Understanding the anti-Mughal Struggle of Khushal Khan Khattak

Khushal Khan Khattak, a seventeenth century Pakhtun writer, poet and swordsman, and his forefathers had served the Mughal for a long time. However, his fortune took a sudden twist when Mughal Emperor Aurangzeb imprisoned him in 1664, and kept him in solitary confinement at Ranthambore fort. After his release from prison, Khushal Khan was a different person. He remained no more a loyal Mughal official afterwards. Although, Aurangzeb Alamgir and a number of Mughal governors of Kabul tempted him several time to accept a position in the frontier areas but he out-rightly declined. This transformation is clearly visible in his poetry. He took up arms against the Mughals in 1673 and declared a war against them despite the fact that some of his family members even his son had sided with the Mughals. He continued his anti-Mughal struggle till his death in 1689. Some of the critics look at the antiMughal role of Khushal Khan with suspicion and have raised a few queries in this connection. This study looks into the circumstances that saw transformation in his outlook towards the Mughals. Then it explores, whether it was a personal vendetta or the start of a collective anti-Mughal Pakhtun struggle. The article looks into various dimensions, nature and direction of his struggle. This research paper is an attempt to evaluate objectively as to why and how Khushal Khan joined the anti-Mughal camp in the borderland area. Some more related questions are also discussed in details in this article.

Molecular Detection and Charaterization of Escherichia Coli Isolated from Raw Cow Milk Samples Collected from District Muzaffargarh

Milk is generally considered as highly nutritious and useful food for all age groups as well as inexpensive and widely available. However, its quality and microbial content are the features which have to be observed. The quality of milk depends on its biochemical composition and hygienic conditions during the collection of milk and its distribution. Milk itself acts as best medium for enhancing the growth and development of different types of microbes because of its composition and presence of water in large quantity. Milk sold in Muzaffargarh district was suspected to be contaminated because of repeated outbreaks of gastroenteritis. In this context, the research was designed to rule out molecular detection and quantitative analysis of Escherichia coli (E. coli) in the milk samples obtained from various tehsils of Muzaffargarh district. E. coli were considered as the most prevalent bacteria of milk and could be the most probable microbe causing this disease. In this research, 100 raw milk samples were gathered in a way that 10 samples each were collected from Jutoi, Alipur, Kotadu, Murad Abad, Choak Qureshi, Ahmadpur, Rohilan Wali, Shahjamal, Khanpur and Muzaffargarh city. Then the samples of milk were cultured on different culture media for bacterial segregation. Identification of bacterial specie was done through gram?s staining and properties of bacterial culture on different selective media. Biochemical tests were also performed which include catalase and coagulase tests. Final identification was performed through PCR and resolution of PCR products by gel electrophoresis. Antibiotic sensitivity test was also performed so as to confirm the susceptibility of E. coli regarding multiple antibiotics. Out of 10 samples collected each from Jutoi, Alipur, Kotadu, Murad Abad, Choak Qureshi, Ahmadpur, Rohilan Wali, Shahjamal, Khanpur and Muzaffargarh city, results had shown the presence of E.coli in 4(40%), 6(60%), 3(30%), 2(20%), 4(40%), 5(50%), 3(30%), 4(40%), 6(60%) and 5(50%) samples respectively. E. coli isolates were amplified by PCR based on 16S rRNA gene. Results of antibiotic sensitivity test revealed that E. coli isolates had shown resistance to amoxicillin (85%) and erythromycin (72%). However, they were found to be affected by azithromycin (53%), ciprofloxacin (86%), gentamicin (86%), norfloxacin (80%) and streptomycin (66%). Resistant pattern in relation to broad spectrum antibiotic (i.e., amoxycillin) points to a situation which should be considered carefully and suggests that indiscriminate use of antibiotics for precautionary or therapeutic purposes should be avoided as it could be the cause of increasing antimicrobial resistance.