Search or add a thesis

Advanced Search (Beta)
Home > Evaluation of Biological Properties of A-Tocopherol Acetate Loaded Bi-Layered Membranes in Wound Healing Applications and Skin Regeneration

Evaluation of Biological Properties of A-Tocopherol Acetate Loaded Bi-Layered Membranes in Wound Healing Applications and Skin Regeneration

Thesis Info

Access Option

External Link

Author

Saba Zahid

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=234

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721002327

Asian Research Index Whatsapp Chanel
Asian Research Index Whatsapp Chanel

Join our Whatsapp Channel to get regular updates.

Similar


With an increase in the demand for skin regeneration products, there is a noticeable increase in developing such materials that have novel properties, ability to heal wounds and to regenerate skin. It has also been observed that antioxidants play an important role in anti-inflammatory reactions, cellular proliferation and remodeling phase of wound healing. Keeping under consideration all of these factors, a novel a-tocopherol acetate/vitamin E (VE) loaded bi-layered electrospun membrane, based on lower polycaprolactone (PCL) layer and upper polylactic acid (PLA) layer, was fabricated through electrospinning. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), in vitro degradation studies, swelling studies and VE release studies were performed to check out the structural, physical and in vitro behavior of membranes. Biological properties of membranes were evaluated through cell proliferation assay, cell adhesion studies, live/dead cell assay and wound healing assay. SEM images showed that the average diameter of nanofibers ranged from 1um-6um while addition of VE changed the diameter and morphology of fibers. Bi-layered membranes showed significant swelling behavior through water uptake and membranes loaded with 30% VE showed 8.7% and 6.8% degradation in lysozyme and H2O2 respectively. 20% and 30% VE loaded membranes followed Kosmeyer-Peppas and first order drug release kinetics followed by non-fickian drug release kinetics. Membranes showed non-toxic behavior and supported cell proliferation via alamar blue assay, cell adhesion via SEM, cell viability via live/dead assay and wound healing by scratch assay. Results showed that large surface area of nanofibers, porous structure and biocompatible nature will provide potential application of these bi-layered electrospun membranes in wound healing.
Loading...
Loading...

Similar Books

Loading...

Similar Chapters

Loading...

Similar News

Loading...

Similar Articles

Loading...

Similar Article Headings

Loading...

19. Mary/Sayedah Maryam

19. Mary/Sayedah Maryam

I/We begin by the Blessed Name of Allah

The Immensely Merciful to all, The Infinitely Compassionate to everyone.

19:01
a. Kaf. Ha. Ya. `Ayn. Sad.
19:02
a. This is a narrative of the mercy of your Rabb - The Lord towards HIS servant, Zachariah.
19:03
a. When he called out to his Rabb - The Lord in seclusion by the middle of the night,
19:04
praying:
O ‘My Rabb - The Lord!
Indeed my bones have become weak within me, and my head is aflame with gray hair because of old age.
However - O My Rabb - The Lord - I have never been disappointed when calling out to YOU’ before –
- so do not disappoint me now.
19:05
‘Now, I worry who will be the heir after me to fulfill their duties to the Temple of Solomon
for my wife has always been infertile.
So grant me out of YOUR Mercy an heir’ -
19:06
- the one who will be my heir in my duty, and heir to the legacy of spiritual purity, divine knowledge, and Allah-consciousness of the House of Jacob;
and make him - O My Rabb - The Lord - the one with whom YOU would be pleased.’
19:07
His prayer was answered:
‘O Zachariah!
Truly WE give you the happy news of the birth of a son.
His name will be Yahya/John the Baptist.
The uniqueness of the name is that WE have never assigned this name to anyone before.’
19:08
Zachariah exclaimed with joy and surprise:
O ‘My Rabb - The Lord!

But how can I have a son while my wife has always been infertile, and I have already become frail because...

علماء اہلسنت (بریلوی) کی تفاسیر کا اسلوب بیان: ایک جائزہ

The Holy Quran is the first and very important source of Islamic Law. This book was revealed in Arabic language. When Islam spread over the Subcontinent with the passage of time then It was needed to translate this book in the regional languages for the better understanding of Allah's message. The people of this era could not understand the real teaching of Quran without its translation. The Ulema e Ahlesunat (Barelvi) school of thought took participation in the field of Tafseer to convey this holy message to the people of Subcontinent. The intellectual efforts of the scholars of the Ahlesunat (Barelvi) thoughts can be found in every field of Islamic teaching. These Ulema extended their contributions in Islamic teaching through illustration of the Quran. They wrote translations of the Quran in different ages and tried to solve the problems which were raised in this era about Islamic teaching. In this research article the authors analyzed the style of selected Mofasereen of the Ahlesunat( Barelvi's )school of thought

Plasma Processing of Materials Using Plasma Focus

The work presented in this thesis addresses the parametric study of ion beams emitted from Mather type plasma focus devices and their flourishing utilization in materials processing. Experiments have been performed by using two different plasma focus devices; a conventional 2.3 kJ plasma focus device developed under the joint venture of the United Nations University (UNU) and the Abdus Salam International Centre for Theoretical Physics (ICTP) designated as the UNU/ICTP device operational at the GC University Lahore and a modified version called the Nanyang X-ray source-2 designated as the NX2 device (a repetitive plasma focus) operational at the National Institute of Education (NIE), Nanyang Technological University (NTU), Singapore. The measurements of ion parameters such as energy, energy distribution, number density and current density are carried out in the ambient gas pressure by employing a BPX65 photodiode and a Faraday cup (FC) using time of flight technique. A major motivation is to establish the optimum processing conditions for ion nitriding, surface modification, phase changes and carburizing of materials of industrial interest like Ti, AlFe 1.8 Zn 0.8 alloy and SS-321 in plasma environment. The processed samples are characterized for structural and morphological changes, compositional profile and surface hardness by employing X-ray diffraction (XRD) at GC University Lahore, scanning electron microscopy (SEM) at University of Peshawar, field emission SEM (FESEM) and energy dispersive X-ray spectroscopy (EDX) at the NIE NTU Singapore, X-ray photoelectron spectroscopy (XPS) at the National University of Singapore (NUS) Singapore, Raman spectroscopy and Vickers microhardness test at Quaid-i-Azam University Islamabad, Pakistan. The SRIM code and microindentation measurements are used to estimate the depth profile of the modified layers. Nanocrystalline spatially uniform TiN thin films with petal like features are developed on Ti substrates exposed to 30 focus shots at various axial positions. The surface roughness and the relative proportion of the TiN films are strongly influenced by the ion beam energy flux. The film acquires eminent appearance with maximum relative proportion of nitrogen at 7 cm axial position. The probable energy of the ions reaching this position is 64 keV with the maximum ion number density of 5.9 ́10 13 cm -3 . The corresponding energy flux and current density are 2.69 ́10 13 keV cm -3 nsec -1 and 1142 A cm -2 viiirespectively. The grain size of the film is estimated to be about 90 nm while the compound layer thickness is about 0.66 μm. The surface microhardness is also maximum at this axial position with typical value of 7650±10 MPa. The SEM images of a typical microcracked TiN thin film and the SRIM code estimations of ion penetration help in understanding the growth mechanism of the film in terms of ion dose. The granular nanostructures appearing on the substrate surface are grown from nucleates of a few nm size developed by the energetic ions induced collision cascades. The predeposited nitride layer or nitrogen ions interstitially implanted into the substrate surface are also redistributed by the successive pulses of the ion beams leading to layer densification along with possible resputtering. Moreover, the temperature evolution during the DPF ions irradiation also enhances the reactivity of the nitrogen already introduced during the preceding pulses. The residual tensile stresses on the sample surface are transformed to the compressive stresses after DPF ion irradiation. Nitrogen ions induced surface changes in AlFe 1.8 Zn 0.8 alloy are investigated as functions of axial and angular positions for 30 shots. The expanded fcc phase of Al is evolved owing to the incorporation of nitrogen along with Fe and Zn into the Al lattice. A comparatively smooth and crack free nitride layer is formed on the sample treated at 7 cm axial and 10 0 angular position with 4- to 5-fold increase in Vickers hardness. TiN 0.9 and (Fe,Cr) 2 N are deposited on SS-321 along with formation of non-stoichiometric (Fe,Cr) x N phase by exposing the samples to multiple focus shots in nitrogen plasma at different axial and radial positions. The transformation from (Fe,Cr) x N to (Fe,Cr) 2 N is attributed to an increased nitrogen ion dose. The point-like structures of flakes reveal the nucleation of crystal growth with the increased ion doses. The nitride layer is golden in colour and is spatially uniform with improved surface hardness. Multiphase nanocrystalline titanium oxycarbide TiC x O y thin films composed of TiC 2 , TiO 0.325 , Ti 2 O 3 and carbon phases are deposited on titanium substrate in CH 4 discharges by the UNU/ICTP and the NX2 devices. The nanocomposite films are non-porous and microcrack-free with grain-like surface morphology having spatially uniform carbon distribution. XRD, Raman and XPS results reveal the favorable evolution of multiphase coatings having a stoichiometric TiC 2 phase and graphitic carbon adsorbates along with ixthe residual oxide (TiO 0.325 , Ti 2 O 3 ) phases with the lower energy flux and lower repetition rate in the UNU/ICTP treatment. Whereas, the deposition of carbon and a non- stoichiometric TiO 0.325 phase is favored due to the improved oxide removal and enhanced disorder in the substrate surface during the NX2 treatment. In addition, TiC 2 phase is also suppressed, possibly due to the enhanced substrate temperature caused by the higher energy flux of the ion beams and the higher repetition rate. The granular profile of the films attains a definite coagulation pattern. The energy flux of the ion beam and the repetition rate are found to be critical parameters which influence the preferred evolution of a particular phase during the restructuring of various phases.