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Effects of Green Silver Nano Particles on the Resistence Gram Negavtive Bacteria

Thesis Info

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Author

Sabira Parveen

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=141

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720976834

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Escherichia coli and Salmonella typhimurium are the type of gram negative bacteria that can cause different healthcare associated infections. Silver nanoparticles are considered to be used in various applications against bacteria that are opposed to to common antibiotics or even multidrug resistant bacteria as the E. coli and S. typhimurium. In this study, the facile methodology is used for the synthesis of tetracycline capped AgNPs as reducing and capping agent. The formation of the AgNPs was examined by UV?Vis spectroscopy. This characterization confirmed the formation of AgNPs by exciting the surface Plasmon resonance at 407 nm. The Fourier Transform Infrared Spectroscopy (FT-IR) and X-Ray Diffraction (XRD) further inveterate the face centered cubic structure in pure phase. This work was also evaluated the antibacterial activity of synthesized silver nanoparticles against examined bacterial strains as E. coli and S. typhimurium resistant to large number of antibiotics. Regarding antibacterial susceptibility tests, studied bacterial strains showed great sensitivity for synthesized silver nanoparticles. These activities were dose dependent when compared to tetracycline. By using well diffusion method a considerable Zone of Inhibition (ZOI) towards bacterial strains were observed. This high antibacterial potential can be utilized against others lethal pathological bacterial strain for future pharmaceutical products. Moreover, these findings could also pave the way for further investigations such as against sewerage water and waste material reduction.
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