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Home > Effects of Erythromycin Capped Silver Nanoparticles on Salmonella Typhimurium & Aspergillus Niger

Effects of Erythromycin Capped Silver Nanoparticles on Salmonella Typhimurium & Aspergillus Niger

Thesis Info

Access Option

External Link

Author

Qasim Ali

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2017

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=169

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720984793

Similar


From the past few decades, multiple drug resistivity problems rise day by day. Drugs become resistant over microorganisms like bacteria,fungi, algae etc. To overcome such kinds of resistivity problems researchers have adopted many new ways and methodologies. From these methodologies, one way is to cap nanoparticles with different drugs which may replace conventional antibiotics with these stabilized drugs. Erythromycin stabilized silvernanoparticles (Ery-Ag(0) NPs)werecombined by using AgNO 3 with the assistance of erythromycin by using a facile method. The synthesized(Ery-Ag (0) NPs) wasobserved by UV-Vis absorption spectroscopy, which inveterate the formation of (Ery-Ag(0) NPs)by stimulating the characteristic surface Plasmon absorption maxima at 400-420nm. While the confirmation of bonding of specific drug with silver metal exhibited, the formation of silver nanoparticles was verified by FTIR.X-ray powder diffraction (XRD) confirmed the crystalline nature of synthesized Ag (0) NPs. The standard condition employed for the optimization of this experiment. The effects of (Ery-Ag(0) NPs) were checked on Salmonella typhimurium?(S. typhimurium)and?Aspergillus niger (A. niger).Well diffusion method was used to check the antimicrobial activity on S. typhimurium?and?A. niger, and inhibition zone was in mm to check capability of the capped (Ery-Ag(0) NPs). The results were compared with standard erythromycin. The antibacterial and antifungal results revealedthat newly synthesized (Ery-Ag(0) NPs) had aremarkable activity against S. typhimurium and?A. niger.The results could pave the new ways against multiple drug resistance. The resistance problems caused by conventional drugs and microbial activities by reduction or degradation methods show remarkable and efficient outcomes.
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المبحث السادس: بروين شاکر وفکرتها عن العشق

المبحث السادس: بروين شاکر وفکرتها عن العشق

 یوجد في أشعار بروین شاکر تجربۃ ذاتیۃ لحبھا وعشقھا، فقد کتبت بعض القصائد من تجربتھا الخاصۃ وحبھا القدیم ولکن کان لھا القدرۃ في أن تبقی السر سراً وکانت طریقۃ بیانھا مھذّبة لا تفشي أسرار حُبّھا۔

 فقد کتبت بروین قادر آغا[1] عن بدایۃ حب الشاعرۃ وعشقھا الأول۔ قد أعجبت الشاعرۃ بشاب، وکان موظفًا حکوميًا فشارکتہ في أحلامھا وآمالھا، ولکن ذلک لم یکن رغبۃ الشاب فھو کان لا یُرید الزواج منھا لأنہ کان یختلف عنھا في النسب والحسب وکان یختلف عنھا في الفرقہ الدینیۃ، بأنہ کان من أھل السّنہ والشاعرۃ کانت من أھل الشیعۃ۔ فرفض ذلک الشاب الزواج، فکان ھذا أمرٌ صعبٌ للشاعرۃ لأنھا کانت تحب ذلک الشاب، ولکن بعد فترۃ أحسّ الشاب بالندم علی ما فعل مع الشاعرۃ فرجع لھا وأراد الزواج منھا، فأحست الشاعرۃ بالفرح ولکن والدیھا لم یرضوا بذلک الشاب والزواج منہ، فأحست الشاعرۃ بالحزن مرۃً أخری وتقطّع قلبھا من الألم والیأس، وعاشت أحزانھا معھا إلی أن تکوّن عندھا قابلیۃ علی إظھار مشاعرھا وأحزانھا۔ وکتبت الشاعرۃ الکثیر من أشعارھا توضح وحدتھا وألمھا۔

 ثم جاء لھا خاطب آخر، فوافق والداھا علی زواجھا فتزوجت الشاعرۃ من نصیر علي وأنجبت منہ إبناً أسمّتہ (مراد) وعندما تزوجت الشاعرۃ أحست بالفرح ولذۃ العشق في أول أیام زواجھا، ولکن بعد مدۃ من الزمن حصلت خلافات بین بروین شاکر وزوجھا وحصل البعد بین الزوجین، فأحست الشاعرۃ بالیأس والحزن والوحدۃ وفراق الزوج فقامت الشاعرۃ بإظھار مشاعرھا وآلامھا وأحزانھا في قصائدھا بشكل صريح ۔

 

 



[1] بروین قادر آغا عمۃ الشاعرۃ تعیش في إسلام آباد، وھي أیضاً شاعرۃ رائعۃ۔

عہد نبوی میں ذرائع کے اسالیب عصر حاضر کے نوجوانوں کے لیے مشعل راہ

Since from the beginning of humanity means of communication have always been an essential need for mankind. To convey the message and to find means to communicate and express one’s thought one needs a mean to transmit the information to others. That is called communication. With the passage of time and advancement communication means also took modern shape and became advance. Islam as a complete code of life, guides humanity in the all fields of sociology, economic, politics, including mass communication. Allah the Almighty sent messengers and Prophets for the guidance of people. So they served the humanity in different periods of time in different areas. Ḥaẓrat Muḥammad (ﷺ) the last prophet of Allah used these means of communication for the prevalence and preaching of Islam, and left behind a remarkable legacy in the field of mass communication for the guidance till dooms day. Where there have been great changes in other fields and professions of life in the advanced world of contemporary era there had become a revolutionary change in the field of media. News all over the world spread in seconds. Media which is the strongest tool to approach people, but sorry to say that it is detracted badly by prevailing wrong values, vulgarity, jealousy and selfishness. There is a dire need to change the direction of noble that so (صلى الله عليه وسلم) Muhammad Prophet of methodology the towards media values like piousness, self-sacrifices, brotherhood and cooperation should be developed in people and for this purpose youth can play a pivotal and effective role in the field of mass communication. Eyes are looking towards youth of contemporary era to step forward by following our Holy contemporary the how that attempt humble a is article This. (صلى الله عليه وسلم) Prophet youth can play its role by using means of communication by taking guidance from Prophetic Era to lead media towards right direction.

Prevalence of Fusarium Toxins: Ecology and Implications for the Control of Trichothecenes and Zearalenone Contamination in Cereal Grains

Fungal infection of cereals grains is a problem of public health concern due to the possible risk of mycotoxins contamination. The present study investigated the prevalence of Fusarium species and their mycotoxins zearalenone (ZON), HT-2, T-2, deoxynivalenol (DON), 3-ac DON and 15-ac DON contamination in maize, wheat and rice samples from five agroecological zones i.e. zone-A, B, C, D and E of Khyber Pakhtunkhwa Province of Pakistan in spring (March-May), summer (June-August), autumn (September-November) and winter (December-February) seasons of the year 2015-16. In addition, different physical and chemical strategies were adopted to control Fusarium strains and their associated mycotoxins in maize, wheat and rice grains during storage. The mycological analysis indicated that maize, wheat and rice samples from zone-B in autumn season contained the highest total fungal viable counts i.e. 11.50×103, 3×103 and 0.8×103 CFUs/g, respectively whereas the lowest total fungal counts i.e. 6.50×103, 1 ×103, 0.22×103 CFUs/g, respectively were recorded in the samples from zone-D in spring season. Similar pattern of results was noted for total Fusaria counts in maize, wheat and rice samples. The analysis of maize, wheat and rice samples for the identification of mycotoxigenic Fusaria showed the presence of seven species which were F. graminearum, F. langsethiae, F. poae, F. culmorum, F. crookwellense, F. sporotrichioides and F. equiseti. The species showed significant (p<0.05) variation in their occurrence frequencies with respect to season, agroecological zone and cereal type. However, the overall dominant toxigenic Fusarium species in maize and rice was found to be F. langsethiae and that in wheat was F. graminearum. The data regarding mycotoxin contamination of maize, wheat and rice samples revealed that both the zones and seasons alone and in combination significantly (p<0.05) affected the mycotoxins contents of these cereals. The average value of ZON contents ranged from 610.40 to 1280.42 µg/kg in maize, 45.22 to 120.32 µg/kg in wheat and 40 to 45 µg/kg in rice. The HT-2 contents of maize samples ranged from 140.35 to 245.62 µg/kg; the lowest in the sample from zone-E in spring season and highest in the samples from zone-B in winter season. Similarly, the HT-2 content of wheat samples expressed that the highest content (65.46 µg/kg) was present in the samples from zone-B in autumn season whereas the lowest amount (45.18 µg/kg) was recorded in the samples from zone-E in spring season. The rice samples showed highest HT-2 content (35.23 µg/kg) in the autumn season of zone-B and the lowest (23.25 µg/kg) in the spring season of zone-E. The T-2 contents of maize ranged from 130.50 µg/kg in samples from zone-D in spring season to 530.55 µg/kg in samples from zoneB in the autumn season. The wheat samples expressed maximum T-2 contents (60.55 µg/kg) in the autumn season of zone-B and the lower most (35.24 µg/kg) was recorded in the spring season of zone-E. The rice samples generally showed little T-2 contents as compared to maize and wheat. The analysis of maize samples for DON contents showed the highest concentration (1570.48 µg/kg) in the samples of zone-B in autumn season while, the lowermost content (190.52 µg/kg) was perceived in the spring samples of zone-D. Likewise, the wheat samples showed the least DON contents (50.35 µg/kg) in the spring season of zone-C and the highest (110.15 µg/kg) in the samples from zone-B in autumn season. Similarly, rice samples from zone-B expressed the highest DON contents (40.37 µg/kg) in autumn while the lowest contents were recorded in the samples from zone-A in spring season. Data regarding 3-ac DON content of maize samples revealed that its contents were significantly (p<0.05) higher in zone-B during autumn season. The wheat samples from zone-C, D and E showed no detectable concentration of 3-ac DON whereas the samples from zone-A and B showed the contents ranging from 15.28 to 35.25 µg/kg. Similar pattern of results was noted for rice samples. The 15-ac DON contents were detected only is the samples from zone-A (maize and rice), zone-B (maize, wheat and rice) and zone-E (wheat) whereas no detectable level of it was analyzed in the samples from other zones during the four seasons of the year. The application of γ-radiation at dose rates of 5 and 10kGy on naturally contaminated maize, wheat and rice samples showed that total fungal counts, total Fusaria counts, and Fusarium mycotoxins were significantly reduced at 10kGy irradiations. Similarly, the samples of naturally contaminated maize, wheat and rice were subjected to dry heat treatment at 150 and 180°C. The total viable fungal counts and total viable Fusaria counts of all the three cereals were completely eliminated at 150 and 180 °C and the mycotoxins were substantially reduced. The control (no heat treated) maize samples contained 935.55, 308.30, 230.73, 1451.68, 362.11 and 156.71 µg/kg of ZON, T-2, HT-2, DON, 3-ac DON, and 15-ac DON mycotoxins, respectively. These mycotoxins were reduced to 186.57, 113.88, 37.51, 23.34, 37.86 and 39.87 µg/kg, respectively after heat treatment at 180°C. Similar results were noted for wheat and rice samples. Likewise, the total fungal count and total Fusaria counts were significantly affected by calcium propionate preservative, aw and storage time. The total fungal count in maize was maximum with no preservative (12.25×103CFUs/g) at 0.95 a w at 45 day of incubation period, whereas the fungal population decreased significantly to 7.2×103CFUs/g at 0.95 aw, 45day storage time and 1% calcium propionate. Similarly, the total fungal count, total Fusarium count for wheat and rice samples was also significantly controlled by calcium propionate as preservative at 1% concentration at reduced a w level. The mycotoxins ZON, HT-2, T-2, DON, 3-ac DON and 15-ac DON contents of maize, wheat and rice were significantly (p<0.05) affected by aw, storage time and preservative concentration. Generally, the concentration of all these mycotoxins in maize, wheat and rice samples increased with increasing aw level and storage time whereas a reversal of the trend was noted with increasing concentration of calcium propionate preservative. It was concluded from the study that the major cereals i.e. maize, wheat and rice of Khyber Pakhtunkhwa were contaminated with Fusarium mycotoxins whose severity was dependent on seasonal variation and geographical locations. Therefore, it is recommended to adopt suitable prevention and control measures to tackle mycotoxins contamination problem in cereals on priority basis.