Search or add a thesis

Advanced Search (Beta)
Home > Effects of Doxycycline Stabilized Platinum Nanoparticles on Staphylococcus Aureus & Staphylococcus Pyogenes

Effects of Doxycycline Stabilized Platinum Nanoparticles on Staphylococcus Aureus & Staphylococcus Pyogenes

Thesis Info

Access Option

External Link

Author

Sehrish Latif

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=135

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720975222

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


The facile method was used to synthesize platinum nanoparticles (Dox-Pt (0) NPs) which are stabilized by doxycycline. Platinum nitrate (PtNO3) with the addition of doxycycline was used to synthesize platinum (Pt) nanoparticles. The synthesized platinum nanoparticles were selected for antimicrobial activity using the qualitative well diffusion method by measuring the inhibition zone in mm against Gram positive bacteria such as S. aureus and S. pyogenes in Tryptic soy agar medium. The sterilized agar media was poured into Petri plates and allowed to solidify. On the surface of the media fresh microorganism cultures (108CFU ml-1, CFU, colony-forming units) were spread with the help of sterilized L-shaped glass loop. A cylinder glass pipette of 5 mm diameter (pre-sterilized) was used to bore cavities. The UV-vis-absorption spectroscopy was used to confirm the accumulation of (Dox-Pt (0) NPs) by exciting the distinctive Plasmon absorption maxima at 267nm. X-ray powder diffraction pattern confirmed the crystalline nature of prepared Pt (0) NPs. Scanning Electron Microscopy (SEM) was used to study the synthesized nanoparticles of platinum. These Doxycycline stabilized platinum nanoparticles were used to check the antibacterial activity of Gram positive bacteria Staphylococcus aureus and Staphylococcus pyogenes by comparing it with Doxycycline. The results showed the great tremendous effect against Gram positive bacteria Staphylococcus aureus and Staphylococcus pyogenes. These Doxycycline stabilized platinum nanoparticles (Dox-Pt (0) NPs) also showed its tremendous effects against anti-cancer therapies.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

۱۷۔ واپسی

واپسی

وہ شخص اک مدت بعد لوٹا

جب مہجور رات کی خاموش پہنائیوں میں

پلکوں کی چلمنیں تھک کر گر چکی تھیں

آنکھوں کے منتظر دَر بند ہو چکے تھے

دل کے طاقچے میں انتظار کا چراغ بجھ چکا تھا

دھویں کی پرچھائیاں ڈرا رہی تھیں

امید کا چاند ڈھل چکا تھا

تنہائی بین کر رہی تھی

خواب ٹھوکریں کھا رہے تھے

ہر چیز علم بغاوت لہرا چکی تھی

...

The Preservation and Authenticity of Ḥadīth As a Source of Sīrah: A Critique of Robert Spencer’S Views on Historicity of Muḥammad

The account of the deeds and sayings of the prophet (ﷺ) has been carefully preserved since the dawn of Islam. This is what actually led Ernest Renan to believe that “[Islam] was born in the full light of history”, and that the life of Muḥammad (ﷺ) can be known as explicitly as that of any sixteenth century reformer. Notwithstanding, some contemporary writers doubt the historicity of Muḥammad (ﷺ) ; Robert Spencer is one of them. He is an American author. He is quite famous for his criticism of Islam and the prophet of Islam. He has published twelve books so far. He is a member of Melkite Greek Catholic Church. He contends that Muḥammad’s (ﷺ) historical value is in no way greater and more reliable than mythological figures or characters such as Robin Hood and Macbeth. He maintains that the very idea of subjecting the traditionally accepted account of the life of Muḥammad (ﷺ) and the origins of Islam will be regarded by many Muslims as an affront. To substantiate his argument, he rigorously criticizes the historical value of ḥadīth. He argues that aḥādīth were fabricated and compiled in the third century. He maintains that aḥādīth were concocted much later to be used as weapons in political strife and religious controversies. This paper is an academic attempt to prove that aḥādīth were painstakingly preserved and for that purpose various techniques were employed. Besides, it also sheds light on the reasons for the fabrication of aḥādīth and the ways and means utilized for sifting the genuine aḥādīth from forgeries. Findings of this article suggest that the minute details of the life of Muḥammad (ﷺ) have been scrupulously preserved; hence aḥādīth can be used as a reliable source of Sīrah.

Identification, Population and Food Habits of Marmots in District Neelum, Azad Jammu and Kashmir

Marmots belong to Order Rodentia and Family Scuridae. Fourteen species of marmots are reported from northern hemisphere of the world, living in alpine meadows which have long, harsh winter season. Two species of marmots have been reported from Pakistan, Marmota caudata and Marmota himalayana. Marmots are prey species for carnivores of alpine region, a good nutrient recycler and primary consumer converting biomass to energy. Marmots are least studied animals in Pakistan and knowledge gap about its ecology exists all over its range in the country including Azad Jammu & Kashmir (AJ&K). Present study was conducted from June 2011 to November 2013 in district Neelum of AJ&K, located at 34°. 28 to 34°.48 N and 73°. 44 to 74°. 58 E, covering an area of 3621 km2. Objectives of the study were to identify the species of Genus Marmota from district Neelum; to determine its distribution range in the study area, to collect demographic data of Marmota spp. along with its population density and to analyze diet composition and food availability of Marmota spp. in the study area. For data collection on distribution and population of marmots, 10 study Sites (Baboon, Bloar Kassi, Commu, Mianwich, Janwai, Lawat Patlian, Noori Nar, Seen Gull, Shakar Garh, Shounter) were selected in the study area, based on the information collected from Department of Wildlife and Fisheries AJ&K, local residents and nomads in the area. Animals were live captured using body snares to collect blood samples for DNA analysis, to gather morphometric data and to determine their over winter survival rate. Data about its distribution was collected through line transect surveys. Demographic data was collected from the animals 1 2 captured during two consecutive years i.e. 2011 and 2012. Data on animals captured during two consecutive years (2011 and 2012) were used to gain basic insights about overwinter survival and reproduction. To estimate density of marmots, we relied on distance sampling. Data were analyzed using program SPSS 16.0. ANOVA was used to compare the means of morphometric measurements. Student’s t test was used to analyze data and Duncan’s multiple range test (MRT) was used to analyze food preference of marmots. Dissimilarity coefficient was calculated to assess food preference of marmots. BatchPrimer3 V 1.0 was used to design the primers while Chromas lite, MEGA 5 and UGENE software were used to align and analyze the Sequences. Distance 6.0 software was used to analyze the data of distance sampling. Morphometric data and DNA sequencing results suggested that marmot species existing in district Neelum was a variant of golden marmot (Marmota caudata aurea). The patchy distribution of the species was recorded form alpine zone of district Neelum within the altitudinal range of 3120 m to 3884 m asl. A total of 551 animals were recorded from the study sites having population density of 0.102 ha-1 from Baboon, 0.472 ha-1 from Bloar Kassi, 0.319 ha-1 from Janwai, 0.328 ha-1from Lawat Patlian, 0.473 ha-1from Noori Nar, 0.152 ha-1 from Shakar Garh and 0.224 ha-1 from Shounter. Morphometric measurements of 50 adult golden marmots (male, n= 33 and female, n= 17) revealed total length of adult males ( =74.87; P=0.559) and females from 73.0–77.83 ( = 76.47 cm), showing non-significant difference (P = 0.536 = 74.87). Body mass ranged from 4.3 to 6.6 kg for both sexes and females ( = 5.67 kg) have no significant difference from males ( = 5.57 kg; P = 0.559). Distance between anus and genitalia for males 3 were significantly additional ( = 2.35 ± 0.62 more cm) than females ( = 1.15 ± 0.40 cm; P< 0.001) while the distance of anus and genitalia was non-significant between sub-adult males and females. Average body temperature of captured animals were significantly higher for males ( = 100.78°F) than females (99.47°F; P< 0.018). Over winter survival rate for marmot population before hibernation from Shounter (n = 26.66) and Noori Nar (n = 22.5) has non-significant change than post hibernation from Shounter (n = 24) and Noori Nar (n = 25). Average social group size of golden marmots was recorded as 2.8 animals. Immergence of marmots at Noori Nar was on 15th, 8th and 13th October during the years 2011, 2012 and 2013, respectively. At Shounter during years 2011-2013 emergence was 7th, 9th and 13th May while immergence for hibernation was 24th, 20th and 18th October, respectively. Hibernation period for the marmots was recorded as seven months on average. Diet composition analysis suggested that Golden marmot prefers some plants over the others for food. From Noori Nar, Ligularia sibirica (RD = 3.82), Sibbaldia cuneata (RD = 44.47) and Dactylis glomerata (RD = 2.89) were highly preferred plants based on Relative Density, while Ligularia sibirica (RF = 3.69), Sibbaldia cuneata (RF = 3.97), and Dactylis glomerata (RF = 3.15) were preferred plants on the base of Relative Frequency. Highest consumption of Primula calderiana (RF = 2.72) was recorded on the basis of RD, whereas Doronicum roylei (RF = 2.99) was the most preferred species on the basis of RF. Lowest preference was recorded for Dactylis glomerata (RF = 2.12) and Sisymbrium mollissimum (RF = 3.97) from Shounter. Findings of this study would help ecologists, field biologists and wildlife managers for the conservation of Marmot species in the region, supporting its role as food source for threatened Snow leopard and other carnivore species found there. A long term study to understand population trend, threats and impact of global warming on this species is suggested.