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Home > Phylogenetic Study for the Investigation and Analysis of Uncultured Soil Microbial Diversity of Pakistani Hospital?S Dumping Sites Using Sequence-Based Metagenomic Approach

Phylogenetic Study for the Investigation and Analysis of Uncultured Soil Microbial Diversity of Pakistani Hospital?S Dumping Sites Using Sequence-Based Metagenomic Approach

Thesis Info

Access Option

External Link

Author

Maria

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=238

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721003483

Similar


Hospitals play a vital role in health and well being of humans. However the biomedical waste generated by hospitals pose a serious threat to public health and environment due to their high potential of disease transmission through spread of pathogenic bacterial strains from hospitals dumpsites by any mode of contact. Therefore methods for the analysis of microbial diversity in the environmental samples surrounding the hospitals? dumpsites are required for better understanding of Hospitals Acquired Infections (HAI) transmission. Metagenomics provides solution to this problem through availability of methods that allows the isolation of those microbial DNA from environment that cannot be cultured and hence their role has never been studied in transmission of HAIs. This research study is objected to the investigation of microbial diversity present in the soil surrounding the dumpsites of hospitals in order to construct a profile of bacterial communities present in natural environment of hospitals that may potentially be involved in transmission of HAIs. In this research study the microbial diversity of soil samples taken from 10 different hospital?s waste dumpsites was examined. Direct DNA extraction is performed by using Metagenomic approach with the objective of isolation of not only cultured but un-cultured bacterial communities as well. PCR was carried out for all samples for detection of 16S rRNA gene. Amplified DNA is directed to 16S rRNA gene sequencing. After sequencing the Phylogenetic analysis is performed through Phylogenetic tree rendering for the analysis of evolutionary relatedness of extracted bacterial sequences among themselves and with their possible closest species. The results of this research study have provided with the profile of bacterial species present in the soils surrounding the hospitals? dumping sites and also their evolutionary relatedness with other bacterial species. The results provided by this study have promising contribution in investigation of microbial communities present in hospitals environments and their potential involvement in transmission of HAIs suggests the need of strategies and protocols for proper disposal of biomedical wastes generated by hospitals.
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شیخ نذیر حسین

شیخ نذیر حسین
(ڈاکٹر محمود الحسن عارف)
’’محترم شیخ نذیر حسین صاحب بڑے عالم و محقق اور علم و فن کے قدرداں تھے، ’’معارف‘‘ ان کا محبوب رسالہ تھا، اگر ان کی کوئی چیز تیار ہوجاتی تو اس میں اشاعت کے لیے بھیجتے، معارف میں کوئی مسامحت اور فروگذاشت ہوجاتی تو فوراً توجہ دلاتے، ایسے صاحب نظر اور دیدہ ور لوگ اب عنقا ہوگئے ہیں، جب تک وہ اچھے تھے شاید ہی کوئی مہینہ ناغہ جاتا ہو کہ ان کا خط نہ آتا ہو، ادہر علالت کی وجہ سے جب ان کے خطوط آنے بند ہوئے تو میں نے شعبہ اردو، دائرہ معارف اسلامیہ لاہور کو متعدد خطوط لکھے مگر ایک خط کا بھی جواب نہیں آیا کہ دفعتہ ۲۲؍ اکتوبر کو یہ تحریر اور منسلک خط موصول ہوا تو دھک سے رہ گیا، قارئین معارف سے درخواست ہے کہ ان کے لیے دعائے مغفرت کریں!
ربنا اغفرلنا ولا خواننا الذین سبقونا بالایمان۔ ‘‘[الحشر:۱۰] (ض)
پانچ سوا پانچ فٹ منحنی سا جسم، ابرؤوں کے بال موٹے اور بے ترتیب، کلین شیو، چہرہ اور کمر قدرے خمیدہ․․․․․․ یہ تمام باتیں ذہن میں رکھیں تو اس سے فوراً شیخ نذیر حسین صاحب کی شکل و صورت ذہن میں آتی ہے․․․․․․ وہ اپنی وضع قطع اور چہرے مہرے سے قطعاً کوئی عالمِ دین یا اسکالر نظر نہیں آتے تھے، بلکہ ایک عام سے فرد دکھائی دیتے تھے۔
۷۰؁ء کی دہائی کے آخری سالوں میں جب میں اردو دائرہ معارف اسلامیہ میں پہلے پہل آیا․․․․․․ تو میں نے ڈاکٹر سید عبداﷲ، پروفیسر سید امجد الطاف، پروفیسر عبدالقیوم کے ساتھ مذکورہ بالا․․․․․․ چہرے مہرے کے ایک شخص․․․․․․ کو دیکھا تو مجھے ان کی وضع قطع کی بنا پر تعجب ہوا کہ یہ کون صاحب ہیں، پھر مجھ پر منکشف ہوا کہ یہ تو اردو دائرہ معارف اسلامیہ کے مدیر شیخ نذیر حسین...

سیرت النبی ﷺ میں احتسابی عمل کی مثالیں: وفاقی محتسب پاکستان کے فرائض کا اختصاصی مطالعہ

In any society, the administration has the status of a pillar, and its good or bad move directly impacts society. There are institutions to compensate public complaints on government initiatives in many countries of the world that keep check on government administrations. For this purpose "Federal Ombudsman" was established in Pakistan. The main responsibilities of this institution include the execution to enjoin the right and forbid the wrong (Amr bil Ma’rūf wal Nahi ‘An al Munkar), monitoring government employees in administrative matters, hearing the complaints of the public against organizations and preventing them from violating individual rights. In this article, it has been reviewed that how we can seek guidance from the Sīrah of the Prophet (P.B.U.H) is in this matter, to evaluate the present system of "Federal Ombudsman". In addition, the main responsibilities of the "Federal Ombudsman" were discussed in the article. In conclusion recommendations have been made for further improvement.

Study of Half Metallic Ferromagnetism in Thin Films of Heusler Alloys

The possibility of half metallic behavior has been investigated in Co2Mn1-xCrxSi and Co2MnxTi1-xAl thin films prepared by DC magnetron sputtering. Structural, magnetic and transport properties have been studied, particularly with a view to understanding the role of disorder on these properties. The growth conditions for deposition of these films on glass substrate without any additional buffer layer were optimized by using Co 2MnSi as the starting material. Crystallographic order in the films improved with increased substrate temperature and films grown at a temperature of 773K were found to exhibit the ordered L21 structure. Ferromagnetic order in the films also improved with increasing substrate temperature. A saturation magnetization (MS) value of 1061emu/cc, close to the MS value of bulk Co2MnSi, was obtained for the film deposited at 773K. Based on these optimized conditions, thin films of quaternary Co2Mn1-xCrxSi (0≤x≤ 1) Heusler alloy were prepared. High resolution transmission electron microscopy images of films showed granular morphologies, crystalline growth, and an ordered L21 structure in compositions with x≤0.6. For higher Cr concentrations (x>0.6), secondary phases started to appear and crystallinity was lost for x=1 composition. The Co2CrSi composition was found to possess disordered A2 structure and the desired L21 ordered phase was not obtained presumably due to the known instability of the desired phase. Magnetization measurements showed that for x≤0.2 magnetization values followed the Slater-Pauling rule, while for 0.2<x≤0.6 the MS values fell short of the theoretically predicted values. This decrease in saturation magnetization was explained in terms of Co-Cr antisite disorder present in these films. The values of Curie temperature were observed to decrease with increase in Cr concentration. For compositions with x>0.2 the enhancement of the Co-Cr antisite disorder also manifested itself in the decrease in the spin wave stiffness co-efficient and a sign change of the temperature coefficient of resistivity (at x>0.6). A resistivity minimum, observed at low temperatures (in metallic compositions) and the crossover from metallic to semiconducting type resistivity with increasing x were explained as originating mainly in the antisite disorder. These effects were explained in the light of Kaveh and Mott’s model of disorder leading to weak localization. For compositions with x≤0.6, the low temperature resistivity could be explained as arising from electron-electron interactions and due to two magnon scatterings. The latter is considered as an indicator for half metallic ferromagnetism. Both the onset temperatures as well as the temperature range for the half metallic features were observed to decrease with increasing x. In the other quaternary Heusler alloy Co2MnxTi1-xAl (0≤x≤ 1) thin film series all the compositions exhibited B2 type structure due to the effects of antisite disorder. The values of saturation magnetization increased with increasing Mn content in the films and were in general agreement with the values obtained using the Slater-Pauling rule. The general trend of magnetization in these thin films, as noted for others half metals, was describable as that of a Heisenberg ferromagnet at low temperature and an itinerant ferromagnet at higher temperatures. Electrical resistivity as a function of temperature showed a change from a metallic type behavior for x≤0.25 to a semiconducting type for x>0.25. At lower temperatures (T<25K) metallic compositions in this quaternary series also showed the presence of a resistivity minimum. The resistivity behavior above the low T upturn in metallic compositions followed a dependence that was explained in terms of electron-electron scattering and one magnon scattering. The expected half-metallic behavior was not clearly evident in this series, presumably being destroyed by the presence of Co antisite disorder within the B2-type structure, as has been predicted by first principle calculations. Finally, magnetoresistance measurements were performed on various compositions in Co2Mn1-xCrxSi series at temperatures below and above the resistivity minimum and the results were found consistent with the low field transport being strongly affected by spin polarized intergrain tunneling.