Search or add a thesis

Advanced Search (Beta)
Home > Pathological and Comparative Analysis of Newcastle Disease Virus of Sub-Genotypes Vi G and Vi M in Pigeons

Pathological and Comparative Analysis of Newcastle Disease Virus of Sub-Genotypes Vi G and Vi M in Pigeons

Thesis Info

Access Option

External Link

Author

Safa Ather

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=163

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720983008

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


Newcastle disease viruses (NDV) cause infection in avian species including both poultry and non-poultry worldwide. It is an extremely contagious disease also affecting vaccinated birds. Newcastle disease (ND) is endemic in Pakistan, viruses of sub-genotype VIIi in genotype VII, and sub-genotype VIg and Vim in genotypes VI of class II is continually effecting the pigeons and poultry production facilities. The identification and characterization of these viruses in poultry and non-poultry species is necessary in order to create realistic strategies for preventing and controlling NDV outbreaks. Genotype VI are also termed as pigeon paramyxoviryus-1, are frequently isolated and cause disease from the columbidae family. In this study, two PPMV-1 strains isolated from outbreaks in Lahore district were characterized by pathotypically, genotypically and detailed clinicopathologic assessment. The intracerebral pathogenicity index (ICPI) values for pigeon/Pak/Lahore/SA_1/2017 and pigeon/Pak/Lahore/SA_2/2017 were 1.51 and 1.52 respectively. The complete genome analysis based on full F gene coding sequences of two pigeon origin isolates from Lahore in 2017, along with previously published NDV strains available in GenBank was conducted. Analysis of F gene showed clustering of the isolates with genotype VI g and VI m. The genetic distance and phylogenetic analysis showed comparison with class II genotypes including vaccine strains. Both the strains were up to 10% genetically distant from LaSota (genotype II) isolate. Additionally, when compared for nucleotide and deduced amino acid sequence comparison several mutations were observed. The analysis of F and HN gene functional domains and receptor sites displayed conserved and mutated sequences. Results of genome analysis suggested that PPMV-1 isolates had antigenic variations from vaccine strain LaSota. For histopathological examination pigeon groups were challenged with pi/Lhr/SA_1/17 and pi/Lhr/SA_2/17 strains. Although severe clinical signs were not observed but microscopic results revealed lesions in multiple tissues from selected organs. Though limited data is available in Pakistan, this study will assist in introducing new control strategies and preventive measures for PPMV-1 outbreaks.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

(اچھائی؍نیکی دا بدلہ

اچھائی؍نیکی دا بدلہ

کسے ملک اتے اک ظالم بادشاہ حکمرانی کر رہیا سی۔ اوہ اپنی رعایا اتے بہت ظلم کردا تے اوس دے دربار وچوں کسے نوں وی انصاف نئیں سی ملدا۔ جو وی اوس دے خلاف بولدا، اوہ اوس نوں جانوں مار دیندا سی۔ کسے نوں اوہ پھاہے لاندا تے کسے نوں بھکھے خون خوار جانوراں اگے سٹ دتا۔ کسے دے ہتھ پیر کٹ دیندا تے کسے دیاں اکھاں کڈھ دیندا۔ اک سپاہی نے بادشاہ دے ظلم دے خلاف آواز چکی تاں بادشاہ نے اوس نوں مارن دا حکم دے دتا۔ اوہ سزا توں بچن لئی اپنے گھروں نسیا تے جنگل وچ جا کے لک گیا۔ بادشاہ نے سپاہیاں نوں جنگل جا کے لبھن تے گرفتار کرن دا حکم دتا۔ سپاہی اوس نوں گرفتار کرن لئی جنگل جاندے نیں۔ پر اگوں اوہناں نوں شیر ملدا اے جو گرج دار آواز وچ بول رہیا سی۔ سپاہی ایہہ ویکھ کے  ڈر جاندے نیں تے اوتھوں واپس بادشاہ کول آ جاندے نیں۔ جدوں سپاہی نے اوہناں نوں واپس جاندے ویکھیا تاں اوہ لکی ہوئی تھاں توں باہر آیا۔ اوہ وی شیر نوں ویکھ کے  بہت خوف زدہ ہوندا اے۔ جدوں اوس غور نال آواز سنی تاں اوس نوں لگیا کہ شیر کسے مصیبت وچ اے۔ سپاہی جدوں شیر دے نیڑے ہویا تاں شیر نے اوس نوں کجھ نہ آکھیا، ہمت کر کے سپاہی شیر دے ہور نیڑے ہویا تاں اوس ویکھیا کہ اک تیر شیر دی لت وچ کھبیا ہویا اے تے تیر لگن پاروں لہولہان اے۔ سپاہی نے ہمت کر کے پہلاں شیر دی لت وچ تیر کڈھیا جس پاروں اوہدی پیڑ کجھ گھٹ گئی۔ مڑ اوس نے اوہدے پیر وچوں کنڈا کڈھیا۔ شیر اوس دی ایس رحمدلی تے انسان دوستی توں بہت متاثر ہویا اوس دے پیر چمے تے لنگر ہندا ہویا جنگل ول...

Relationship Between Organizational Culture and Employee Performance Through Work Stress at the Regional Office of the East Java I Directorate General of Customs

This study aims to analyze the Relationship between Organizational Culture and Employee Performance through Work Stress at the Regional Office of the Directorate General of Customs and Excise, East Java I. This research method is an explanatory research, the analysis unit in this study is employees who work in the Directorate General of Customs and Excise Office. East Java I region, which consists of 80 structural officials, 79 functional officials and 1323 executive staff. The method of collecting research data using a questionnaire. The results showed that organizational culture affects the work stress of employees at the Regional Office of the Directorate General of Customs and Excise, East Java I, this shows that organizational culture is able to increase the work stress of employees of the Regional Office of the Directorate General of Customs and Excise, East Java. Organizational culture influences the performance of the employees of the Regional Office of the Directorate General of Customs and Excise, East Java I, this shows that with a good and appropriate organizational culture, it is able to increase the performance of the employees of the Regional Office of the Directorate General of Customs and Excise, East Java I. New organizational culture that also improves Employee stress, namely the imposition of input into daily work activity reports by employees in the daily logbook through an internet-based application with details on the types of activities, time norms, achievement targets and employee daily problems for all levels of employees, both structural, functional and executive.

Personality and Psychological Contracts: Mediating Roles of Epistemic Curiosity and Rule-Following Behavior With Moderating Role of Uncertainity Avoidance

This study evaluates what and how personality traits (Big Five) of employees form their psychological contracts with respective employers. Therefore, apart from testing the direct relations between personality traits of employees and their psychological contracts towards employers, the present study investigates the ex planatory mechanisms (epistemic curiosity & rule-following behavior) between the Big Five personality traits and dimensions of psychological contracts, which in cludes transactional, relational, balanced and ideological contracts. In addition, the moderating role of individual level uncertainty avoidance is also examined in the relations between personality traits and explanatory mechanisms i.e. epistemic curiosity and rule-following behavior. Trait activation theory provides overarch ing theoretical framework for this study and norms of reciprocity offer additional support. Time-lagged data in three time-lags were collected from 469 respondents employed in various public and private sector organizations across Pakistan. Results indicate that Openness to Experience positively relates to transactional, balanced and ideological contracts and negative relates to relational contracts. Conscientiousness positively relates to transactional contracts and negatively re lates with relational, balanced and ideological contracts. The other personality dimension Extraversion positively relates to relational contracts and ideological contracts whereas it negatively relates to transactional contracts. Similarly, Agree ableness is found positively related with relational contracts and negatively with transactional and ideological contracts. Finally, Neuroticism negatively relates with all types of psychological contracts. Regarding explanatory paths, epistemic curiosity and rule-following behavior ex plain the relations between specific personality traits and psychological contracts as epistemic curiosity explains the relations between Openness to Experience, Con scientiousness and Extraversion and psychological contract types. While in case of rule-following behavior, majority of the hypotheses stand accepted, except for balanced contracts. In case of moderation, the results show that uncertainty avoid ance weakens relationship between Openness to Experience, Conscientiousness and x Extraversion and epistemic curiosity. The results regarding moderating role of uncertainty avoidance in the relations between Conscientiousness, Agreeableness, Neuroticism and rule-following behavior could only be partially claimed due to the fact that uncertainty avoidance was not found affecting the relation between Con scientiousness and rule-following behavior. Overall directions of buffering effects were somehow mixed as compared to expectations. These results have theoretical as well as practical significance. The study makes theoretical contribution in psychological contracts body of knowledge by adding two new explanatory mechanisms to elucidate the reasons why people with certain personality traits form different types of psychological contracts and what affect individual level uncertainty avoidance has on these relations. The practitioners can also benefit from these finding as they can ensure better expectations management and personality-job fit.