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Home > Computational Analysis of Functional Single Nucleotide Polymorphism Associated With Bcl2 Gene

Computational Analysis of Functional Single Nucleotide Polymorphism Associated With Bcl2 Gene

Thesis Info

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Author

Asim Khurshid

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=224

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676720999417

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Most persistent genomic variations are marked as single nucleotide polymorphisms called SNPs, and these are strongly linked to certain disease. Some of these SNPs are known as missense SNPs (nsSNPs) which are residing in the translation region of gene causing an effect on the amino acid composition. Resultantly, these have a deep effect on protein structural and functional aspects. In human the SNPs are very crucial type of variations which are very common and engage in a vital role in the human genetics pheno-type variations. These SNPs are the genetic basis of complex diseases in human. In human, the role of certain gene like B Cell Lymphoma 2 (BCL2) has got special attention because it?s over expression is resulting in certain type of lymphocytes cancer, because this gene encodes the outer mitochondrial membrane and blocks the apoptotic cell death in lymphocytes, resulting the formation of follicular lymphoma a type of cancer. In this research the role of Functional SNPs is studied by applying different computational methods to narrow down the mis-sense impact of deleterious SNPs. In This study 137 nsSNPs in BCL2 gene were investigated through computational techniques like SIFT, Polyphen2, I-Mutant 2.0 along with ConSurf web server and different nsSNPS were having common results studied for potential impact on the functioning and structural variations of BCL2 protein. Furthermore, Micro Dynamics Simulation analysis of the structure lead to the confirmation of effect of nsSNPs on the consistency and secondary property of BCL2 Protein. The tools like UTRScan (UTRdb), MirSNP, PolymiRTS and miRNASNP predicted to show the pattern changes in UTR-SNP. The recording of missense SNPs is very much beneficial for reduction of the mutations in BCL2 gene in genetic association studies to understand the function and structure related aspects of BCL2 Protein.
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منشی عطاء اﷲ

منشی عطاء اﷲ 
افسوس ۲؍ فروری کو دارالمصنفین کے مخلص اور قدیم کارکن منشی عطاء اﷲ صاحب نے داعی اجل کو لبیک کہا، ان کا اصلی وطن کیرانہ ضلع مظفرنگر تھا، ۱۹۱۶؁ء میں دارالمصنفین میں پریس قائم ہوا تو ان کے بڑے بھائی منشی عبدالحفیظ صاحب مرحوم نے اس کے انصرام کی ذمہ داری قبول کی، ان ہی کے ساتھ یہ بھی اعظم گڑھ آکر شعبۂ طباعت سے وابستہ ہوئے، جب کبرسنی کی وجہ سے ملازمت ترک کرنی چاہی تو سید صباح الدین صاحب مرحوم کا شدید اصرار اس میں مانع ہوا، پھر اپنے سعادت مند فرزند ڈاکٹر محمد نعیم ندوی کے اصرار سے گھر پر آرام کرنا منظور تو کرلیا لیکن ان کا دل دارالمصنفین ہی میں لگا رہتا تھا، اس لیے جب تک قوت رہی برابر دوسرے تیسرے روز یہاں آتے رہے، وہ طبعاً نیک، صلح پسند اور دیندار تھے، اپنی دینداری کی وجہ سے اپنے اکلوتے بیٹے ڈاکٹر محمد نعیم ندوی کو حفظ کرانے کے بعد ندوہ میں داخل کیا، جو فراغت کے بعد دارالمصنفین کے رفیق رہے اور اب ابوظہبی کے محکمۂ شرعیہ سے وابستہ ہیں، منشی عطاء اﷲ صاحب اپنی نیکی، بھلمنساہت اور ملنساری کی وجہ سے شہر میں مقبول تھے، اﷲ ان کی مغفرت فرمائے اور متعلقین کو صبر جمیل عطا فرمائے۔
(ضیاء الدین اصلاحی، فروری ۱۹۹۲ء)

 

مسئلہ ختم نبوت پر دلائل کا منہج و اسلوب : تجزیاتی مطالعہ

The belief of the finality of Prophet Hood (Khatm e Nabuwwat) is the basic belief of the Muslim Ummah, and this belief has been adopted since the first day. From the time of the Prophet ﷺ until now, the belief in the end of Prophet Hood has been protected on practical grounds and intellectually. Muslim scholars and philosophers, while strengthening it on the basis of rational and simulated arguments, did not leave a single aspect from which it could be undermined. They presented simulated arguments for the misguided sects in Muslim Ummah and for the non-Muslims, presented rational arguments in such a way that there was no reason left to disbelieve this belief. In this research article, there is a description of the simulated and rational arguments that are usually presented, such as the Quranic verses and their interpretation, the Hadiths, and the sayings of the scholars. And it has also been narrated what their style of reasoning is, in the same way, the legislation that has been passed in this regard in Pakistan has been presented in detail. Hopefully, this will make it clear what are the services of scholars and thinkers on this issue and what is their style.

Exploring Teachers Perceptions and Practices Regarding Formative Assessment in a Private School System in the Chitral District of Pakistan

This study aimed to explore the perceptions and practices of teachers regarding formative assessment (FA) in a private school system in the Chitral, district of the Khyber Pakhtunkhwa province of Pakistan. Altogether 123 teachers participated in the study from 25 selected primary, middle and high schools. Stratified random sampling was employed for the selection of the schools and simple random sampling for the teachers from each school. Questionnaire and a classroom observation checklist were used as data collection tools. The research questionnaire was aimed to explore teachers’ perceptions associated with their understanding, practices, their opinions about the advantages of formative assessment both for the students and teachers, limitations of formative assessment and the advantages of formative assessment records. The observation checklist was administered to observe the integration of formative assessment techniques in classroom operations such as planning for the lessons, its presentation, teachers’ communication skills, evaluation, classroom activities and classroom displays. The questionnaire and classroom observation checklist were administered through direct administration by visiting the schools. The data was analyzed using SPSS 22 software to calculate the percentage of responses, mean and standard deviation. The data was tabulated after cleaning it in MS Excel. Parametric and non-parametric tests were employed for comparative analysis of responses of independent variables such as teachers’ gender, academic and professional qualification, teaching experience and participation in continuing professional development programs. The major findings of the study show that teachers have positive perceptions about formative assessment and stated that they have sound understanding of formative assessment and are competent enough to use these techniques during teaching and learning processes. It was evident from classroom observations that teachers were using formative assessment techniques efficiently; nevertheless the teachers depended on few techniques; most of the time they would use some techniques in all situations. In comparative analysis the findings of the study varied to a less extent across independent variables as a slight difference was found among the groups.