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Home > Computational Analysis of Functional Single Nucleotide Polymorphism Associated With Hnf1a Gene

Computational Analysis of Functional Single Nucleotide Polymorphism Associated With Hnf1a Gene

Thesis Info

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Author

Mian Zulqurnain

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=228

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721000464

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The most common types for heredity changes are called as single nucleotide polymorphisms (SNPs).This has an important impact in the heredity different organisms to produce physical appearance changes and are claimed to be heredity changes lead for various mankind problems. Heredity changes can Hepatocyte nuclear factor behaves as switch which turns on and off other genes in the body. Thus by means of heredity can cause of mankind problems. About 70% of diabetes is caused by HNF1A gene. Our study will evaluate the deleterious effect of 40 nsSNP in the HNF1A gene using computational methods. We will use SIFT, PolyPhen-2, 1-Mutant Suite, and Conserve server for analyzing effect of the selected SNPs on arrangement, working and efficacy of the HNF1A gene. Apart from this, we will use SIFT, PolyPhen-2 and 1-Mutant Suite for analyzing effect of the selected SNPs on arrangement, working and efficacy of the HNF1Agene. Apart from this, impact of the selected SNPs for transcription and translation by HNF1A gene will also be analyzed through molecular dynamics simulation and structure analysis.
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اسم ِ استفہامیہ : کیف؟ (کیسے)

اسم ِ استفہامیہ :کیف؟ (کیسے)

ارشارِ ربانی ہے:

"كَيْفَ نُكَلِّمُ مَنْ كَانَ فِي الْمَهْدِ صَبِيًّا"۔[[1]]

"لو بھلا ہم گود کے بچے سے باتیں کیسے کریں؟۔"

سیدہ مریم نے فرشتہ کی ہدایت کے مطابق ان کی کڑوی کسیلی باتوں میں سے کسی کا جواب نہ دیا بلکہ اس نومولود بچے کی طرف اشارہ کردیا کہ یہ خود جواب دے گا۔ اس بات پر لوگ اور زیادہ برہم ہوئے اور کہنے لگے ایک تو خود مجرم ہو دوسرے ہمارا مذاق اڑاتی ہو ۔ یہ بچہ جو ابھی پیدا ہوا ہے بھلا ان باتوں کا کیا جواب دے سکتا ہے؟



[[1]]         القرآن ، ۱۹:۲۹

عالمی مذاہب میں عورت کا مقام: تقابلی مطالعہ

Islam which is considered the religion and code of life of humanity is also the fore runner of the women rights. It not only restored their last glory as the sacred mother, daughter, wife and sister but also give them equal share in social life and give prime importance to them in decision making. As Islam is the youngest of all human religions therefore all the short comings regarding women rights is fulfilled and given in Islam. This comparative study will analyses the women status in all religions and will compare it with the same in Islam.

Phyto- Availability of Cadmium to Rice and Wheat in Contaminated Soils Treated With Organic Amendments

Cadmium (Cd) pollution is an emerging concern in many industrialial areas, mainly where raw effluents released from industry and cities. In many developing countries like Pakistan these effluents are used to raise grain crops and vegetables despite someof theses effluents having higher levels of metals such as Cd. In this thesis we investigate Cd availability and accumulation in wheat and rice following a series of four experiments. The first experiment was a hydroponic study executed to explore the effect of Cd on growth and micronutrient accumulation in wheat and rice. Cadmium was applied in three levels i.e. 5, 10 and 15 µM and nutrient solution was Hoagland. Results indicated that growth of both wheat and rice was significantly affected at higher Cd (15 µM) level. Increasing Cd levels significantly reduced gas exchange characteristics including photosynthetic rate and transpiration rate of both crops. Root and shoot Cd contents were highest, whereas micronutrient (Cu, Fe, Mn and Zn) concentration was minimum in 15 µM Cd treated plants of both crops. On the basis of solution culture experiment it was concluded that, Cd has significant effect on growth, physiological and micronutrient contents of wheat and rice especially at 15 µM Cd application. The second experiment was a pot study to evaluate the effectivesness of different organic amendments on reducing Cd availability to rice and wheat in an artificially Cd contaminated soil. Amendments used were cotton sticks biochar (CSB), wheat straw biochar (WSB), rice husk biochar (RHB), farm yard manure (FYM), poultry manure (PM) and press mud (PrMd) @ 2% (w/w). The results revealed that addition of organic amendments significantly improved growth and reduced shoot, root and grain Cd and AB-DTPA extractable soil Cd in wheat-rice cropping. The maximum Cd reduction in soil and plant was recorded with the application of PrMd and RHB in both crops whereas the highest Cd concentration was observed in unamended Cd applied soil. On the basis of this study, it was concluded that, addition of organic amendments especially PrMd and RHB improved growth and yield of both crops and reduced Cd uptake. The third trial was accomplished to study the role of selected organic materials on Cd phyto-availability and growth of wheat and rice in raw effluent irrigated farmer’s field at village Suraj Miani, suburbs of Multan city. Treatments were control; RHB; FYM; PM and PrMd @ 10 Mg ha-1 randomized in 9 m2 sized plots. The results indicated that RHB and PrMd showed higher gas-exchange characteristics and lower plant and AB-DTPA extracable soil Cd. The soil (0-15 cm) Cd was lowered by 33 and 32% in post-wheat whereas 48% in post-rice by the application of RHB and PrMd respectively over control. The economics of treatments showed that except RHB, addition of amendments gave higher benfit cost ratio over control. Among amendments FYM was found more economical followed by PM and PrMd. The fourth experiment was performed in pots to study the effect of organic acids on Cd availability to wheat and rice. Organic acids viz. oxalic acid (OA), citric acid (CA), EDTA and tartaric acid (TA) were applied @ 2 mM before sowing of wheat. Rice seeedlings were transplanted to similar (post-wheat) pots to observe the long term effectiveness of applied organic acids. The results showed that Cd in root, shoot and grain/paddy of wheat and rice was increased by addition of organic acids and found highest for EDTA as compared to rest of treatments. The post-harvest AB-DTPA extractable soil Cd was also higher with EDTA treatment. Over all, Cd stress reduced growth and yield of both wheat and rice in natural and Cd spiked growth medium. This shows that Cd stress is inversely related with growth, yield and micronutrient uptake, whereas Cd uptake and concentration is directly related with Cd concentration in growth medium. Application of organic amendments especially PrMd and RHB improve growth, yield and photosynthetic attributes of both crops in artificially (spiked) Cd contaminated soil and raw sewage irrigated field as a result of less Cd uptake and translocation. Addition of organic acids especially EDTA formed soluble chelates with Cd thus increased Cd phytoavailability and uptake by wheat and rice.