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Home > Polymorphic Variation and Phylogeny of the Myostatin Gene Exon 1 in Wild Punjab Urial Ovis Vignei Punjabiensis

Polymorphic Variation and Phylogeny of the Myostatin Gene Exon 1 in Wild Punjab Urial Ovis Vignei Punjabiensis

Thesis Info

Access Option

External Link

Author

Asad Ullah Ghalib

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=119

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720971129

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Myostatin encoded by MSTN gene is very important growth factor related to the ? super family. Its expression causes the fat production and muscle deregulation. The variation in MSTN gene has been associated with distinction in muscularity in animals. Double muscling can be observed in several farm animals including goat, sheep, pig, cattle, horse, and also in fish. The knowledge of variation in MSTN gene is necessary to be explored in wild animals. In the current study we hypothesized that the variation within the MSTN gene may be important in identifying the genomic region that would be responsible for significant phenotypes. Therefore the MSTN gene previously referred as GDF8 gene was studied in the Punjab Urial, considered it as one of the wild ancestors of domestic sheep found in the North of the Punjab province of Pakistan. The samples from wild and captivity were subjected to DNA extraction, PCR and sequencing. The sequenced product (373bp) includes 5? untranslated region (UTR), 67 base pair upstream from the start codon (ATG), complete exon 1 of the MSTN gene and partial intron 1 region. The coding region contained 372 bp. In untranslated region one substitution and one indel (deletion) was observed. One substitution (A>C) was reported on position 3564 of the MSTN gene and the indel (deletion) was identified at 3574 bp position. All the sequences were compared with the reported sequences on GenBank. The analysis shows the presence of thiamine in the wild sequence .This is the preliminary study of MSTN gene in the wild Punjab Urial. Further studies might give us a deep insight of important MSTN gene in wild as well as domestic animals.
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نولکھی کوٹھی

فقیر بستی میں تھا

                ناطق نے ایم فل اردو منہاج یونیورسٹی لاہور سے کیا اور ان کا مقالہ محمد حسین آزاد پر تھا۔ اسی مقالہ کو انہوں نے دوبارہ تھوڑا تبدیل کرکے ایک نئے نام سے شائع کروایا یعنی فقیر بستی میں تھا یہ ایک سوانحی ناول ہے جسے انہوں نے اپنی تخلیقی مہارت کے ذریعے ایک نیا روپ دیا۔ ’’فقیر بستی میں تھا‘‘عکس پبلی کیشنز لاہور نے 2019ء میں چھاپا۔ناطق نے نہایت خوبصورتی سے اس کتاب کو 45 حصوں میں تقسیم کیا ہے اور اپنے ہر ایک عنوان میں آزادزندگی کا پہلو بیان کیا گیا ہے۔کتاب کا انتساب آغا سلمان باقر کے نام لکھاہے۔کتاب نہایت مہارت سے لکھی گئی ہے پڑھتے ہوئے محسوس ہوتا ہے کہ لکھنے کیلئے کہاں کہاں کی خاک چھانی گئی ہوگی۔انہوں نے  آزاد کی شخصیت کے تمام پہلوؤں پر روشنی ڈالی ہے ۔ایسا محسوس ہوتا ہے کہ ناطق کی طرح قاری بھی کتاب پڑھ کر آزاد کا عاشق بن جائے گا۔اس ضمن میں شہناز نقوی لکھتی ہیں :

’’مجھے یوں محسوس ہوا جیسے میں کوئی seasonsدیکھ رہی ہوں ، عمدہ طرز بیان کو پڑھنے والا بھی مولانا آزاد کے ساتھ ساتھ خود کو محسوس کرنے لگتا ہے۔کتاب کا اسلوب اتنا پرکشش ہے کہ قاری کو کہیں بھی بوجھل پن محسوس نہیں ہوتا ورنہ تحقیقی کتب پڑھناذرا مشکل مرحلہ ہوتا ہے۔‘‘(15)

                انہوں نے آزادکی  زندگی کو اس طرح لکھا کہ قاری بھی آزاد کا دیوانہ ہوجائے ناطق نے خود بھی آزاد کی کتاب جب آب حیات پڑھی تھی تو بار بار اس کتاب کو تقریباً چالیس مرتبہ پڑھا،اسی لیے ایم فل اردو میں اس عنوان پہ مقالہ تحریر کرنے کا فیصلہ کیا۔

Origin of Earth: A Quranic Perspective

The modern scientific concepts were clearly envisioned in the Qur'an date back to 7th century, when observational facilities were not available, mathematical principles were not discovered, primary principles (quarks) and fundamental forces  i.e. Gravitational and electromagnetic were not identified. The word earth (أرْضَARD) appears 409 times in Quran. The number of verses in which the Earth is mentioned first is quite small, e.g. Sura 2, verse 29 and sura 20, verse 4, where a reference is made to "Him Who created the earth and the high heavens". On the other hand the numbers of verses where the Heavens are mentioned before the Earth are much larger e.g. Sura-l-araf (7), Verse 54; SuraYunus (10), verse 3; SuraQaf (50), verse 38; Sura l-Hadid (79), verses 27 to 33 etc. The Quran describes itself as a book of guidance. Sura l-Baqarah, verse (67)[i] states:  قَالَ أعُوْذُ بِاللهِ اأنْ أَکُوْنَ مِنَ الْجَاهِلِيْن He said, "I seek refuge in Allah from being among the ignorant."The Qur'an does not render a coordinated description of the Earth Creation. In lieu of a continuous story of creation, there are verses dispersed all over the Quran which deal with certain aspects of the Earth Creation. These scattered verses provide information on the sequential events marking its development with varying degrees of detail. The Quran referred toward at least five points which shed light on the creation of heaven and earth.  And these points are entirely confirmed by scientific facts.    

Geographic Variation of Helicoverpa Armigera Hubner Lepidoptera: Noctuidae Susceptibility to Cry Toxins in Bt Cotton, in Punjab Pakistan

Bt transgenic cotton, being a vital part of pest management effectively controls the target pest, Helicoverpa armigera. However, the success of Bt technology depends on the persistent vulnerability of target pests to Bt insecticidal protein The results of field studies exhibited a parallel trend of H. armigera egg laying in all six cultivars whereas larval incidence found minimum in dual toxin genotype followed by single Bt genotypes (FH-118 followed by FH-Lalazar, MNH-886 and FH-142, respectively) and maximum on non-Bt (FH-942) at Faisalabad, Multan and Bahawalpur during both study years. The baseline susceptibility of H. armigera larvae conducted in 2013, showed LC50 ranging from 0.123 to 1.026 µg ml-1, 0.148 to 1.675 µg ml-1 and 0.210 to 2.761 µg ml-1, against 1st, 2nd and 3rd instar larvae respectively to susceptible and field collected populations (Faisalabad, Multan and Bahawalpur). These results gave an indication that Bahawalpur population was found 8.34, 11.32 and 14.71-fold resistant, followed by Multan (5.54, 7.44 and 8.99 fold) and Faisalabad population (4.08, 4.88 and 5.23 fold), for 1st, 2nd and 3rd instar larvae respectively in comparison with susceptible population. The MIC50 was 0.003 to 0.006 µg ml-1, 0.009 to 0.088 µg ml-1 and 0.014 to 0.206 µg ml-1 for 1st, 2nd and 3rd instar larvae respectively. whereas highest number of BWP population survived and showed resistance (23.33, 9.77 and 14.71-fold), MLT (10.67, 5.88 and 6.21) and FSD (6.67, 2.44 and 2.71 fold). This lethal concentration increased gradually and similar trend were found during 2014. In case of detached plant bioassay, 1st and 2nd instar larvae were found to be more susceptible and highest mortality was recorded on different plants parts of CEMB33 (leaves > square > flower > bolls), compared with single Bt cotton genotypes (FH-118 > FH-Lalazar > MNH-886 > FH-142, respectively). All 1st and 2nd instar larvae were unable to pupate. The order of mortality was: susceptible > Faisalabad > Multan > Bahawalpur. In case of bioassay on 3rd, 4th and 5th instar, the percent mortality and female longevity was consistently decreased whereas the pupation, adult emergence, fecundity and male longevity was consistently increased. The data also indicated that 1st instar neonate died (i.e. 100% cumulative mortality) after one day, three days and six days when assayed by 4, 1 and 0.25 µg ml-1 toxin respectively. However, maximum survival was recorded when neonate assayed at 25ᴼC followed by 45, 30, 35 and 40ᴼC, in case of all preceding concentrations (0.063, 0.016 and 0.004). The pupation (%), adult emergence (%), pupal weight (mg), larval and pupal duration (days) was recorded maximum at 25ᴼC followed by 45, 30, 35 and 40ᴼC. The Relative Enhanced Toxicity Factor was increased gradually and consistently up to 40ᴼC but decreased suddenly when assayed at 45ᴼC, in case of LC50 and LC90 estimation.The results of asymmetric fluctuation indicated that right side of tibia, fore wing and hind wing length was larger than the left side. However, the susceptible population possess minimum length of tibia, fore wing and hind wing than population of Faisalabad, Multan and Bahawalpur. The data showed that all the morphological traits including FMR ratio was found maximum from Bahawalpur population followed by Multan, Faisalabad and susceptible population.