Poultry feed samples (Chick feed, Broiler feed, Layer feed and Grower feed) collected from different areas of Pakistan were evaluated for bacterial and fungal contamination. Total Viable count, Total coliform count, Total Staphylococcus Count and Total Salmonella and Shigella count was done. Isolated colonies were identified by Morphological, Biochemical (Gram stain, Catalase, Oxidase and Sugar Fermentation Test) and Molecular (16S rRNA gene) means. Bacteria belonging to seven different genera were isolated. Percentage of positive samples found for each isolate was: Bacillus (100%), Salmonella (10%), Shigella (15%), Staphylococcus (100%), Enterobacter cloacae (5%), Escherichia (90%) and Ralstonia (15%) were isolated from feed samples. The highest contamination was found in chick feed and Total Viable Count (TVC) obtained in chick feed (S1) was 9.1?108CFU/g. Total coliform count (TCC) was found in the range of 0 - 3?108 CFU/g. Total Salmonella count is found to be in the range of 3.5x106 - 3.52?106 CFU/g and total Shigella count was found to be 1.32?105- 6?105 CFU/g.Highest Staphylococcus count was 4.9x108 CFU/g. Isolated fungi were identified as Aspergillus niger and Aspergillus flavus.