Hepatitis is a major disease spreading more rapidly in all over Pakistan and its detection is increasing day by day. Knowledge of the distribution of HCV has important clinical implications since the efficacy of current and new therapies. The treatment of chronic hepatitis C virus (HCV) infection has the potential to change significantly over the next few years as therapeutic regimens are rapidly evolving. However, the burden of chronic infection has not been quantified at the global level using the most recent data. Cohort and cross sectional study 534 blood samples randomly selected patients of pulmonary or extrapulmonary tuberculosis (T.B) was calculate utilizing Quantitative Polymerase chain reaction (qPCR). We examined Hepatitis C virus (HCV) by ELISA and confirmation by qPCR. Risk factors estimated and study association among the triple or Co-infection of (HCV), and mycobacterium tuberculosis in dissimilar period of life and groups of patients. Distribution of co-infection was diverse in regions. Decision between the 534 positive T.B patients analysis, 498 (93.2%) patients were negative and 93 (17.41%) were positive for HCV by using the qPCR technique. The transference of HCV was estimated to have take place by and large between ages of 20-40, unmarried, poor and illiterate. Detection rate is high of HCV in males rather than females. There was developed a correlation between co-infection of HCV and tuberculosis patients due to low immunity and body mass index (BMI). We found that HCV can be effect on TB patients. Active tuberculosis is leading to the cause of death living with HCV.There is need Standard operating protocols (SOP?s) for better management of tuberculosis patients.