CYP11B2 gene is located over the upper layer of the kidney. It produces aldosterone synthase enzyme and thereby has an essential role to balance salt and mineral level in the body. Any SNP (single nucleotide polymorphism) mutation in this gene can dis-regulate the production of aldosterone hormone in the body which may lead to many diseases including hypertension and cardiac diseases. To control the excess production of this aldosterone an inhibitor ?Fadrozole? is being used by scientists; the Fadrozole associates with an active site cavity of CYP11B2. This study has been divided into two portions; in the first phase, the four computational tools (SIFT, Polyphen-2, I-Mutant, ConSurf) were used to identify 29 deleterious SNPs out of 1600 CYP11B2 SNPs. In the second phase, five residues (R448G, R141P, W260R, F130S, and F445S) were identified in the active site cavity (out of 29 deleterious CYP11B2 SNPs) at the distance of 5Ao using PyMol. Dynamic simulation and binding free energy calculation techniques were applied to determine the effect of these mutations on the CYP11B2-Fadrozole compound. The results showed that Fadrozole binding becomes stronger with CYP11B2 with these mutations which proved the efficiency of this drug inhibitor with these highly damaging mutations. This study narrows down the large dataset of CYP11B2 SNPs and identifies 29 deleterious SNPs as well as it validate the fadrozole inhibitor efficiency for cavity residues