The most common types for heredity changes are called as single nucleotide polymorphisms (SNPs).This has an important impact in the heredity different organisms to produce physical appearance changes and are claimed to be heredity changes lead for various mankind problems. Heredity changes can Hepatocyte nuclear factor behaves as switch which turns on and off other genes in the body. Thus by means of heredity can cause of mankind problems. About 70% of diabetes is caused by HNF1A gene. Our study will evaluate the deleterious effect of 40 nsSNP in the HNF1A gene using computational methods. We will use SIFT, PolyPhen-2, 1-Mutant Suite, and Conserve server for analyzing effect of the selected SNPs on arrangement, working and efficacy of the HNF1A gene. Apart from this, we will use SIFT, PolyPhen-2 and 1-Mutant Suite for analyzing effect of the selected SNPs on arrangement, working and efficacy of the HNF1Agene. Apart from this, impact of the selected SNPs for transcription and translation by HNF1A gene will also be analyzed through molecular dynamics simulation and structure analysis.