Poultry industry is facing many challenges one of them is coccidiosis. Seven species of Eimeria are responsible for coccidiosis. These are protozoan parasites in chickens and other animals. A survey was carried out in district Mianwali between July 2017 to July 2018 with the objectives of isolation, prevalence and characterization of Eimeria oocysts. There was no published data related to coccidiosis in this area. For this survey 600 faecal samples were collected. Centrifugal floatation method was used for the examination of faecal samples. Overall prevalence was 32.3%. Young chickens showed more percentage of prevalence as compared to adult ones among the 3 tehsils of districtMianwali. Broiler breed showed more percentage of prevalence as compared to Desi breed. Chicks reared under the intensive management system showed more percentage of prevalence as compared to extensive one. Chicks with poor body condition showed more prevalence of coccidiosis as compared to good body condition. Prevalence of Eimeria species across the district Mianwali were following,in tehsil Mianwali E. tenella 40.44%, E. acervulina 26.96%, E. maxima 20.22, E. necatrix 12.35%. In the tehsil Isakhel prevalence was E. tenella 0. 484%, E. acervulina 0.29%, E. maxima 0.194%, E. necatrix 0.032%. In the tehsil Piplan E. tenella 31.08%,E. acervulina 10.81%, E. maxima 39.18%, %, E. necatrix 18.19%. In age group prevalence was varied amongdistrict Mianwali. It is concluded for control and preventive administration risk factors should be take into account. Coccidiosis can be controlled by using proper medication, avoid from overcrowding and proper sanitary conditions.