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Home > Association Analysis of Ldl-Cholesterol With Celsr2, Apoe and Apob Genes Through Arms-Pcr

Association Analysis of Ldl-Cholesterol With Celsr2, Apoe and Apob Genes Through Arms-Pcr

Thesis Info

Access Option

External Link

Author

Umair Zubair

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=235

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721002394

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Dyslipidemia is term to demonstrate the abnormal lipid metabolism that may lead to cardiovascular diseases.Several variants have been already reported to be associated with abnormal lipid metabolism. Four different variants CELSR2 (rs629301), APOE (rs429358 & rs7412) and APOB (rs1367117) were analyzed by Tetra Primer-Amplification Refractory Mutation System Polymerase Chain Reaction (T-ARMS PCR).This technique uses an approach that in addition to amplifying the control fragment (which surrounds the allele of interest), the mismatch, at the point of SNP on allele, on 3? terminus in additional inner primers makes these primers specific to one allele and refractory to other one. An additional mismatch near the 3? terminus of the inner primers strengthen the allele specificity. An amplicon is produced or not produced, in this condition, thus demonstrate the genotype of the subject. Though the optimization and development of T-ARMS PCR was difficult and time consuming but the method allows to genotype the specific variant and was validated by Sanger Sequencing method. As with other techniques, this technique has also its advantages and disadvantages.Variants having high GC content cannot be genotyped because of having high melting temperature and therefore formation of stable secondary structures thereby halting the amplification procedure even when organic additives were added. Therefore T-ARMS assay could not be developed for APOE (rs429358 & rs7412) variants. The second part of the study was to find the association analysis of the selected variants. T-ARMS PCR assay was developed for the CELSR2 (rs629301) and APOB (rs1367117) variants. Individuals having normal and abnormal lipid profiles (n=25 each) were examined for association analysis. Genotype distribution were found to be in Hardy-Weinberg Equilibrium and Chi square test indicates the significant association for both variants. Large scale genotyping approach can be employed for other along with these variants by using plate-based heat-block on PCR machine. As T-ARMS PCR Technique is robust and cost-effective, early risk detection can save the time window of treatment and would ultimately enhance the life expectancy of the patients suffering from dyslipidemia.
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قاری محمد یعقوب

مولانا محمد یوسف بنوری/قاری محمد یعقوب
سیمینار میں مولانا محمد یوسف صاحب بنوری کی وفات حسرت آیات کی اطلاع ملی اورواپسی میں جناب قاری محمد یعقوب صاحب(کراچی)کے حادثۂ انتقال کاعلم ہوا توسخت صدمہ اورملال ہوا۔ رحمھما اﷲ رحمۃ واسعۃً۔ اکتوبر اور نومبر میں بعض ضروری علمی کاموں میں، میں اس درجہ مصروف رہا کہ برہان کی طرف بالکل توجہ نہیں کرسکا۔یہ نظرات لکھنے کے لیے بھی بڑی مشکل سے وقت نکال سکاہوں۔ آئندہ انشاء اﷲ’’وفیات‘‘کے زیر عنوان مرحوم بزرگوں کا تذکرہ ہوگا۔ [نومبر۱۹۷۷ء]

METODE PARTISIPATIF, CERAMAH, DAN DISKUSI PADA AL QURAN SURAT AS-SHAFFAT AYAT 102

This research is motivated by a phenomenon of poor communication between parents and children. In the world of education, there was a lack of approaches and methods used by educators in providing subject matter so that what was delivered by the educator did not achieve the expected goals. The problem in this research is how the participatory methods, lectures and discussions contained in the As-Shaffat letter verse 102. The purpose is to describe the educational methods contained in the As-Shaffat letter verse 102 in the form of participatory methods, lecture methods and discussion methods. This research is a Library Research. The method of interpretation used in this study is the method of interpretation of maudhu 'or thematic methods which interpret verses of the Qur'an based on specific themes. The method used in the explanation of the verse is the method of tahlili or analysis that explains the verses of the Qur'an by examining its aspects and revealing its whole purpose. The results of study and analysis, as well as data processing from various sources, obtained the first result that the educational method contained in the As-Shaffat verse 102 is a participatory method used by educators, which involves all elements in the educational process especially students, with active participation from participants students will facilitate educators in delivering the material to be given. Both lecture methods, in conveying information, especially learning materials can be done with the lecture method. An educator prioritizes good attitudes so that the material can be accepted by students. The third method of discussion is to involve students in giving their opinions. With the discussion in learning, the lessons will become warmer and will train students to be more courageous in opinion.

Nutritional and Biochemical Evaluation of Vitamin K Enriched Dietary Sources

The present investigation was an effort to explore the health claims of vitamin K enriched dietary sources i.e. cooked spinach and fermented soybean/natto against the menace of vitamin K deficiency. Initially, spinach and soybean were characterized with special reference to vitamin K contents followed by product development and finally bioefficacy study for the management of blood coagulation and vitamin K dependent proteins. The nutritional analysis indicated that spinach has ample amount of moisture followed by protein and fiber whereas soybean contains higher amount of protein and fat contents. The high performance liquid chromatography (HPLC) quantification of vitamin K revealed that phylloquinone was higher in spinach as compared to soybean. Amongst antioxidant extracts, methanolic extracts of spinach and soybean showed higher total phenolic, 2,2-diphenyl-1 picrylhydrazyl (DPPH) scavenging and antioxidant activities. During product development phase, four dietary products i.e. cooked spinach (T 1 ), reconstituted spinach (T 2 ), natto A (T 3 ) and natto B (T 4 ) were formulated. Characterization of soybean based prepared products showed improvement in nutritional status due to fermentation as compared to raw material. In the formulated products, cooked spinach (T 1 ) and natto A (T 3 ) attained higher scores for sensory profile from each category. On the basis of nutritional characterization, vitamin K contents and antioxidant potential, two best products i.e. T 1 and T 3 one from each raw material were selected for efficacy study. Bioevaluation study was carried out involving New Zealand rabbits through two sequential trials for validity of the results. Accordingly, two types of studies were conducted on the basis of different groups of rabbits i.e. study I (normal rabbits) and study II (vitamin K deficient rabbits). Moreover, three different types of dietary sources namely cooked spinach a source of phylloquinone (D 2 ), natto for menaquinone-7 (D 3 ) and synthetic menadione (D 4 ) along with control (D 1 ) were provided to the respective groups. Feed & water intakes and body weights of rabbits varied significantly (p<0.05) with vitamin K dietary sources in study II while these traits behaved non-significantly in study I. The blood coagulation parameters including bleeding, clotting and prothrombin & partial thrombinplastin times and international normalized ratio (INR) were significantly (p<0.05) reduced due to vitamin K enriched dietary sources in vitamin K deficient rabbits (study II) except fibrinogen level that was improved. The serum phylloquinone levels were increased momentously (p<0.05) during study I & II (trial 1) by 23.78, 16.71 & 10.64 and 47.58, 36.42 & 27.71% in D 2 , D 3 & D 4 groups, respectively as compared to control. Similarly, serum menaquinone-7 level was improved in D 2 , D 3 and D 4 groups by 2.59, 17.77 & 1.11 in study I and 12.74, 24.50 & 10.78% in study II (trial 1). The vitamin K dependent proteins i.e. osteocalcin was increased whilst, undercarboxylated osteocalcin (ucOC) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) were decreased due to vitamin K enriched dietary sources. Likewise trend for these traits was noticed in trial 2 of both studies. The liver & kidney functioning tests and hematological values were within normal range. From the present exploration, it is concluded that vitamin K enriched dietary sources containing menaquinone-7 and phylloquinone are effective to improve the serum vitamin K status and ameliorate the coagulation and vitamin K dependent proteins related abnormalities.