نواب امیر حسن خان
افسوس ہے کہ گذشتہ مارچ میں نواب صدیق حسن خان کے پوتے اور نواب علی حسن خان کے بڑے صاحبزادے نواب امیر حسن خان نے دفعتہ انتقال کیا، اس خاندان میں کئی پشتوں تک علم و دولت کا اجتماع رہا نواب علی حسن خان خود صاحبِ علم، اہل قلم کے قدردان اور لکھنؤ کی اہم شخصیت تھے، اس لئے علامہ شبلی مرحوم سے اُن کے بڑے گہرے تعلقات تھے، اس تعلق اور علم دوستی کی بنا پر ان کو ندوہ اور دارالمصنفین سے خاص تعلق تھا، ندوہ کے وہ ابتداء سے حامی و مددگار اور ایک زمانہ تک اس کے ناظم رہے، دارالمصنفین کے بھی رکن تھے، نواب صاحب کی زندگی تک ان کا دولت کدہ اصحابِ علم وکمال کا مرجع تھا، مگر ان کی وفات کے بعد اس خاندان کی پرانی شان قائم نہ رہ سکی، تاہم نواب امیر حسن خان نے حتی الامکان قدیم روایات اور پرانے تعلقات کو نباہنے کی کوشش کی، مگر زمینداری کے خاتمہ نے اس خاندان کا بالکل خاتمہ کردیا، ایک زمانہ میں بھوپال ہاؤس کی رونق اور چہل پہل قابلِ دید تھی، اور اب وہ تلک الایام نداولہابین الناس[آل عمران:۱۴۰]کا مرقع بن گیا ہے، نواب امیر حسن خان کی عمر ساٹھ سال کے قریب رہی ہوگی، اﷲ ان کو اپنی رحمت ومغفرت سے سرفراز فرمائے۔
(شاہ معین الدین ندوی، مئی ۱۹۵۶ء)
Islam is the religion of welfare. It emphasizes on accomplishing the rights of human beings concurrently the rights of Allah. Similarly, the government and the member of society jolted regarding this collectively. And everyone is stimulated concerning his responsibilities and adjudges the followers just like one body. Islamic community is just like a cooperative society. It is a duty of everybody to assist the deprived not only financially but also ethically. Until, to meet affably is declared charity. The leader of Islamic state is the responsible for the welfare of everybody. Rather he is bound to feed every being. That is why Haḍrat ʻUmar said that he is liable in the Day of Judgment if a dog is died due to starvation in his regime. In the research paper, the concept of welfare has been manifested in the light of Sīrat-al-Nabī. The method adopted for the research is qualitative as well as descriptive. The Holy prophet urged the believers of Islam on mutual assistance in adverse times. In disaster situations, the responsibilities of every Muslim increase and he should devote his intention tooth, nail and body to rescue.
Characterizing genomic differences at species level is key to understand evolutionary and phylogenetic relationships, morphological and functional diversities and, above all, meaningful and precise taxonomic classifications. The techniques widely used in molecular biology like Amplified fragment length polymorphism-PCR (AFLP-PCR), endonuclease restrictions followed by Pulsed Field Gel Electrophoresis are the techniques mainly used to differentiate species of microbes for many years. These techniques are very useful in determining genomic differences arisen from point mutations, insertion-deletions and inversions. These differences may represent functional dissimilarities in studied organisms. AMPylation enzymes might reveal the changes occurring in proteome of organisms having similar genomes that may further explain the functional diversity of these organisms.
The whole genome sequences of nine Rhizobium species were evaluated with different in-silico molecular techniques such as AFLP-PCR, endonuclease restrictions and AMPylating enzymes diversity for genome comparison. The entire genome sequences of Rhizobium species were retrieved from NCBI and aligned with Progressive Mauve and visualizedas Phylogenetic tree.The AFLP-PCR was carried out in-silico from the insilico.ehu.es, different combinations of restriction enzymes with different nucleotides to generate the AFLP bands to draw phylogeny. Different set of restriction enzymes were used for Restriction Digest and PFGE in silico for scoring of binary scores and analyzed. The post-translational modification (PTM) and ampylating enzymes diversity from the proteome of Rhizobium species were determined from novPTMenzy. The phylogenetic treesbased on AFLP-PCR and PFGE were compared with the tree on whole genome basis and slight variations were observed in the AFLP and PFGE based trees. Results clearly demonstrated the presence of PTM?s i.e. AMPylation, Hydroxylation, Sulfation and Deamidation with their specific domains (ampylating enzymes) GS-ATasE (GlnE), Fic, Doc (Phosphorylation); Aspargine_hydroxylase, Collagen_prolyl_lysyl_hydroxylase; Sulfotransferase and CNF (Cytotoxic Necrotizing Factors), respectively were observed in different Rhizobium species. The results pertaining to post translational modifications are discussed with relation to functional diversities reported in these species.