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Seroprevalence of Mycoplasma Gallisepticum Among Commercial Poultry Flocks in Chakwal District, Pakistan

Thesis Info

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Author

Shujjah Haider

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=138

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720976010

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Pakistani poultry industry represents one of the largest agro based segment of economy which is growing continuously, providing numerous opportunities for the spread of multiple diseases in the absence of control measurements. Respiratory diseases are continuing to cause heavy economic losses in the poultry industry and increase the overall cost of production in terms of the provision of services of qualified veterinary personnel and the cost of medication for possible treatment. It is therefore important to reduce if not eliminate, respiratory infections among poultry flocks to the barest minimum to have good production and maximize profit of the producer. Various pathogens may initiate respiratory disease in poultry, including mycoplasmosis caused by Mycoplasma gallisepticum (MG).The present study was conducted in 25 commercial layer flocks of hyline and leghorn breed in Tehsil Chakwal, Tehsil Talagang and Tehsil Kallar Kahar, Chakwal District, Punjab Pakistan. Totals 358 blood samples were collected and subjected to serological test. The overall seroprevalence of MG was found through iELISA and SPA as 29.88 % and 20.67% respectively. Further, high prevalence of MG was recorded in 24-31 weeks old layers (44.17%) as compared to 55-63 weeks old layers (14.49%). Lack of vaccination, MG positive breeder flocks or improper biosecurity measures may be the possible reasons behind this high prevalence. The study of seasonal effect on MG infection in birds suggested that high MG prevalence in winter than summer. Moreover, our findings revealed highest MG prevalence in December (44%) while lowest in October (20%). It can be assumed that low temperature increase susceptibility of layers for MG infection. Furthermore, higher prevalence was recorded in layer flocks (33.33%) with large number of birds (4000-5000) than flocks (24.28%) with small number of birds (1000-2000). In addition, it was also found that iELISA test is more sensitive and specific for detection of MG antibodies in serum samples as compare to SPA test although mostly SPA test is being used for serological screening of flocks in field. This evidence emphasize the need of further detailed and more systemic approaches for MG distribution and prevalence investigation to design effective control strategies. A comprehensive epidemilogical surveys for the detection of MG and evolution using molecular tools will improve the understanding of the global epidemiology of the infection.
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غزل

جو دیپ پلکوں پہ دھر رہا تھا

وہ شب کا کشکول بھر رہا تھا

 

چہار سو  نور  تھا  زمیں  پر

کوئی فلک  سے اتر  رہا   تھا

 

اُدھر بھی تو ایک زندگی ہے

وہ جی رہا ہے جو مر رہا تھا

 

ہوائے شب اشتعال میں تھی

چراغ  جلنے  سے ڈر  رہا  تھا

 

ہماری آنکھوں میں تشنگی تھی

کہ  دل  کا  دریا  اتر  رہا   تھا

 

سمیٹتا کیا وہ  مجھ  کو  آ  کر

جو خود ہی ہر  پل بکھر رہا تھا

 

جو  آ  رہے  ہیں  شفیق  آصف

میں  یاد  ان  کو  ہی  کر   تھا

دلالة النص على مبادئ الشريعة الإسلامية في الدساتير العربية وأثره في تنظيم مبدأ المساواة بين المواطنين

This research is about the effect of text meaning on the constitutional rule that related to the Islamic Sharia principles. The Islamic Sharia principles are the source of legislation in the Arab constitutions. It will also look in the impact of this constitutional rule on organizing the principle of equality among citizens in various fields; equality in front of the law and judicature, and equality in rights and freedoms. In particular, this research highlights how the impact of text on the constitutional rule affects on organizing the equality between Muslim and non-Muslim citizens, also between men and women of Muslim citizens. Thus, this research has been split into four topics that addressed respectively as follows: First, what is Islamic Sharia and Legislation in Islamic Fiqh (doctrine). Second, the semantics of multiple formulations of the Islamic Sharia principle as a source of legislation in the constitution. Third, the principle of equality in both, the Islamic Sharia and the positive law. Finally, the impact of the text on the Islamic Sharia principle of Arab constitutions in organizing the equality principle among citizens. The research concluded with set of results that can be summarized as follows: Every formula that has been used in the constitutional rule of the Islamic Sharia principle in the Arabic countries has a different semantics and results that lead to different obligations in the way of organizing the equality principle in the legislative bodies either in constitution's document or in ordinary laws. Also, the research concluded that the Islamic Sharia as a public principle doesn't conflict with the positive law in recognizing the equality principle among citizens in different fields, but the conflict is in the understanding and application of some fields of equality among citizens in different religions and genders. The researcher came with set of recommendations including clarifying wording of the text that related to the Islamic Sharia principle in the Arab constitution rules. These clarifications should prevent any other interpretations. In addition, the researcher suggested to adopt this formula: the original principles of Islamic Sharia is the main source of legislation. Also, formulations of equality principle among citizens in all its manifestations in the constitutions must be consistent with the original Islamic Sharia principle. Also, all the texts that related to the equality among citizens, and between men and women should included the sentence "with no conflict with the original Islamic Sharia principles".

Fruit Quality and Storability of Kinnow Mandarin Citrus Reticulata Blanco in Relation to Tree Age

Rind quality is indispensable for the external appearance and marketability of citrus fruit especially for fresh consumption. Among many factors affecting citrus rind quality, tree age is the most important one, but remains unexplored so far. This study was carried out during 2007-11 and comprised of two parts. The first part of study includes the experiments exploring fruit quality in relation to different tree age, canopy position and fruit size. This study also revealed the cell number, cell size, endogenous nutrients and fruit pectin concentrations (rind and rag) in relation to tree age during fruit growth and development. A comparison of fruit quality of different age groups (3 year, 6 year, 18 year, 35 year) showed that fruit obtained from young trees (3-year-old) were poor in fruit quality such as having more rough rind, rind thickness, rind mass (%) and less juice mass (%), TSS and acidity. Moreover, fruit from young trees had lower rind macro nutrient concentrations (P, Ca) and higher rind micro-nutrient concentrations (Mn and Fe). Fruit in internal canopy position had significantly better fruit quality (smooth rind, less rind thickness, more juice contents) whereas, those in external canopy position were better in biochemical fruit quality such as (TSS), titratable acidity (TA), sugars and ascorbic acid (AA) concentrations. Large sized fruit had more rind mass (%), rind thickness, and lower juice mass (%), TSS, TA (%) and TSS:TA ratio during ambient (20±2°C and 60-65% RH) and cold storage (4±1°C and 75-80% RH) conditions. From nutritional aspects, during fruit growth and development, fruit from six- year-old trees were nutrient deficient in rind (N, P and K), rag and leaf (N) concentrations, while 18-year-old trees were deficient in rag and leaf nutrient (P, K) concentrations. Pectin analysis showed that fruit from 6-year-old trees were deficient in rind total pectin, protopectin and rag protopectin concentrations, whereas fruit from 35-year-old trees were higher in rind water soluble pectin (WSP), total pectin and fruit from 18-year-old trees were higher in rind total pectin and rind and rag WSP. Anatomical studies of tissues from different age groups demonstrated increased cell number with lower cell size in rind tissue of 6-year- old trees in comparison with 18 and 35-year-old trees. Correlation analysis revealed that leaf N contents correlated positively with cell size in fruit from 18 and 35-year-old trees. Cell size was negatively correlated with rind P concentrations and positively correlated with leaf P 1concentrations in fruit from all tree age groups. In second part of the study, the potential of exogenous application of PGRs and nutrients were explored in improving fruit quality of young ''Kinnow'' orchards. The PGRs like gibberellic acid (GA 3 ), 2, 4 dichlorophenoxy acetic acid (2, 4-D), putrescine (Put) and spermine (Spm) were applied before and after colour break to young (3-4 years old) ''Kinnow'' mandarin trees and their influence on fruit quality under ambient (20±2°C and 60-65% RH) and cold storage (4±1°C and 75-80% RH) conditions was determined. Only 2, 4-D significantly improved TA (%) and TSS:TA ratio during shelflife studies. GA 3 and Put treated fruit exhibited maximum mass loss (%) during shelf studies and cold storage respectively. GA 3 10 mg L -1 and cytokinins (kinetin and benzyladenine) 30 mg L -1 applications at fruit setting stage significantly improved juice (%) and reduced rag (%). In nutritional experiments, sulphate of potash (SOP), single super phosphate (SSP), urea, calcium ammonium nitrate (CAN), Wokozim and Isabion were applied to improve fruit quality. Wokozim application reduced rind thickness and improved reducing sugars of fruit, SSP improved juice contents, ascorbic acid (AA) and reduced rind mass (%) although not significant than control; SOP improved AA concentrations in ''Kinnow'' mandarin juice and CAN improved TSS and AA concentrations. In conclusion, tree age exhibited significant influence on ''Kinnow'' mandarin fruit quality as fruit from young trees (3-years-old) showed inferior fruit quality. Moreover, rind of fruit from young trees had lower macro- (N, P and Ca) and higher micro-nutrient (Mn and Fe) concentrations. In young trees, macronutrients (P and Ca) and micronutrients (Cu, Mn, Fe and Zn) showed a negative correlation with fruit rind thickness. Large sized fruit from all tree age groups exhibited poor quality. Among PGRs, autumn application of 2, 4-D (10 ppm) to young ''Kinnow'' mandarin trees significantly improved TA and TSS:TA ratio. Spring application of cytokinin especially kinetin, among nutrients SSP and CAN and among growth stimulator Wokozim (PGRs and nutrient solution) positively affected fruit physical (rind thickness, total seed, juice, rind and rag mass) and biochemical (TSS, reducing sugars, TA and AA) quality parameters (although some seasonal variations also exist) thus showing their potential for improving fruit quality of young ''Kinnow'' mandarin orchards. An improvement in fruit quality of young orchards (3- 6 years) can help extend the productive window of ''Kinnow'' mandarin orchards.