ڈرتے ہیں بندوقوں والے ایک نہتی لڑکی سے
ڈرتے ہیں بندقوں والے ایک نہتی لڑکی سے
پھیلے ہیں ہمت کے اجالے ایک نہتی لڑکی سے
ملا، تاجر جنرل، جیالے ایک نہتی لڑکی سے
آزادی کی بات نہ کر لوگوں سے نہ مل یہ کہتے ہیں
بے حس، ظالم، دل کے کالے ایک نہتی لڑکی سے
حبیب جالب
Dealings of the Holy Prophet (S.A.W) with non-Muslim show that Islam is the religion of peace, brotherhood, religious tolerance, independence and humanity. Before the spread of Islam the conversations and dealings of the Holy Prophet with non-Muslims forced them to call him Al-Sadiq and Al-Ameen. Sympathy and tolerance in clandestine and public preaching, the style of letters to non-Muslim empires, the dialogues with non-Muslim delegations in Madani era, the orders given for the safety of their wealth, belongings and their life, the tolerance, forgiveness and prayers for the enemies, the good behavior with the non-Muslim prisoners, even when Muslims got dominance over non-Muslims at the time of the conquest of Makkah, the common pardon given by Holy Prophet (S.A.W) are the true example that Islam preaches peace. The attitude of Sahaba-e-Karam (R.A) and the liberty of rights given to Non-Muslims by them- no religion and no community of the World can give this example. The people responsible for maintaining the peace of the world and brotherhood should see how Muslims are treated all over the world? Muslims had given to non-Muslim permission to practice their religious festivities freely and even to wear any type of clothes. The ban of Hijab in France, the punishment of innocent Dr. Afia Siddiqui, the Egyptian pregnant woman (Marwa Sharbeney) had been mercilessly assassinated and her husband injured by knives in the court of Germany because of Hijab, the discussions going on to restrict the domes of Mosques and Adhan (Call to prayer) in Switzerland, the insult of the Holy Quran by Pope Terry John, of Florida, the disrespect of Holy Prophet (S.A.W) etc are the questions awaiting answers from West and United Nation. Islam promotes of peace, equality and religious freedom. It also invites non-Muslims to come and sit on a platform and discuss these problems for the sake of worldly peace to end the religious hatred and to form true peace in the world.
In present study, Thuja orientalis (Thuja) and Cupressus sempervirens (Cypress) were collected from different agro-climatic regions of Punjab, Pakistan. These regions include Rice/ Wheat Punjab zone (Lahore, with semi-arid climate), Mixed-crop Punjab zone (Faisalabad, with desert climate) and Rain fed (Barani) Punjab zone (Islamabad, with warm and temperate climate). The leaves of Thuja and Cypress collected from each region were subjected to hydrodistillation and steam distillation separately, to extract essential oils from them. The yields of essential oils were found to be affected significantly by distillation methods as well as regional variations. The initial TLC analysis of these essential oils showed that these samples were consisted of different polarity groups, ranging from highly polar oxygenated compounds to semi-polar or non-polar terpene hydrocarbons. The chemical composition of all of these essential oils was studied by GC/MS which showed the presence of different major components in different essential oils, depending on regional variations and distillation methods. These essential oils were fractionated into different fractions by vacuum distillation fractionation and high speed counter current chromatography (HPCCC). The results of different biological activities showed that all of these essential oils and fraction were having significant antioxidant, antibacterial and antibiofilm potential. Also, the fractions were mostly found to be more active than pure essential oils. The results of GC/MS analysis of most active fractions showed that vacuum fractions were mainly consisted of groups of compounds with two or more compounds in higher percentage while HPCCC fractions were consisted of relatively pure compounds with higher concentration. The bioactive compounds which were isolated from different fractions of Thuja and Cypress essential oils were α- Pinene, α- Cedrol, Δ-3-Carene, b- Pinene, α-Terpinyl acetate, α- Humulene, cis-Limonene oxide, and Isoaromadendrene epoxide.