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Home > Molecular Based Identification and Association of Mycobacterium Tuberculosis, Hbv and Hiv Virus in Patient Samples of Muzaffargarh District

Molecular Based Identification and Association of Mycobacterium Tuberculosis, Hbv and Hiv Virus in Patient Samples of Muzaffargarh District

Thesis Info

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Author

Khadija Tul Kubra

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2018

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=244

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721004807

Similar


Hepatitis is a major disease spreading more rapidly in all over Pakistan and its detection is increasing day by day. Knowledge of the distribution of Hepatitis B and HIV virus have important clinical implications since the efficacy of current and new therapies. The treatment of chronic Hepatitis B virus (HBV) infection has the potential to change significantly over the next few years as therapeutic regimens are rapidly evolving. However, the burden of chronic infection has not been quantified at the global level using the most recent data. Cohort and cross sectional study 534 blood samples randomly selected patients of pulmonary or extrapulmonary tuberculosis (T.B) was calculate utilizing Quantitative Polymerase chain reaction (qPCR). We examined Hepatitis B and HIV virus by ELISA and confirmation by qPCR. Risk factors estimated and study association among the triple or Co-infection of Hepatitis B virus, HIV virus, and mycobacterium tuberculosis in dissimilar period of life and groups of patients. Distribution of co-infection was diverse in regions. Decision between the 534 positive T.B patients analysis, 498 (93.2%) patients were negative and 36 (6.7%) were positive for HBV and 04 patients were positive for HIV (0.74%) by using the qPCR technique. The transference of HBV was estimated to have take place by and large between ages of 20-40, unmarried, poor and illiterate. Detection rate is high of HBV in males rather than females. There was developed a correlation between co-infection of HBV, HIV and tuberculosis patients due to low immunity and body mass index (BMI). We found that HBV can be effect on TB patients. Active tuberculosis is leading to the cause of death living with HBV and HIV virus. There is need for standard operating protocols (SOP?s) for better management of tuberculosis patients.
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مولانا محمد جلیل کیرانوی

مولانا محمد جلیل کیرانوی
افسوس ہے کہ گزشتہ ماہ اگست میں حضرت شیخ الہندؒ کے دومنتسبین،مولانا محمد جلیل کیرانوی استاذ اورمولانا محمد مبارک علی نائب مہتمم دارالعلوم دیوبند واصل بحق ہوکر اس جہانِ فانی کوالوداع کہہ گئے۔اناﷲ وانا الیہ راجعون۔ اوّل الذکر (المتولد۱۳۱۸ھ)نے اگرچہ دورۂ حدیث حضرت الاستاذ مولانا محمد انورشاہ کے عہد میں تمام کیا تھا لیکن درحقیقت پروردہ تھے حضرت شیخ الہند کے گھرانے کے ہی۔ نوبرس کی عمر تھی کہ اُن کے والد حضرت ؒ کے سپردکرگئے تھے۔یہ اس آستانۂ قدس کوایسے چمٹے کہ مرتے دم تک اسے نہ چھوڑا۔اس لیے حضرت شیخ الہند کے خادم خاص اور شریکِ جلوت وخلوت تھے اس بناء پر حضرت شیخ الہند کی مشہور’’ریشمی خطوط‘‘والی تحریک کے جزوکل سے خوب واقف اوراس کے محرمِ اسرار تھے۔اس سلسلہ میں انھوں نے بڑے بڑے مصائب اورشدائد برداشت کئے لیکن تحریک کابھید آشکار نہیں کیا۔حضرت ؒ کی وفات کے بعد ادہر ادہرمدرس رہے۔آخر میں دیوبند آگئے تھے اوردرس کی خدمات انجام دیتے تھے۔
[ستمبر۱۹۶۸ء]

 

قیام امن کے لئے حضرت عمر فاروق ؓکی خدمات

Peace has great importance both for the individual and the communal life. Wherever peace turned into unrest, the tendencies of social violence, mental sickness and insecurity start developing amongst the people. Peace and harmony were the hallmark of the reign of the 2nd caliph, Haḍrat ‘Umar. He gave the best governing mechanism to the people of Arab, when they were not fully aware of rules & regulation of government. Though the empire was wide spread, he exercised a great sort of command & control on it. He took the responsibility of providing his subjects their basic needs: Food, Shelter, Education, Peace and Justice. This was not only an ideal system of its time but became the role model for the modern welfare state. Peace and harmony is as important for a state as food & air are for life. Allāh has strongly emphasized in The Holy Qur’ān" on two things i. E., "Disharmony & hunger" which should be eliminated from a society. Haḍrat ‘Umer during his reign of 10 years presented Islām as a religion of peace & harmony, a religion, which respects humanity, peacefully resolves disagreements and curtails misuse of power. He himself possessed the qualities of peace & harmony to an utmost level, which were the traits of our Holy Prophet’s (ﷺ) personality. It is important to follow the Khilāphah of Haḍrat ‘Umar to bring peace & justice in the society.

Exploring the Genetic Diversity and Population Structure in Chickpea Cultivated Germplasm & Genetic Basis of Flower Color Polymorphism in Chickpea Cicer Arietinum L.

Assessment of genetic diversity and genetic framework/structure in chickpea crop has important effects on plants breeding programs and preservation of inherited resources. New types of markers have improved our ability to quickly and cost effectively uncover potentially useful variations in large chickpea germplasm collections in gene banks. Single nucleotide polymorphism (SNP) are recently developed markers, which are very effective in discovering inherited diversity. Little is known about the level of genetic diversity in these accessions to advance elite varieties. Chickpea production is predominant in arid & semiarid regions, such as in Pakistan, faces immense challenges of drought and heat stress. Addressing these challenges has made more difficult outstanding to lack of genetic and phenotypic characterization of available cultivated varieties and breeding materials. Genotyping-by-sequencing offers a rapid and cost-effective means to identify genome-wide nucleotide variation in crop germplasm.In this study, we have compared genetic variations and population structure of a previously uncharacterized collection of chickpea cultivated germplasm. Here, we used 31,995 SNP markers to estimate the genetic diversity and population structure in collection of 952 landraces and elite cultivars from the second centers of diversity and Fertile Crescent (Ethiopia, India, Pakistan & Turkey). For Pakistani accessions, we used 8,113 SNP markers to determine genetic variations and compare population structure within 77 landraces and 5 elite cultivars, currently grown in situ on farms throughout the chickpea growing regions of Pakistan. The compiled landraces span a striking aridity gradient into the Thal desert of the Punjab Province, Pakistan. Despite low levels of variations across the collection and limited genetic structure, we found some differentiation among accessions from arid, semi-arid, irrigated, and coastal areas. In a subset of 232 markers, we discovered evidence of differentiation along gradients of elevation and isothermality. Our results highlighted the utility of exploring large germplasm collections for nucleotide variation associated with environmental extremes. And further to use this data to nominate germplasm accessions with potential to improve crop drought tolerance and other environmental traits.To investigate the basis of genetic factors controlling flower color in chickpea, molecular and genetic characterization of colored flower and white flower chickpea accessions were performed. This unique white flowered color RS11 Chickpea accession lacking the anthocyanin in flower tissues. The genetic constitution of this accession is different to other white flower chickpea accessions because when it was crossed to another white flower color accession, they produced colored F1. None of white flower chickpea can synthesize mRNA corresponding to Leucoanthocyanidin dioxygenase (LDOX) gene, also called anthocyanidin synthase (ANS) on B and C locus. Molecular analysis of white chickpea revealed the presence of full deletion Intron, spanning both exon with in coding region of LDOX gene. Phenotyping and genotyping of F2 generations from cross between RS 11 (white flower) and 96029 (color flower) revealed segregation for flower color according to the Mendel’s pattern of segregation. Color and white flower phenotype demonstrated its complete linkage with the deletion in LDOX gene inherited as a recessive gene trait. Taken together the findings indicated that mutation in LDOX genes which is present on C locus here in RS 11 is responsible for white flower color in this chickpea accession.