snRNP, and activation of intronic polyadenylation. Genes were cloned into pGL3-TK vector having Firefly luciferase reporter gene. Mutation was introduced in 5`splice site to block U1. MCF7 cells were transfected with si-RNAp68/p72. After 24 hours cells were co-transfected with WT and Mut plasmids and pRL-SV40 control vector. Expression level of short isoform was determined by Dual Luciferase Reporter assay. The results suggest the role of p68 in IPA activation. Quantitative PCR was performed on uncleaved/total mRNA that confirmed the role of p68 RNA helicase in IPA acting through U1snRNP. To exclude the possibility that IPA is activated by splicing inhibition, si-RNAs against two splicing factors were used. If competitive inhibition of splicing result in IPA activation then should get the same expression level of short isoform with both si-RNAs, but it was not the case. IPA activation was seen only after si-U1 70k treatment while no or little short isoform was observed after si-U2AF65. These results clearly prove that IPA activation is not related to splicing inhibition. Overexpression of p68 and p72 enhanced the IPA event, again confirming the role of p68 in IPA. All experimental results prove that p68 activates intronic polyadenylation by removing U1 from 5`ss. Influence of p68 on IPA is not direct but it is acting through U1.