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Expression Analysis of Fruitful Mads- Box Gene in Brassica Napus

Thesis Info

Author

Sehrish Mustafa

Supervisor

Sumaira Farrakh

Department

Department of Biosciences

Program

RBM

Institute

COMSATS University Islamabad

Institute Type

Public

City

Islamabad

Province

Islamabad

Country

Pakistan

Thesis Completing Year

2015

Thesis Completion Status

Completed

Subject

Biosciences

Language

English

Added

2021-02-17 19:49:13

Modified

2023-01-06 19:20:37

ARI ID

1676719693997

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مولانا عبدالکریم پاریکھ

مولانا عبدالکریم پاریکھ
یہ خبر بڑے رنج و افسوس کے ساتھ سنی جائے گی کہ ممتاز عالم دین اور مشہور ملی رہنما مولانا عبدالکریم پاریکھ ۱۱؍ ستمبر ۲۰۰۷؁ء کو ناگ پور میں وفات پاگئے، جہاں ان کا خاندان گجرات سے آکر آباد ہو گیا تھا، وہ ۱۵؍ اپریل ۱۹۲۸؁ء کو اکولہ (مہاراشٹر) میں پیدا ہوئے تھے، ابتدائی تعلیم حاصل کر کے یہیں کولڈ ڈرینگ ہوٹل میں ملازمت اختیار کرلی، پھر اپنا کاروبار شروع کیا جس میں اﷲ نے بڑی برکت دی اور جلد ہی وہ ناگ پور میں لکڑیوں کے بڑے تاجر شمار کیے جانے لگے۔
کاروباری مشغولیت کے ساتھ علم و مطالعہ اور دین سے بھی ان کو شغف رہا، اسی اثنا میں ان کا تعلق مولانا سید ابوالحسن علی ندویؒ سے ہوا جو روز بہ روز بڑھتا گیا یہاں تک کہ ان کے خلیفہ مجاز ہونے کا فخر حاصل ہوا، مولانا علی میاں ان کی بڑی قدر کرتے اور انہیں اپنے ساتھ جلسوں میں لے جاتے اور ان سے اصلاحی و دعوتی تقریریں کراتے۔
مولانا علی میاں نے پیام انسانیت کی تحریک شروع کی، جس کا مقصد اسلام کے بارے میں غیر مسلموں میں پھیلی ہوئی غلط فہمیوں کا ازالہ اور یہ بتانا تھا کہ اسلام ساری انسانیت کے لیے دین رحمت ہے، اس کی تعلیم امن و آشتی، انسان دوستی، اخوت، بھائی چارگی اور اتفاق و اتحاد کی ہے، فتنہ و فساد اور ظلم و جارحیت سے اس کا کوئی تعلق نہیں، اس تحریک میں مولانا عبدالکریم پاریکھ حضرت مولانا کے دست راست ہوگئے تھے اور ان کی تقریروں سے غیر مسلموں کو بڑا فائدہ پہنچتا تھا۔
مولانا عبدالکریم پاریکھ کی جانب مولانا علی میاں کا اعتنا دیکھ کر ندوے کا ہر شخص ان کا گرویدہ ہوگیا تھا اور وہ ندوہ کے مختلف معاملات میں دخیل اور اس کی کئی کمیٹیوں کے ممبر بھی...

اسلام اور سائنس میں تضاد کا تحقیقی جائزہ

This research is intended to explore the nature of relationship between Islam and Science whether they are compatible with each other or not? By studying and comparing the teachings of Islam and science it has been exposed that both are different in their foundations, nature and scope. Domain of science is limited to the realm of senses whereas Islam provides the knowledge and guidance about materialistic, unseen and metaphysical domain. Foundations of religion are based upon the revelation while science is based upon theories and experiments. Therefore when science reach the reality after passing through trial and error, confirms the teachings of religion, which proves that Islam and science are not only compatible but Islam plays a role of leadership and basic source for the scientific investigations. Moreover after careful review of Islamic and scientific knowledge it is suggested to know the foundations, nature and scope of both to know the relationship between them and to avoid the confusion.

Bacterial Assisted Phytoremediation of Crude Oil-Contaminated Soil

Petroleum hydrocarbons are recalcitrant compounds and their adverse environmental and public health effects demand that efficient and eco-friendly remediation technologies be devised as countermeasures. The synergistic use of plants and bacteria is considered as one of the efficient technologies for the restoration of crude oil-contaminated soil. The studies performed in this thesis were aimed to (ⅰ) isolate and characterize bacteria associated with the plants growing well in crude oil-contaminated soil, (ⅱ) study the effect of augmentation of hydrocarbon-degrading bacteria on plant growth and crude oil degradation in vitro and in vivo. A large number of hydrocarbon degrading bacteria were isolated from the rhizospheric soil, root and shoot interior of grasses (Lolium perenne, Leptochloa fusca, Brachiaria mutica) and trees (Leucaena leucocephala and Acacia ampliceps) vegetated in crude oil-contaminated soil. The rhizospheric soil yielded 22 (59.45%), root interior yielded 9 (24.32%) and shoot interior yielded 6 (16.21%) hydrocarbon-degrading bacteria. These bacteria possessed genes encoding alkane hydroxylase and showed multiple plant growth-promoting activities. Bacillus (48.64%) and Acinetobacter (18.91%) were dominant genera found in this study. Green house studies revealed that augmentation with crude oil-degrading bacteria enhanced plant growth and crude oil degradation. Colonization and metabolic activity of the endophytes were higher in the rhizosphere and endosphere of B. mutica than L. fusca. The plant species affected not only colonization pattern and biofilm formation of the inoculated bacteria in the rhizosphere and endosphere of the host plant, but also affected the expression of alkane hydroxylase gene, alkB. The beneficial plant-bacteria partnership was applied in the vicinity of an oil exploration and production company for the remediation of crude oil-contaminated soil. Bacterial augmentation improved plant growth, enhanced crude oil degradation, and reduced soil toxicity and these were significantly (p < 0.05) higher than those where plants or bacteria were used individually. A positive relationship (r = 0.70) observed xiv between alkB gene expression and crude oil reduction indicates that expression of catabolic gene (alkB) is important for hydrocarbon mineralization. On the basis of in vitro and in vivo studies, it is concluded that for practical application, support of potential bacteria combined with the grasses is more effective approach than the use of plants and bacteria individually. This technology can be applied for effective remediation of crude oil-polluted sites.