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Home > Pcr Based Genetic Diversity in Kajli Sheep Breed in Punjab, Pakistan Using Mitochondrial Dna Cytochrome-B

Pcr Based Genetic Diversity in Kajli Sheep Breed in Punjab, Pakistan Using Mitochondrial Dna Cytochrome-B

Thesis Info

Access Option

External Link

Author

Aamir Mumtaz

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=241

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721004394

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Main focus is to study the three districts of Punjab as well as the nearby areas where Kajli breed exists. In this study Kajli breed of sheep from Sargodha, Khushab and Mianwali districts were used. Blood samples of 30 animals up to 5 ml were collected in tubes containing EDTA as anticoagulant. These samples were stored at 4?C. DNA from these blood samples were collected with phenol chloroform extraction method. DNA quality and quantity were tested by 1% agarose gel. Purification of DNA using the liquid/solid-phase approach can be traced back to 1979, when Vogelstein and Gillespie used silica in a glass powder form in their protocol to purify DNA fragments previously separated by agarose gel electrophoresis. These techniques absorb DNA under particular pH and salt content conditions through any of the following principles: 1) Hydrogen binding in the presence of a Chaotropic agent to a hydrophilic matrix, 2) Ionic exchange using an anion exchanger under aqueous conditions 3) Affinity and size exclusion mechanisms. The genomic DNA was extracted from the blood sample and were visualized and confirmed on 1% agarose gel. DNA content was similar in all samples represent that all breeds had similar genome. Position of band in similar region showed that all the breads belong to similar origin. Populations of Mianwali, Khushab and Sargodha regions are clustered into one group. The cluster patterns were concordant with their geographic localities and were also in agreement with our previous studies using microsatellite markers. Amino acid content was also analyzed from the blood samples to determine either there is any difference among sheep belonging to different areas. Majority of amino acids of different breads are similar with each other which showed that their origin is similar from Urial. However, alanine content in sheep located from Sargodha was higher than those located with other areas. Further, valine and isoleucine content of breed from Mianwali was higher than those located in other areas. It is concluded from the analysis that all breeds of Kajli sheep are similar. They might have similar origin from Urial and spread all over the Pakistan. The minute difference in these breeds is due to different kind of breeding that is done in different areas of Pakistan. In the areas of Noorpoor thal and Quaidabad random mating is common while interbreeding is done in various ways in different regions of Mianwali which cause slightly difference in the phenotype of the Kajli sheep.
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مولانا مفتی عتیق احمد فرنگی محلی

مولانا شاہ عزالدین مجیبی/مولانا مفتی عتیق احمد فرنگی محلی
اس واقعہ سے چند روز پہلے یعنی مئی میں ہندوستان کے دواوربلند پایہ علماء کابھی انتقال ہوگیا،ایک مولانا شاہ عزالدین صاحب مجیبی اوردوسرے مولانا مفتی عتیق احمد فرنگی محلی۔ اوّل الذکرنے ندوۃ میں تعلیم پائی تھی، استعداد پختہ تھی، فقہ اور حدیث میں بڑااچھا درک رکھتے تھے۔ گزشتہ سال ہی انہیں صدر جمہوریہ کی طرف سے عربی اسکالر کی حیثیت سے ایوارڈ ملا تھا۔ قومی معاملات ومسائل میں حصہ لیتے رہتے تھے۔قیام خانقاہ مجیبہ پھلواری شریف میں رہتا اوروہیں درس و افتا کاکام کرتے رہتے۔ برہان کے بڑے قدردان تھے اوراسی وجہ سے ایڈیٹر برہان سے محبت کرتے تھے۔
ثانی الذکر فرنگی محل کے کاروان بہار کی آخری نشانی تھے۔بلند پایہ عالم اور بڑے فاضل بزرگ تھے۔ فرنگی محلی کے مفتی تھے اور اسی کے مدرسہ میں جواب برائے نام رہ گیا ہے، درس واہتمام کی خدمت بھی انجام دیتے تھے۔ گوشہ نشین اورقناعت پیشہ بزرگ تھے۔ [جون۱۹۷۷ء]

 

الأدب والاختلاف في أصالته

The word (Adab) , contrary to its present equitant in English language (literature) , has an interesting background and exciting history. This word went through many developments in its meaning and senses throughout the history of Arabic literature. Linguistics took keen interest in each and every change and development came into its meaning. They thoroughly studied this word, and tried to link between its early root meaning (giving a banquet or feast) , and its present meaning (literature) . Linguistics7 this attempt resulted in out break of differences among them regarding the word (Adab) . This article deals with this issue.

Processing-Efficient Distributed Adaptive Rls Filtering for Computationally-Constrained Platforms

Achieving fast convergence on an energy-limited and computationally-constrained platform still remains a dream in spite of magnificent advancements in Integrated Circuit (IC) technologies. For instance, in telephony, the echo cancellation re quires a high-definition adaptive-filtering algorithm that further needs a robust convergence performance while tracking the time varying uncertainties present in the communication link. Nevertheless, such high definition adaptive algorithm cannot be run on an energy-limited and computationally-constrained inexpensive platform. The research work in this thesis focuses to propose the low-complexity distributed adaptive filtering solution for energy-constrained platforms. The thesis is orga nized in three parts. Part-1 aims to develop a low-complexity MIMO channel estimation algorithm for MIMO communication system. Part-II and III pro vide the distributed and diffusion based adaptive signal processing solutions for computationally-constrained inexpensive platforms. The thesis begins with an overview of the adaptive algorithms with implementa tion constraints and then proceeds towards a comprehensive and detailed literature survey. The literature survey can be classified into two major areas, i.e. adaptive filter theory and adaptive algorithm implementation over low-cost platforms. Fur thermore, a channel model is presented with the consideration of two multipath components for MIMO communication environment. Taking it as a reference as channel model, a spatiotemporal low-complexity adaptive estimation algorithm is proposed by assuming time-variant block fading channel with fixed number of training symbols. The proposed algorithm exhibits better results than those shown by some notable least square algorithms in the literature. The effect of varying doppler rates on the convergence performance of the algorithm is thoroughly ob served to check the validation of the algorithm. Obtained simulated results show that the proposed algorithm entails low-complexity and provides independency on forgetting factor as compared to notable adaptive filtering algorithms. x In the second part of the thesis, a novel processing-efficient architecture of a group of inexpensive and computationally-constrained small platforms is proposed for a parallely-distributed adaptive signal processing (PDASP) operation. The pro posed architecture is capable of running computationally-expensive procedures like complex adaptive algorithms cooperatively. The proposed PDASP architecture operates properly even if perfect time alignment among the participating plat forms is not available. Complexity and processing time of the PDASP scheme are compared with those of the sequentially-operated algorithms. The comparative analysis shows that the PDASP scheme exhibits much lesser computational com plexity parallely than the sequentially-operated algorithms. Moreover, for high and low doppler rates, the proposed architecture provides a parallely-decreased processing time than the sequentially-operated MIMO algorithms. In part III, a novel distributed diffusion-based adaptive signal processing (DDASP) architecture for computationally-constrained small platforms is introduced. In the proposed DDASP architecture, the adaptive algorithm is diffused into the desired number of processing devices. The number of processing nodes that are used in DDASP architecture is dependent upon the number of MIMO channel streams as well as on the number multipath components. Therefore, having more nodes and diffusion mechanism, the proposed DDASP architecture exhibits lesser and linear computational complexity parallely on each processing node involved as compared to the proposed PDASP architecture.