With an increase in the demand for skin regeneration products, there is a noticeable increase in developing such materials that have novel properties, ability to heal wounds and to regenerate skin. It has also been observed that antioxidants play an important role in anti-inflammatory reactions, cellular proliferation and remodeling phase of wound healing. Keeping under consideration all of these factors, a novel a-tocopherol acetate/vitamin E (VE) loaded bi-layered electrospun membrane, based on lower polycaprolactone (PCL) layer and upper polylactic acid (PLA) layer, was fabricated through electrospinning. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), in vitro degradation studies, swelling studies and VE release studies were performed to check out the structural, physical and in vitro behavior of membranes. Biological properties of membranes were evaluated through cell proliferation assay, cell adhesion studies, live/dead cell assay and wound healing assay. SEM images showed that the average diameter of nanofibers ranged from 1um-6um while addition of VE changed the diameter and morphology of fibers. Bi-layered membranes showed significant swelling behavior through water uptake and membranes loaded with 30% VE showed 8.7% and 6.8% degradation in lysozyme and H2O2 respectively. 20% and 30% VE loaded membranes followed Kosmeyer-Peppas and first order drug release kinetics followed by non-fickian drug release kinetics. Membranes showed non-toxic behavior and supported cell proliferation via alamar blue assay, cell adhesion via SEM, cell viability via live/dead assay and wound healing by scratch assay. Results showed that large surface area of nanofibers, porous structure and biocompatible nature will provide potential application of these bi-layered electrospun membranes in wound healing.
قاضی سلطان محمود مرحوم کو ہم سے بچھڑے پانچ سال ہو گئے ۔میرے سیاسی استادوںمیں دو لو گ شامل ہیں ۔قاضی سلطان محمود اور سابق سینئر استاد سردار سلیم صاحب ۔جن سے نہ صرف بہت کچھ سیکھا بلکہ 1978ء میں شروع ہو نے والا مزاحمتی سیاست کا سفر ان کے ساتھ راولپنڈی اس لحاظ سے خوش قسمت شہر تھا جسے پاکستان پیپلز پارٹی کی سیاست میں نہ صرف نمایاں مقام حاصل رہا ہے بلکہ سردار سلیم صاحب اور قاضی سلطان محمود مرحوم جیسی بہادر قیادت ملی دونوں بائیں بازو کی ترقی پسند سیاست پر یقین رکھتے تھے ۔ان کی سوچ فکر نے میرے جیسے سینکڑوں کا رکنوں کی سیاسی تربیت کی جنہوں نے آگے چل کر جنرل ضیاء الحق کے خلاف جمہوریت کی بحالی کی جنگ میں بھرپور کردار ادا کیا ۔
چھوٹے قد کا بڑا آدمی جنرل ضیاء الحق کے مارشل لاء کے خلاف مزاحمتی تحریک کا ایک اہم کردار سلطان محمود قاضی۔ 1979ء بلدیاتی انتخابات کا بگل بج چکا تھا راولپنڈی میں ٹکٹوںکی تقسیم ہو چکی تھی ہمارے حلقہ میں سنا تھا کہ ٹکٹ شیخ جلیل اختر مرحوم کو ملا ہے جو پی پی پی وارڈ کمیٹی ڈھیری حسن آباد کے چئیر مین تھے ۔ایک صبح دروازے پر دستک ہوئی میں نے دروازہ کھولا تو سامنے ملک الطاف حسین مرحوم کھڑے تھے ۔میرا ان سے کوئی تعارف نہ تھا لیکن بلدیاتی انتخابات کے بعد تو ملک صاحب میرے قریب ترین دوستوں میں شامل ہوئے ۔شاہی قلعہ تک اکٹھے گئے ۔ملک الطاف حسین مرحوم نے بتا یا کہ انہیں پاکستا ن پیپلز پارٹی نے وارڈ نمبر 5سے ٹکٹ دیا ہے ۔میں نے انہیں کہا کہ میری تو ابھی ووٹ بھی نہیں بنی میں میٹر ک کا طالب علم ہوں تو انہوں نے ہنستے ہو ئے...
This study examines the association of living areas (slum and non-slum) with the selected public health-related variables in the presence of socioeconomic variables among married women and also having a child. A total of 150 women aged 18 to 49 are selected of which 50 women from slums and 100 women are from non-slum areas of Multan by applying the cluster and random sampling techniques. The cross-tabulation method is used to find the results. The dependent variable is Body Mass Index (BMI) and it is analyzed with the socio-economic variables such as mass media index, household characteristics and education. The findings exhibit that the BMI of the women living in the slum areas is low due to a low level of education, lack of mass media access, bad household structures, and poor or ignorant area. The women of the slum area have fewer mass media access, poor status of household characteristics and less education as compared to the women living in the non-slum areas. BMI is significantly affected by area, women's education and household characteristics except for physical work, job status, mass media access and husband education. The findings of this study suggest that to provide health facilities or to reduce the gap in public health, education, mass media access and households characteristics might be considered while making any decision related to the slum and non-slum areas.
The present study was undertaken to determine the comparative prevalence of streak canal infections in buffalo and cow and to determine the comparative dimensional features of streak canal these two dairy species. In addition, comparative efficacy of partial or full insertion of teat cannula of a dry period antibiotic formulation in buffalo and cow was determined. Duplicate streak canal swab and quarter foremilk samples taken from 20 lactating buffaloes and 20 lactating cows were cultured and isolates identified. Length and width of streak canal of buffalo and cow (n = 20 each) was determined by Vernier caliper after incising the teats collected from abattoir. An in vivo comparative radiographic analysis of streak canal of buffaloes and cows (n = 20 each) was performed after infusion of 0.25ml of 5% barium sulphate into each teat followed by radiographic imaging and measurement of streak canal length and width from the image. In another phase of the study, efficacy of partial insertion vs. full insertion of teat cannula of a commercial cephapirin benzathine dry period antibiotic formulation (Cefa-Dri®) in the prevention of new intramammary infections and treatment of existing infections was evaluated on 20 buffaloes and 20 cows. Results: Seventy three (91.25%) and 59 (73.75%) of streak canal swab samples of cow and buffalo respectively were found infected. Staphylococcus aureus was the most frequent isolate both in cow and buffalo accounting respectively for 34 and 18 of total isolates (n = 106 for cow; n = 78 for buffalo) recovered from streak canal. Forty four (55%) and 18 (22.5%) of quarter foremilk samples of cow and buffalo respectively were infected. Staphylococcus aureus was the frequent isolate recovered from milk samples of both cows and buffaloes accounting respectively for 25.49% and 18.18% of total milk isolates. The mean values of streak canal length determined by Vernier caliper in cow and buffalo respectively were 9.78 ± 1.20 and 9.96 ± 1.44 mm whereas the mean width was 0.9 ± 0.12 and 0.98 ± 0.23 mm; both dimensions being non significantly different in the two dairy species. The mean length (9.34 ± 1.01mm) of streak canal of cow determined from radiographic image differed non- significantly (p = 0.105) from the mean length (9.97±1.36mm) of streak canal of buffalo. Similarly, mean width of streak canal of cow (0.9±0.09mm) differed non-significantly (p = 0.192) from the mean width of streak canal of buffalo (0.94±0.10mm). Thickness of epithelium of streak canal of buffalo (357.19 ± 4.8μ) was highly significantly (p = 0.001) more than thickness of epithelium of streak canal of cows (327.25±5.91μ). Similarly, thickness of keratin layer of streak canal of buffalo (154.7±6.34μ) was highly significantly (p = 0.001) greater than the thickness of keratin layer of streak canal of cow (101.3±4.11μ). As also was the case for muscle sphincter thickness around streak canal. Fürstenberg’s rosette folds were highly significantly (p = 0.001) thicker in buffalo (13.4±0.12) than in cow (12.75±0.2). Mitotic index of streak canal of cow and buffalo also differed significantly (p = 0.038); being greater in cow than in buffalo. Seventeen new intramammary quarters infections were detected in 40 quarters of cows treated by full insertion of dry intramammary treatment syringe (Cefa-Dri®, Forte Dodge, USA) cannula. The corresponding figure for 40 cow quarters treated by partial insertion of the same antibiotic dry period formulation was 6. Nine new intramammary infections developed over dry period in 40 quarters of buffaloes treated by full insertion of treatment syringe (Cefa-Dri®, Forte Dodge, USA) cannula. The corresponding figure for 40 buffalo quarters treated by partial insertion of the same antibiotic dry period formulation was 3. A total of 20 quarters of 10 cows were found infected by various mastitis pathogens at drying off in full insertion treatment group. Thirteen of these quarters were found cured after calving as no organism could be isolated from samples collected at calving and those collected biweekly for 3 months after calving. In the partial treatment group, of the 22 quarters, 18 were cured. Thus the cure rate (81.8%) in the partial insertion treatment group was significantly higher than in the full insertion treatment group in cows. Ten of the 14 (71.4%) infected quarters of buffaloes were cured when Cefa-Dri® intramammary tube was used by full insertion method. The corresponding cure rate by partial insertion of the same intramammary preparation was 84.6% (11 of 13 infected quarters being microbiological negative at calving as well as 3 months following calving of buffaloes). All in all, the results of comparative histomorphological examination of streak canal of cow and buffalo as well as the results of comparative efficacy trial of partial vs. full insertion of cannula of dry period antibiotic treatment syringe suggest that both the dairy species should be treated at drying off by using partial insertion of dry period intramammary antibiotic formulation.