گو نہ تم سے ملی وفا مجھ کو
پھر بھی تم سے نہیں گلہ مجھ کو
میں بھی تم سے کنارا کر لیتا
کرنی آتی نہیں جفا مجھ کو
زندگانی گزارنے کے لیے
مرنا پڑتا ہے بار ہا مجھ کو
اُس کو مجبوریوں نے گھیرا تھا
کہہ نہ پایا وہ برملا مجھ کو
وہ نہ دھتکارتا مجھے تائبؔ
چاہے کرتا نہ کچھ عطا مجھ کو
The current research was conducted to explore the possible causes of actual employee turnover and turnover intentions. Using Post positivism research philosophy, phenomenological qualitative research method was used to explore the phenomena. Semi-structured interviews of 21 bank employees (selected using purposive sampling) were conducted which were analyzed using NVivo 12. The research findings suggest many uniques themes in order to overcome the problem of employee turnover, especially for banks. The themes which were developed consisted of five significant themes such as the bank appraisals and reward system was identified as biased and based more on favoritism, employee feel that their actual performance is not evaluated properly and sincerely. The other factor concluded by the research findings is that the employees are dissatisfied with the salary and benefits, as they felt that there should a consistent effort to identify employee personal needs which should be customized accordingly in their compensation plans as well. The very essential factor recognized in the research finding was the upward and downward communication gaps with the employees. Such perceptions generated related issues as the employees felt that branches are much deprived to have a direct communication channel with the top team heads. The other very essential factor discovered after the investigation of the phenomena of turnover is lack of career growth. Lastly, another important cause of employee turnover was the transfers, which took place without the consent of the employee. Employees felt demotivated due to such transfers and changes in their work locations. Recommendations and future research directions have been at the end of the research
Insect pests are the major reasons for low yield in agricultural crops. Crop losses due to insect pests can be as high as 25 %, depends upon the climatic conditions and other factors; several strategies have been adopted for the control of insect pests. Recently developed biotechnological applications, several transgenic plants have been developed for insect resistance. Bacillus thuringiensis is one of the most successful examples of insecticidal toxins being used for insect control (especially bollworms). Sucking pests are also major cause of reduced yield in agricultural crops. Sucking insects suck the cell sap from the phloem tissues of plants and also act as a vector for virus transmission. Recent trends in agriculture towards reducing pesticide use and bringing ecological sustainability have led to increased interest in spiders as potential biological control agents. Spider venoms are complex cocktails of toxins that have evolved specifically to kill insects. Spider toxin (Hvt) gene from the Australian funnel web spider which is a calcium channel antagonist has been expressed in tobacco plants to develop resistance against some major insect pests (Bollworms). In the present PhD research work, the main focus was to develop resistance against major insect pests; American bollworm (Heliothis armigera) and mealybug (Phenococcus solenopsis). Different gene constructs were prepared and transformed in tobacco plants (Nicotiana tabacum) through Agrobacterium mediated plant transformation. Spider toxin (Hvt) gene was cloned under phloem specific RSs1 and RolC promoters and lectin gene from onion Allium cepa L agglutinin (ACA) and kallar grass Leptochloa fusca (LfL) was cloned under 2X35S promoter. PCR, Southern hybridization and real time qPCR analysis showed successful transformation and expression of insecticidal toxin genes in N. tabacum. Insect bioassays of transgenic plants were carried out in the laboratory and glasshouse conditions. Results showed 93.75 and 100 % mortality of H. armigera larvae when fed on detached leaves from transgenic tobacco plants expressing spider toxin (Hvt) gene under RolC and RSs1 promoters within 72 hour respectively. H. armigera larvae released on the detached leaves of transgenic plants expressing lectin gene from kallar grass and onion under 2X35S promoter showed, as 75 and 81.25 % mortality within 72 hours respectively. No mortality of H. armigera larvae was observed on the detached leaves of non-transformed tobacco plants up to 72 hours. Transgenic plants expressing spider toxin under RSs1 and RolC promoters gave good resistance against P. solenopsis on detached leaves; 68.75 and 75 % and on live transgenic tobacco plants 70 and 62.5 %, up to 10 days respectively. Transgenic plants expressing lectin gene from onion and kallar grass under 2X35S promoter showed high level resistance against nymphs of P. solenopsis on detached leaves; 87.5 and 81.25 % and on live transgenic plants; 90 and 87.5 % mortality of P. solenopsis within 10 days respectively. No mortality of P. solenopsis was recorded on non-transformed (control) tobacco plants up to 10 days. This study described expression of Hvt gene under RSs1 and RolC promoters and lectin gene under 2X35S promoter. Both genes are helpful for controlling chewing insects like H. armigera larvae and sap-sucking insect like P. solenopsis. This study has a potential for developing insect resistant transgenic crops. These crops can reduce the use of harmful pesticides, fuel use, input cost and yield losses, while increase profitability and enhance biodiversity and save the environment.