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Home > Molecular Characterization and Seroprevalence of H9 Avian Influenza in National Zoo and Live Birds Market Lahore District and Azad Kashmir

Molecular Characterization and Seroprevalence of H9 Avian Influenza in National Zoo and Live Birds Market Lahore District and Azad Kashmir

Thesis Info

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Author

Kashaf Alyas

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=189

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720989206

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Avian influenza virus (AIV) subtype H9N2 is greatly threatening to Pakistan poultry, even with stringent vaccination policies. Subtype H9N2 avian influenza viruses (AIV) continues to cause significant outbreaks in commercial and backyard poultry in Pakistan. Subsequently, subtype H9N2 AIVs are occasionally reported from humans, widespread incidence of H9N2 in poultry and non-poultry avian species executes a great risk for public health. Until now, the genetic evolution of H9N2 viruses in various host organisms in Pakistan has not been thoroughly investigated. However, this is the primarily report of isolation of low pathogenic AIV in non-poultry avian species including ducks, black swan, peacock and pheasants in Pakistan. The study has been carried in National Zoos and live birds market (LBM) situated in Lahore district and commercial and backyard poultry in Kasure, Gujranwala, Sheikhpura and Sialkot districts of Punjab province and Bhimber, Kotli and Mirpur districts of Azad and Jammu Kashmir (AJK). In the present study, twelve low pathogenic avian influenza viruses (LPAIVs) subtype H9N2 isolated from clinically healthy and diseased ducks, geese, black swans, pheasants and peacocks and five from poultry outbreaks during 2016-17 were characterized phylogenetically on the basis of hemagglutinin (HA) and neuraminidase (NA) genes. Phylogenetic studies showed that these H9N2 AIVs belonged to Middle East B genetic group of G1 sub-lineage. All the viruses carried an amino acid substitution Q226L in HA gene in the receptor binding site that contributes to increased replication and virulence in mammals. In the present work, genetically very closely H9N2 viruses isolated from poultry and multiple non-poultry avian species. We demonstrated the existence of epidemiological links between poultry and other avian species kept in captivity. Therefore, these findings suggested the continuing surveillance of poultry and other avian species in this region. This demonstrates an epidemiological link between poultry and other avian species kept in captivity, a fact which must be considered in future H9N2 disease management programs.
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مولاناشبیر احمد عثمانی

علامہ شبیر احمد عثمانی
افسوس کہ آج قلم کو اس ہستی کا ماتم کرنا پڑ رہا ہے جو ساری عمر قوم و ملت کی غم گسار رہی، حضرت مولانا شبیر احمد عثمانی کی وفات کا سانحہ نہ صرف پاکستان بلکہ علمی و مذہبی دنیا کا بڑا حادثہ ہے، و ہ اس دور کے جلیل القدر عالم، متبحر فاضل، نامور خطیب اور صاحبِ بصیرت مدبر تھے، دینی علوم میں ان کا پایہ بہت بلند تھا، ان کی ساری عمر ان کی خدمت میں گذری، برسوں دارالعلوم دیوبند میں ان کا علمی فیض جاری رہا۔ پھر ڈابھیل (سورت) کی مشہور دینی درسگاہ میں چلے گئے اور وہاں کئی سال تک ان کے درس و افاضہ کا سلسلہ قائم رہا۔ قرآن مجید پر ان کا اردو حاشیہ موضح الفرقان اور صحیح مسلم کی ضخیم عربی شرح فتح الملہم ان کا بڑا علمی و دینی کارنامہ ہے، اس کے علاوہ چند چھوٹی چھوٹی تصانیف بھی ان کی یادگارہیں۔
ملکی اور قومی کاموں میں بھی ان کا حصہ رہا ہے، وہ عرصہ تک جمعیۃ العلماء کے شریک کار رہے، پھر پاکستان کی تحریک کے بعد مسلم لیگ میں شامل ہوگئے، اور جمعیۃ علمائے اسلام کے صدر منتخب ہوئے اور پاکستان کی تبلیغ و اشاعت میں بڑی سرگرمی سے حصہ لیا، اس کے چند ممتاز بانیوں میں سے ایک وہ بھی تھے، قیام پاکستان کے بعد کراچی چلے گئے، اور اس کی دستور ساز اسمبلی کے رکن مقرر ہوئے، پاکستان میں ان کی حیثیت مذہبی مشیر کی تھی، اور حکومت کے ارکان پر ان کے علم و عمل، تقویٰ و دیانت، فہم و فراست، اخلاق و سیرت اور استغنا و بے نیازی کا بڑا اثر تھا، اور ان کی ذات سے پاکستان کی مذہبی اصلاح کی بڑی امیدیں وابستہ تھیں، لیکن افسوس موت نے اس کا موقع نہ دیا، اور گذشتہ ۱۳؍...

نظام التعليم المسجدي في الصين

Praise be to Allah and peace and blessings be upon the leader of all the Prophets, upon his descendants, his disciples, and the ones who follow him to the Day of Judgment.     Islam entered China as early as in the first century Hijrah corresponding to the seventh century AD, in the reign of Caliph Othman. From the beginning until current time, ten national minority groups accepted Islam.   Chinese Muslim Community is the second biggest Muslim minority group in the world, but tops the chart when it comes to bearing the hardships for Islam. The Mosque in china not used only for prayer, but also a place for Muslims to learn Islamic knowledge, which has played a significant role in consolidation of faith and alleviating hardships. But unluckily most of foreigner Muslim brothers do not know about Mosque education system in china, that’s why I have chosen this topic to provide basic concept about Mosque education (Madrasa) in china.   Lastly, I hope this small article would be useful and wish the readers might get the most benefits from it. Inshallah!

Comparativegrowthperformanceand Morphologicalvariationsanalysisbetween Two Populationsof Cirrhinus Mrigala from Chenab and Indus Rivers

In Pakistan there exist huge potential for development of aquaculture particularly inland fisheries sector but due to couple of reasons this field could not flourished in Pakistan. Cirrhinus mrigala is extensively cultured major carp among other major carps but there is no documentation concerning the analyses of Cirrhinus mrigala from Chenab River and Indus River for stock identification and conservation purposes. Length-weight and length-length relationships, condition factor, stock structure and morphological heterogeneity of Cirrhinus mrigala from Chenab River and Indus River were studied using traditional morphometric techniques; landmark (Truss) based geometric morphometric analysis and meristic analysis. A total of 248 samples were collected, 124 samples from each river. The higher value of regression coefficient ?b? from Indus river (b = 3.130) than regression coefficient ?b? value form Chenab River (b = 2.9875) indicated that Cirrhinus mrigala in Indus River shows comparatively better growth as compared to Chenab River. Length-weight relationships of Cirrhinus mrigala are suggestive that the wild conditions in Indus River at Taunsa Barrage are much suitable for rearing and stocking purposes instead in Chenab River at Head Muhammad Wala. Fulton?s condition factor (K) for Cirrhinus mrigala in Chenab River has shown a decreasing (negative) trend, while for the Indus River it showed an increasing (positive) trend. The higher evaluated level of Fulton?s condition factor for the population of Cirrhinus mrigala in the Indus River than the Chenab River population symbolizes the superior ecological conditions, foraging conditions, health and hygienic conditions in the Indus River at Taunsa Barrage. Discriminant function analysis on 21 landmark based geometric variables and 11 traditional morphometric variables disclosed that phenotypical variations are predominantly exist as Intra-population variations rather than inter-population variations. Final classification results in Discriminant function analysis, accounts that 67.7% of original grouped cases were correctly classified and 56.5% of cross-validated grouped cases were correctly classified. The equal discriminatory power of the discriminant function I (DFI) centred on discriminant function analysis for the both populations of Cirrhinus mrigala at Chenab River and the Indus River and non-significant (H) value of Kruskal-Wallis test patently represents the absence of statistically significant meristic variations between the two populations. Discriminant functional analysis and meristic analysis are of suggestive that there exists much merger and gene flow between two populations owing to weak geographical isolation between two rivers.