طلب معاش کی اہمیت اور اس کی ترقی کے اسباب قرآن اور سنت کی روشنی میں

Trade has been considered as the most important mode of caring livelihood.  Trade is considered as sole biggest medium because all other modes carry secondary position, as they all derive from trade in one way or the other. That’s way crafts and services have gained much success in this era of machinery and technology. In spite of all this trade assumes the same highest positions in this era too. Islam has not left trade on its own but has framed principles. These principles are not only helpful on individual scale but they give benefits to whole community. It benefits in this world and hereafter of its users. Islam is an eternal religion, so it addresses all problems of this era and of upcoming in its teachings. We can gain both worldly and hereafter benefits by acting upon Islamic principles. We have glorious examples of past traders who visited the whole world in order to carry trade. They trade far and wide on the basis of Islamic principles. They not only maintained their businesses, but also preached Islam through their just dealings and humble behavior. Their customers or the people who come into their contact were so impressed that they not only became Muslims but also changed their traditions, Customs, culture and even languages. This article discusses the reasons that explicate the beauty of Trade.

Mutagenesis of Indigenous Streptococcus Equisimilis Isolates for Enhanced Production of Streptokinase

Acute myocardial infarction and ischemic stroke are caused by thrombosis or the obstruction of blood vessels with clots and this is the leading causes of death. The single handling accessible is the use of thrombolytic agent to liquefy the blood lump. Hyper production of streptokinase was carried out in this research work. Beta hemolytic Streptococcus equisimilis was isolated from indigenous sources and then mutagenesis of this isolate was carried out by means of chemicals as well as radiations. To seek the optimum activity, different kinetic and thermodynamic parameters like pH, temperature, Km, Vmax, molecular weight, melting temperature, half-life, enthalpy and entropy etc. were applied on the purified enzyme. UV irradiated strain resulted in 335 U/mL activity with 1116.66 U/mg specific activity, 0.30 mg/mL protein, 41.92 fold purification and 69.79% recovery whereas Sodium azide derived mutant resulted in 400 U/mL activity with 2000 U/mg specific activity, 0.20 mg/mL protein, 71.94 fold purification and 64.51% recovery of the finally purified enzyme. Gamma irradiated strain exhibited 300 U/mL activity with 1428.57 U/mg specific activity, 0.21 mg/mL protein, 59.52 fold purification and 75.94% recovery whereas ethidium bromide derived mutant showed 365 U/mL activity with 1659.09 U/mg specific activity, 0.22 mg/mL protein, 66.52 fold purification and 85.08% recovery. Optimum pH and temperature of the finally purified enzyme was 7 and 45oC. Enthalpy of denaturation (ΔH*) of streptokinase at 450C was 43.67 kJ/mole. The Energy of thermal denaturation ΔG* was 101.14 kJ/mole and entropy of inactivation ΔS* was -197.32 kJ/mole at 45oC. The negative value of ΔS* indicated that streptokinase was thermodynamically stable. Km and Vmax values of streptokinase were 26.31 mM and 50 MS-1. Streptokinase produced from sodium azide derived mutant exhibited activity within the pH range of 6 to 8 while it presented its best performance at pH 7. Thermal stability between 45oC to 80oC was shown by the streptokinase along with half-life of 244 minutes while less stability was shown at 80oC along with 45 minutes of half-life and 40.41 kJ/mole as enthalpy of denaturation (ΔH*).