Present study considered to observe the influence of varying dietary protein levels (30%, 35% crude protein and control diet) on proximate body composition of rohu (Labeorohita) from Government Fish Hatchery Multan. The experiment conducted for 2 months trail from October to November in 2017. At the start and end of the experiment-,the whole body composition of fish analyzed. Stocking density was 1000 per acre. In the whole, wet body, weight of Labeorohita the mean percentages for water, fat, protein and ash contentswere 76.86, 2.91, 16.89 and 3.33% respectivelyin this study in all treatments. Higher average protein, fat, ash percentage and minimum water percentage observed with increasing the crude protein in artificial fed. The highest percentage of protein (18.48%) and lowest percentage of water (75.46%) was observed in treatment 3 in which fish were fed with 35% crude protein in artificial diet. Percentage of fat and ash observed in treatment 2 was 3.18% and 2.87% respectively. In treatment 1, observed average percentage of water, fat, ash and protein was 78.71%, 1.98%, 3.24% and 16.07% respectively. Maximum water percentage was observed in treatment 1(30% crude protein in diet) and percentage of protein, fat and ash was low. In treatment2 in which fish were fed with 35% crude protein in artificial diet, average percentage of water, fat, ash and protein was 76.41, 3.58, 3.88 and 16.13% respectively. This estimated that with the increase of protein level in diet, fish highly grow in size. Body composition of fish effected with varying protein levels in artificial diet. The information of these parameters helps to select Labeorohitaas major diet because it contains higher protein content and is suitable for human consumption. This processused to find out the nutritional values of this fish through proximate analysis of body constituents of fish and find out the optimum dietary protein level of fish feed at which fish give maximum nutritional value.