سونا دل سنسار سہیلی
بیٹھی ہوں بیزار سہیلی
رستہ تیرا دیکھ رہے ہیں
دل آنکھیں رخسار سہیلی
ساون رت آنکھوں میں ٹھہری
نیر بنے پتوار سہیلی
وقت ہمارا قیدی ہو گا
جیون ہے گھر بار سہیلی
تم سے رنگ فضاؔ کے سارے
تم بن سب بے کار سہیلی
Islam is the only religion which is complete code of life. The needs of human beings have been felt and addressed properly. In spite of created by only one creator humans are different in their potentials, behavior and attitude. Owing to this difference, the incomes of people are drastically different from each other. As a result, a few people are rich and well off whereas some are poor- being up to sustenance level. Therefore, people cooperate with each other to eliminate the class differences of social strata. In this perspective, Islam has given an invulnerable perfect socio-economic system. For mutual cooperation, Islam has also addressed the issue and suggested different steps such as madharabah, partnership and tenancy. Bedsides Islam has also focused on secondary resources of mutual cooperation and support that are typically related to the personal concern. It includes: sacrifice, gift, free lending and Qarz-e- Hasanah etc. It also reflects that the man gives precedence to congregational matters over his individuality which is an ample example of mutual cooperation. In fact, mutual cooperation is one of those key factors on which the edifice of prosperous society is built. All religions and ideologies have focused and emphasized on the mutual cooperation; however, Islam lays extra emphasis to build a strong and thriving human state.
The present work is concerned with the determination of blood parameters, enzyme levels, blood chemistry and pesticide residues in the blood samples from different areas of Karachi in comparison with unexposed persons (i.e., control). Total 45 persons (exposed to pesticides) were taken in this study to assay the pesticides residues in their blood and also observed the morphological and chemical changes in the blood of affected persons. From 45 persons, only 20 persons were found positive and included in the study. Out of ten (10) standard pesticides five differentpesticides were identified in the blood of exposed persons such as cypermethrin, deltamethrin, polytrin-C, malathion and permethrin. The other standard pesticides such as DDE, DDT, match, diazinon and monocrotophos were not detected. In addition blood samples were also collected from ten (10) normal persons (not exposed to pesticides) for the determination of blood parameters, enzymes levels and blood chemistry. Regarding blood parameters approximately all the blood samples had significant variations i.e., (increase or decrease level) possibly due to the cumulative effect of pesticide residues e.g., Majid a young male aged 25 years had been exposed to pesticides for long time and therefore his TLC and platelet counts were found high in addition to low levels of RBC count, Hb, Hct and MCH which probably due to the excessive secretion of enzymes. Similarly, Ghulam Hussain another young male person aged only 08 years had low levels of RBC, Hb, Hct, platelet, MCV and MCH, which may be due to toxic effects of pesticides. In differential leukocyte count (DLC), granulocyte count was found in lower limits in 40 samples and showed normal limits in 05 samples. Lymphocyte count was xi found high in 31 samples. Low limit of lymphocyte was not found in any person. Similarly, monocyte count was found in high limits in 38 samples. Low monocyte count was not noted in any sample and the rest of the samples had normal limits of monocyte. Positive effects of pesticide residues were also observed on the enzyme levels. Regarding enzyme levels GPT, ALP, and ɣ - GT had higher levels in maximum number of samples. Cholinesterase was found high in 6 samples out of 45 samples and the highest was found in case of Farhan and Abdul Rahmanwhere as low limit of cholinesterase was noted in 15 samples out of total samples which may due to prolonged exposure of pesticides. GPT was found in high limits in all the samples. Also ɣ - GT and ALP found high in all the samples. Effect of pesticide residues were also observed on the blood chemistry. Regarding total bilirubin only one sample showed normal limit. The rest of the samples had raised total bilirubin and the highest was found in Javed a young male person aged 12 years. Similarly, in case of bilirubin direct all the blood samples showed increased level. The standard chromatograms of 10 different pesticides such as match, cypermethrin, deltamethrin, polytrin-C, diazinon, DDE, monocrotophos, DDT, malathion and permethrin were prepared on HPLC and their retention time (RT) was noted. Calculation was done for the quantity of pesticides residues from the peaks of chromatograms. Regarding pesticide residues, out of total 45 samples, only 20 persons were found positive for residues. The quantity of pesticide was determined by comparing xii the peak area of the standard pesticide with the sample peaks. After analysis, it was found that out of 20 samples deltamethrin and cypermethrin were found in noticeable amount in 6 samples. Also polytrin-C, malathion and permethrin were found in 2, 1 and 5 samples respectively. The pesticides such as match, diazinon, DDT and monocrotophoswere not detected in any sample.