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Home > Production of Biodiesel by Enzymatic Transesterification of Non-Edible Salvadora Persica Pilu Oil and Crude Coconut Oil in a Solvent-Free System

Production of Biodiesel by Enzymatic Transesterification of Non-Edible Salvadora Persica Pilu Oil and Crude Coconut Oil in a Solvent-Free System

Thesis Info

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Author

Azeem Abdul Aziz Budhwani

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=193

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720990457

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Biodiesel is one of the renewable and eco-friendly fuels which can alleviate the concerns associated with fossil such as fluctuating prices and disastrous effects on the environment including global warming. The biofuel is usually produced from transesterification of vegetable oils and consists of alkyl esters. The chemical method of producing biodiesel has several drawbacks while the use of edible vegetable oils for biodiesel raises several concerns such as food vs. fuel crisis. The current work investigated biodiesel production from non-edible Salvadora persica seed oil (SPSO) and crude coconut oil (CCO) catalyzed by Burkholderia cepacia lipase in a solvent-free system. The biodiesel yield produced from these feedstock was compared and the effect of acyl acceptor (ethanol) in different ratios on biofuel production was determined. The fatty acid composition of SPSO and CCO was determined through gas chromatography and their average molecular weight was calculated. Different molar ratios of oil and ethanol were employed in transesterification for biodiesel production at 50?C, 7.0 pH with 50 mg lipase. Thin layer chromatography of oils and biodiesel samples was performed while the percentage yield was determined through gas chromatography. The results showed that medium chain fatty acids make up to 53.88% and 76.89% of the fatty acid content of SPSO and CCO respectively. Both are good candidates for enzymatic transesterification to produce medium chain biodiesel. The species of Salvadora persica (Pilu) has potential of a biodiesel crop. The average molecular weight was calculated as 749.53 g/mol for SPSO and 664.57 g/mol for CCO. Maximum biodiesel yield (around 70%) was obtained at 1:4 oil to ethanol molar ratio from both oils followed by a gradual decline at higher ratios. The gas chromatographic analysis of Salvadora biodiesel at 1:4 molar ratio showed that the yield of individual esters was mostly of medium and long chain fatty acids. In contrast the analysis of coconut biodiesel revealed that it consists mainly of the esters of medium chain fatty acids. Burkholderia cepacia lipase is a promising biocatalyst for production of biodiesel from SPSO and CCO provided the right conditions including optimum oil to ethanol molar ratio. Since free lipase was used in this study, stable yields of biodiesel as well as of individual fatty acid ethyl esters would have been achieved if any form of immobilized lipase would have been used.
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باب اول: قدرتی وسائل کا مطالعہ

قدرتی وسائل کا تعارف

فیروز اللغات میں قدرتی کے لغوی معنی " طبعی، فطری، اصلی، حقیقی، پیدائشی" [1] بیان کئے گئےہیں۔ جبکہ وسائل کا لفظ وسیلہ کی جمع ہے اس لئے فروز اللغات میں وسیلے کے لغوی معنی "وسیلے، واسطے"[2] کے بیان کئے گئے ہیں۔ علمی اردو لغت کے قدرتی کےلغوی معنی "قدرت سے منسوب، فطری، خلقی، پیدائشی، اصلی، ذاتی۔ "[3] بیان کئے گئے ہیں۔ جبکہ اردو لغت میں وسیلہ کے معنی "ذریعہ، واسطہ، سبب"[4] بیان کئے گئے ہیں۔

"البحث اللغوی عند العرب "میں قدرتی وسائل کو بیان کیا گیا ہے:

"المعجم مبوب بحسب ما في الكون كله من آثار في الأرض، وآيات في السماء وبكل ما تحمل الدنيا ويدب فيها من إنسان أو حيوان أو طير أو نبات، وما تحفل به بطنها من معدن، أو ينتأ فوقها من صخر"[5]

 کائنات میں زمین اہم قدرتی وسیلہ ہے جس پر انسان، جانور، پرندے، حیوانات اور دوسری اشیاء پائی جاتی ہیں۔ زمین کا پیٹمعدنیات سے بھرا ہوا ہے جبکہ دوسرے قدرتی وسائل زمین کی سطح پر پھیلی ہوئے ہیں۔ پانی، ہوا، خوراک اور روشنی و حرارت جیسےقدرتی وسائل کرہ ارض پر جانداروں کی حیات و بقا کے لئے بنیادی ضروریاتِ زندگی ہیں۔

 معروف مسلم فلاسفر امام غزالیؒ لکھتے ہیں:

" الأموال إنما تحصل من المعادن والنبات والحيوان"[6]

امام غزالی ؒ کے مطابق انسان کی معاشی ضروریات کرہ ارض پر پائی جانے والے قدرتی وسائل معدنیات، نباتات اور حیوانات سے پوری ہوتی ہیں۔ پس ثابت ہوا کہ دنیا کا پورا معاشی نظام تین بنیادی اور بڑے قدرتی وسائل معدنیات، نباتات اور حیوانات پر انحصار رکھتا ہے۔

بھارت کا ماہر ماحولیات A R Agwᾱn لکھتا ہے:

Demographic Profile Associated With Malnutrition

Worldwide, malnutrition is the severemost health problem leading to the highest rate of disease and mortality among children less than 5 years of age. Objective: To find out the association between malnutrition and demographic profile. Methods: 350 malnourished children were chosen by non-probability convenient sampling technique from Sir Ganga Ram Hospital, Lahore. Children were assessed through pre-tested questionnaire. Data were analyzed by SPSS version 21.0. Results: 45% malnourished children were 1-3 years of age, majority of the children were females (52%), 89% children were from rural areas, 82.6% children were from low socioeconomic status, 54.6% mothers were uneducated, 50% malnourished children were not having their own house, 115 malnourished children were having 3 or more siblings and 89 mothers were having less than one year of pregnancy gap. Conclusions: Low socioeconomic status, illiteracy of mothers, rural area, gap between pregnancy and female gender has been found to be linked with malnutrition in children below 5 years of age.

Effect of Automobile Related Metal Pollution on Plants: A Case Study Along Two Roads Faisalabad to Sargodha and Pindi Bhattian to Lillah in the Punjab, Pakistan

Automobiles release a number of toxic metals into the surrounding environment. They enter human body through food chain and cause many toxic effects. Plants prove good indicators of their existence. In this study five herbaceous plant species (Calotropis procera, Datura alba, Ricinus communis, Parthenium hysterophorus and Cenchrus ciliaris) commonly growing along two roads i.e. Motorway (M-2) and Faisalabad-Sargodha road (FSR) in the Punjab, Pakistan, were collected. Plant and soil samples were collected in all the four seasons (2013-2014) from roadsides. Samples taken 100 m away from roads were designated as control. Lead (Pb), cadmium (Cd), nickel (Ni) and zinc (Zn) metals were analyzed in all the plants and soil samples by ICP-AES (Inductively Coupled Plasma Atomic Emission Spectrophotometer). Carbon (C) and nitrogen (N) contents in plants and soil samples were also measured. Relative plant attributes i.e. photosynthetic pigments (chlorophyll a, b, total chlorophyll contents and carotenoids), gas exchange characters (photosynthetic rate (A), transpiration rate (E), stomatal conductance (gs), internal CO2 concentration (Ci) and water use efficiency (WUE)), total soluble proteins, total free amino acids and total antioxidant activity were studied. Significantly higher concentrations of all the metals were found along roadsides in plants and soils as compared to controls and they clearly showed spatial and temporal variations. In both plants and soil samples, higher contamination of metals was recorded during summer season, while, the least contamination was noticed during winter season. The metals concentrations were obtained in the order Zn > Ni > Pb > Cd. Higher metals, C and N concentrations were recorded along FSR road as compared to M-2. Pull-111 was proved to be the most polluted site. A general reduction in photosynthetic pigments, photosynthetic rate, transpiration rate, stomatal conductance and total soluble proteins were recorded, whereas, increase in internal CO2 concentration, water use efficiency, total free amino acids and total antioxidant activity was observed under metal toxicity. Among plants, Calotropis procera leaves accumulated the highest level of Pb, Cd and Ni, while, Ricinus communis showed tendency to accumulate high quantities of Zn, thus, these plant species can be used as good biomonitors / phytoremediators. The metal contents in plants at most of the sites showed significant positive correlation with traffic density. High level of metals was also found in fuel (petrol and diesel) and soot samples. So, control measures are required to overcome transport sector related pollution which may become severe in forthcoming days.