Main focus is to study the three districts of Punjab as well as the nearby areas where Kajli breed exists. In this study Kajli breed of sheep from Sargodha, Khushab and Mianwali districts were used. Blood samples of 30 animals up to 5 ml were collected in tubes containing EDTA as anticoagulant. These samples were stored at 4?C. DNA from these blood samples were collected with phenol chloroform extraction method. DNA quality and quantity were tested by 1% agarose gel. Purification of DNA using the liquid/solid-phase approach can be traced back to 1979, when Vogelstein and Gillespie used silica in a glass powder form in their protocol to purify DNA fragments previously separated by agarose gel electrophoresis. These techniques absorb DNA under particular pH and salt content conditions through any of the following principles: 1) Hydrogen binding in the presence of a Chaotropic agent to a hydrophilic matrix, 2) Ionic exchange using an anion exchanger under aqueous conditions 3) Affinity and size exclusion mechanisms. The genomic DNA was extracted from the blood sample and were visualized and confirmed on 1% agarose gel. DNA content was similar in all samples represent that all breeds had similar genome. Position of band in similar region showed that all the breads belong to similar origin. Populations of Mianwali, Khushab and Sargodha regions are clustered into one group. The cluster patterns were concordant with their geographic localities and were also in agreement with our previous studies using microsatellite markers. Amino acid content was also analyzed from the blood samples to determine either there is any difference among sheep belonging to different areas. Majority of amino acids of different breads are similar with each other which showed that their origin is similar from Urial. However, alanine content in sheep located from Sargodha was higher than those located with other areas. Further, valine and isoleucine content of breed from Mianwali was higher than those located in other areas. It is concluded from the analysis that all breeds of Kajli sheep are similar. They might have similar origin from Urial and spread all over the Pakistan. The minute difference in these breeds is due to different kind of breeding that is done in different areas of Pakistan. In the areas of Noorpoor thal and Quaidabad random mating is common while interbreeding is done in various ways in different regions of Mianwali which cause slightly difference in the phenotype of the Kajli sheep.