Fingerprint (Dermatoglyphics/dactylography) is an easy and secure tool for the identification of a person. It provides most reliable and effective data that can be used for different purpose. It is a very efficient technique that depends upon two major aspects. The ridges of fingerprints are formed during the fetal period and remain same throughout the life of an individual; the other one is that two persons cannot have finger prints alike in this world. This study showed the relationship of fingerprint patterns with blood group (ABObloodgroup)andgenderofanindividual.Inthisstudy,200individualsof different populations were selected from Sialkot region, in the Punjab Pakistan, with 100 males and 100 females. These individuals belonged to different age groups. The distribution of different pattern of fingerprints showed some peculiarities to blood group. Fingerprints are categorized into Loops, Whorl, Arch and Composite types. Blood group B existed predominant follower by A, AB and O in a population. It was also found that dispersion of different types of blood groups and genders has no association. Loop and whorl pattern were more common in males than females while arches and composite patternsweremorecommoninfemalesthaninmales.Thesignificantassociation between gender and fingerprint patterns had been found. It was observed that an association also exists between dispersion of different types of finger prints and blood groups. Blood group B had higher frequency of Loops and Whorls type fingerprints, where as O type blood group was noticed to have least frequency of Arches. This study may help in developing the basic data for the population of Sialkot and would help in forensics in future.