The facile method was used to synthesize platinum nanoparticles (Dox-Pt (0) NPs) which are stabilized by doxycycline. Platinum nitrate (PtNO3) with the addition of doxycycline was used to synthesize platinum (Pt) nanoparticles. The synthesized platinum nanoparticles were selected for antimicrobial activity using the qualitative well diffusion method by measuring the inhibition zone in mm against Gram positive bacteria such as S. aureus and S. pyogenes in Tryptic soy agar medium. The sterilized agar media was poured into Petri plates and allowed to solidify. On the surface of the media fresh microorganism cultures (108CFU ml-1, CFU, colony-forming units) were spread with the help of sterilized L-shaped glass loop. A cylinder glass pipette of 5 mm diameter (pre-sterilized) was used to bore cavities. The UV-vis-absorption spectroscopy was used to confirm the accumulation of (Dox-Pt (0) NPs) by exciting the distinctive Plasmon absorption maxima at 267nm. X-ray powder diffraction pattern confirmed the crystalline nature of prepared Pt (0) NPs. Scanning Electron Microscopy (SEM) was used to study the synthesized nanoparticles of platinum. These Doxycycline stabilized platinum nanoparticles were used to check the antibacterial activity of Gram positive bacteria Staphylococcus aureus and Staphylococcus pyogenes by comparing it with Doxycycline. The results showed the great tremendous effect against Gram positive bacteria Staphylococcus aureus and Staphylococcus pyogenes. These Doxycycline stabilized platinum nanoparticles (Dox-Pt (0) NPs) also showed its tremendous effects against anti-cancer therapies.