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Home > Identification of New Anti-Glaucoma Agents With Emphasis on the Inhibition of Carbonic Anhydrase Enzyme

Identification of New Anti-Glaucoma Agents With Emphasis on the Inhibition of Carbonic Anhydrase Enzyme

Thesis Info

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Author

Shama Qaiser

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=160

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720982035

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Carbonic anhydrase is metal-containing enzyme that contains Zn and involves the carboxylation of water into carbonic acid, which normally converts into bicarbonate and protons Carbonic anhydrase inhibitors (CAIs) are utilized to lower the intraocular pressure (IOP) in glaucoma condition and also decrease the inflammation associated with increasing ocular hemodynamics, and ocular apoptosis. During the course of research period, a number of synthesized compounds were analyzed by bovine carbonic anhydrase-II (bCA-II) for development of new inhibitors. For this purpose high throughput biochemical mechanism based assay was employed for the identification of new inhibitors of CA-II enzyme. Spectra max-340 and M2 spectrophotometer from Molecular Devices (USA) were used to measure the inhibition potential through absorbance. Out of numerous classes of compound only schiff bases of triazole, tosylhydrazone (sulfonohydrazone), benzylidene and thiourea derivatives, were found to be good inhibitors of CA-II. The identified inhibitors were subjected to IC50 determination by serial dilution. The Ez-fit enzyme kinetics soft were employed to calculate the IC50 values from the % inhibition. The benzylidene derivatives exhibited. IC50 values in the range of 52.68 ? 0.47 to 348.57 ? 3.32 ?M. Schiff Bases of triazole derivatives have shown IC50 values between 98.23 ? 1.60 to 285.87 ? 2.67 ?M. While thiourea derivative exhibit excellent inhibition potential which illustrated IC50 values in the range of 1.90 ? 1.30 to 25.90 ? 2.05 ?M when compared with the standard inhibitor acetazolamide IC50 = 0.13 ? 0.05. Among all the compounds thiourea derivatives can serve as lead compounds for the identification of new inhibitors of CA-II.
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مولانا عبدالمالک جامعی مدنی

آہ! مولانا عبدالملک جامعی مدنی
(مولانا افتخار فریدی)
یہ خبر انتہائی رنج اور صدمہ کے ساتھ سنی گئی کہ مہاجر مدینہ مولانا عبدالملک جامعی، مدینہ منورہ میں انتقال فرماگئے۔ ان کی ذات گرامی گوناگوں خصوصیات کی حامل تھی۔ وہ جامعہ ملیہ اسلامیہ کے سابقین اولین میں تھے اور اس کے کئی شعبے ان کی صلاحیتوں سے پروان چڑھے۔ مولانا، مولانا محمد علی جوہرؔ کے فدائی اور خادم اور سابق صدر جمہوریہ ڈاکٹر ذاکر حسین کے محبوب شاگردوں میں سے تھے، حق بات کہنے میں کبھی ان کو تامل نہیں ہوتا تھا۔ اپنے استاد ذاکر صاحب سے بھی اس وقت کے امر کی ایک بات کہی تھی جس سے ذاکر صاحب رات بھر روتے رہے۔
مولانا عبدالملک کا بچپن اپنے والد منشی عبدالقیوم صاحب کیساتھ جو مشہور خطاط تھے بجنور میں گزرا جہاں سے مشہور زمانہ اخبار ’’مدینہ‘‘ نکلتا تھا۔ یہاں وہ کئی عبقری صحافیوں اور ہمدرد ان ملت کی خاص شفقتوں کے مورد رہے۔ ۱؂ وہیں سے مولانا نے تحریک خلافت میں بھی حصہ لیا، مولانا کے والد محترم نے مولانا ابوالکلام آزاد کے ساتھ کلکتہ میں رہ کر ’’ترجمان القرآن‘‘ کی کتابت بہت ہی بے کسی کے عالم میں کی اور اسے مدینہ پریس بجنور میں طبع کرایا۔ دوسرا ایڈیشن لاہور میں طبع کرایا اور دونوں کا منافع مولانا آزاد کو پہنچایا۔
مولانا عبدالملک صاحب، مولانا محمد علی جوہرؒ کے فدائی اور عاشق تھے اور ساری ایمان افروز تربیت انہیں سے حاصل کی تھی۔ فرمایا کرتے تھے مولانا محمد علی بیسویں صدی کے مرد مومن ہیں۔ ان کا تعلق مولانا کے خاندان کے ہرہر فرد سے تھا۔ کراچی میں بھی ان کے نواسوں سے بڑی محبت کیا کرتے تھے۔
مولانا مرحوم نے تقریباً ۵۰ سال مدینہ منورہ میں قیام فرمایا اور حفظ قرآن کا نظام چلاتے رہے۔ مولانا کی زندگی بہت ہی بے کسی اور...

Comparative Effect Of Tetrapack Juices And Fresh Fruit Juices On Blood Glucose Level Among Healthy Individuals Comparative Effect of Juices on Blood Glucose Level

Uses of tetra pack juices enhances the risk of diabetes and obesity. People were unaware of impact of fresh fruit juices on health. Objective: To compare the effect of tetra pack juices and fresh fruit juices on blood glucoselevels among healthy individuals Methods: 10 participants were selected using an internationalstandard Glycemic Index (GI) test protocol. After getting their fasting blood sample theywereinstructed to consume all the juice served in a period of 5 min. Further blood samples were takenat different intervals of time that is 0, 30, 60, 90, 120 and 150 minutes after consumption. Participants wereremained sedentaryduring each session. Blood was obtained by finger-prick and tested by the glucometer. Results: The glycemic index of Apple juice Nestle has the glycemic index of 95.87, Orangejuice Nestle has the GI value 93.78, Apple juice fresh has the GI value of 92.17 and Orangejuice fresh has the glycemic index value of 99.07, respectively Conclusions: Detailed study of glycemic index of tetrapack juices (Nestle Apple and Orangejuice) and fresh fruit juices (Apple and Orange juice) showed similar impact on the blood glucose level of healthy individual. In the study, both types of juices were found to be equally hyperglycemic (GI 70+) causing a fast rise in blood-sugarlevels, hence should not be given to diabetic patients

Threats to Pakistans Nuclear Weapons: Myth or Reality

Ever since Pakistan carried out its nuclear tests in 1998, the international community has voiced grave concerns over the prospects of Pakistan’s nuclear weapons or fissile material falling into the hands of non-state actors or terrorists. The threat of nuclear terrorism has gained exclusive attention after the events of September 11, 2001, with concerns that terrorists could create even greater destruction if they had gained possession and know-how of nuclear materials and technology. Today nuclear security has become a leading issue of concern in contemporary international politics. Various academics as well as policy analysts have expressed their apprehensions regarding the security of Pakistan’s nuclear programme. So many hypothetical scenarios are being projected in the Western media, portraying a dramatically chaotic situation about Pakistan’s nuclear assets. Since the international community is facing serious challenges regarding nuclear insecurity, any single nuclear incident by a non-state actor may disturb global peace. Elaborating on the serious security threats to Pakistan’s nuclear weapons, Western security analysts and policy makers have pointed out a potential threat from radical militants and their sympathizers in the civil and military organizations. Additionally, it is a fact that terrorist attacks on military installations, including suicide bombings, have been observed in the last few years, all heightening the perception that Pakistan’s nuclear weapons are not secure. Several suppositious situations have been developed by Western analysts in this context; such as, terrorists attacking Pakistan’s nuclear facilities, controlling its nuclear weapons, accidental or unauthorized use of nuclear weapons and questions on nuclear weapons’ security when military installations are under attack. The other side of picture presents a highly professional and confident Pakistan with state of the art safeguards and highly evolved personnel check mechanism involved in its nuclear security protocol. Pakistan’s nuclear establishment has always strongly rejected such claims by media and policy institutes. Pakistan’s nuclear weapons’ safety and security system is as much advanced and robust as the infrastructure of any other nuclear weapon state. International concerns regarding Pakistan’s nuclear weapon programme are based on hypothetical scenarios that have little chance of materialization. This study is an attempt to conduct an in-depth evaluation of the international concerns cultivated through media, Pakistan’s response and the safety measures taken by Pakistan. It is an overall assessment of the issues related to nuclear safety and security of Pakistan’s nuclear weapons programme. The study suggests that Pakistan needs to improve and strengthen its nuclear diplomacy through effective global representation with special focus on countering negative propaganda, which would be the best way to mitigate international concerns about the safety and security of Pakistan’s nuclear assets.