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In-Silico Analysis of Nssnps Associated With Cyp11b2 Gene

Thesis Info

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Author

Anam Arooj

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=177

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720986192

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CYP11B2 gene is located over the upper layer of the kidney. It produces aldosterone synthase enzyme and thereby has an essential role to balance salt and mineral level in the body. Any SNP (single nucleotide polymorphism) mutation in this gene can dis-regulate the production of aldosterone hormone in the body which may lead to many diseases including hypertension and cardiac diseases. To control the excess production of this aldosterone an inhibitor ?Fadrozole? is being used by scientists; the Fadrozole associates with an active site cavity of CYP11B2. This study has been divided into two portions; in the first phase, the four computational tools (SIFT, Polyphen-2, I-Mutant, ConSurf) were used to identify 29 deleterious SNPs out of 1600 CYP11B2 SNPs. In the second phase, five residues (R448G, R141P, W260R, F130S, and F445S) were identified in the active site cavity (out of 29 deleterious CYP11B2 SNPs) at the distance of 5Ao using PyMol. Dynamic simulation and binding free energy calculation techniques were applied to determine the effect of these mutations on the CYP11B2-Fadrozole compound. The results showed that Fadrozole binding becomes stronger with CYP11B2 with these mutations which proved the efficiency of this drug inhibitor with these highly damaging mutations. This study narrows down the large dataset of CYP11B2 SNPs and identifies 29 deleterious SNPs as well as it validate the fadrozole inhibitor efficiency for cavity residues
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کراچی کا شیر

کراچی کا شیر

                                                                                                                مرزا مقبول احمد

کراچی کا شیر مگر جس کی بہادری اور جرات کے قصے پنجاب کی جیلوں شاہی قلعوں کے عقوبت خانوں میں لکھے ہوئے ہیں ۔دبلا پتلا سا خوبصورت جوان مرزا مقبول اپنے وزن سے بھاری بیڑیاں پہنے چھن چھن کرتا ہنستا مسکراتا کوٹ لکھپت جیل کے احاطے میں دندناتا پھرتا آج بھی نظروں میں گھوم جا تا ہے تو اس کے لیے ڈھیروں پیار دل میں بھر جا تا ہے ۔عارف لوہار کے چمٹے کی طرح بیڑیا ں بجاتے گنگناتے ہوئے :

جانے کیوں لوگ محبت کیا کرتے ہیں

دل کے بدلے درد دل لیا کرتے ہیں

گاتا تو محسوس ہو تا ہے کہ محبت کی کوئی ادھوری داستان جیل سے باہر چھوڑ آ یا ہے ۔مرزا مقبول استاد مسرور احسن ہو غلام حسین چانڈیو یا مصطفی میمن ہو یا دیگر ساتھی اپنی گہری یادیں پنجاب چھوڑ آئے ہیں، جنہیں دوست آج بھی یاد کرتے ہیں ہم بھول بھی کیسے سکتے ہیں اپنے انمول ساتھیوں کو ۔سندھ والو ان کی قدر کرو جس عزت و احترام کے قابل یہ لوگ ہیں انہیں ضرور دینا چاہیے ۔ایسے ہیروز روزر وز پیدا نہیں ہوتے ۔

 

 

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