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Isolation and Identification of Polyhydroxy Alkanoates Accumulating Bacteria from Sugar-Cane Industry Waste

Thesis Info

Access Option

External Link

Author

Farzana Yasmin

Institute

Virtual University of Pakistan

Institute Type

Public

City

Lahore

Province

Punjab

Country

Pakistan

Thesis Completing Year

2019

Thesis Completion Status

Completed

Subject

Software Engineering

Language

English

Link

http://vspace.vu.edu.pk/detail.aspx?id=299

Added

2021-02-17 19:49:13

Modified

2024-03-24 20:25:49

ARI ID

1676721017746

Similar


Polyhydroalkanoates (PHAs) are polyesters that are synthesized by different microorganism and stored as granules in microbes. They are used as a food under starvation. Studies have shown that physical properties of different PHAs resemble significantly to the petroleum based plastics and polyethylene. Additionally, microbial PHAs can be spun, mould and converted into filaments. On the other hand, PHAs are biodegradable material. Such promising features make PHAs as a potential candidate for next generation plastic as it will reduce the pollution caused by the petroleum based plastics. However, PHAs are costly as compared to the different petroleum based plastics. Therefore, researchers are trying to identify new microbes that may produce high amount of PHAs from waste carbon sources. They can accumulate high concentration of PHAs. During the crushing season sugar industries are producing tones of organic waste, specifically they are draining a considerable amount of sugar in canals. This can be a source of food for different types of bacteria, specifically for PHA accumulating bacteria. In this study, different PHAs accumulating bacteria were identified from the swage of sugar industry waste. Five soil and sewage samples were collected and stored at room temperature. For isolation and identification of PHAs accumulating bacteria, Samples were allowed to grow on LB agar medium plates supplemented with 1% Nile blue A. Different concentrations of Nile blue A were used for different incubation periods and colonies were screened. All these colonies were observed under ultraviolet light; PHAs accumulating bacteria were showed bright orange color inflorescence while non PHAs accumulating bacteria appeared white. The PHAs accumulating bacteria were selected and then amplified for 16S rRNA gene sequencing through PCR. In this study we have identified five PHAs accumulating bacteria from the waste of sugarcane industry by using the chemical and sequence analysis. These bacteria can be used for the laboratory and large scale production of different types of PHAs.
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ڈاکٹر ضیاء الدین

ڈاکٹر ضیاء الدین مرحوم
افسوس ہے کہ گزشتہ ۲۴؍ دسمبر ۱۹۴۰؁ء کو ڈاکٹر سر ضیاء الدین مرحوم نے بعارضہ فالج لندن میں انتقال کیا، مرحوم اپنے علمی کمال میں ہندوستان کے مشاہیر میں تھے، وہ تعلیم کے ماہر اور ریاضیات کے ممتاز فاضل تھے، انھوں نے اپنی تعلیم کے زمانہ میں ان فنون میں ہندوستان اور یورپ کی درسگاہوں میں جو امتیازات حاصل کئے، وہ اس دور میں کم ہندوستانیوں کو حاصل ہوئے تھے، چند دنوں جامعہ ازہر میں بھی رہے تھے، اس لئے عربی سے بھی کچھ واقف تھے، ان کی پوری زندگی علم و تعلیم کی خدمت میں گزری اور وہ نصف صدی سے زیادہ کسی نہ کسی حیثیت سے مدرسۃ العلوم اور اس کے بعد مسلم یونیورسٹی سے وابستہ رہے، کئی مرتبہ وائس چانسلر ہوئے، ابھی تھوڑے دن ہوئے کہ اس عہدہ سے سبکدوش ہوئے تھے، برسوں مجلس مقننہ کے ممبر رہے، مالیات میں ان کی رائے اور مشوروں کو خاص اہمیت حاصل تھی، ان کو اس زمانہ کے بڑے سے بڑے اعزاز حاصل ہوئے، جن سے یونیورسٹی کو بھی فوائد پہنچے اور ان کے دور میں اس کو بڑی ترقی ہوئی، ان کا آخری کارنامہ میڈیکل کالج کا قیام ہے، چند مہینے ہوئے جدید تعلیمی نظام کے مطالعہ کے لئے یورپ اور امریکہ گئے تھے، کہ لندن میں پیام اجل آپہنچا۔
مرحوم اس دور کی پیداوار تھے، جب مسلمانوں پر مغربی تمدن مسلط تھا، اور اس کا سب سے بڑا مرکز علی گڑھ تھا، لیکن انھوں نے اس کا بہت کم اثر قبول کیا، اب تو ضعیفی کی عمر تھی، وہ ہر زمانہ میں نہ صرف عقیدے بلکہ ظاہری وضع قطع میں بھی مسلمان رہے، ان کی زندگی بڑی سادہ اور بے تکلف تھی، ان کی سادگی میں ایک صاحبِ کمال کی شانِ بے نیازی پائی جاتی تھی، شخصی طور پر بھی ان...

Status of Women in Islam and its Impact on Pashtun Society in Present Times

This article discusses women rights in Islam and its impacts on Pashtoon society in the present times. The researcher talks about Islamic laws, history and the future of women rights in Islam in general and in Pashtoon society in particular. Islam is natural religion. As compared to other religions, Islam has emphasized on women rights, protection, dignity and their rights have always been focused. Islam has given a distinctive role to woman as a mother, as a sister, as daughter, and as a wife, and in other relations. According to Islamic injections, women are regarded so highly that it is said that paradise live under the feet of mother. A good wife is a great wealth of the world. Daughter is blessing of Allah. Islam has guaranteed women with education, training and all basic needs along with all types of protection. In the same manner, if we talk an over vice of Pashtoon society women rights holds great importance in the light of Islamic laws, Islamic code of conduct, customs and worship etc.

Development of Serodiagnostic Test for Tuberculosis by Using Mycobacterium Tuberculosis Specific Antigens

The effective removal and control of tuberculosis (TB) disease can be achieved with early and accurate diagnosis. It accounts for a majority of deaths and loss of health status thus damaging the economy. The present diagnostics for TB are not very effective as their sensitivity and specificity are low. Therefore the tests with more diagnostic value need to be developed. Thus a study was planned to develop an indigenous technology by exploiting biotechnology tools, and a new emerging technique i.e. multiplex microbead immunoassay (MMIA). Six potential recombinant antigenic genes: ag85a, ag85b, ag85c, cfp-10, esat-6 and hspx of Mycobacterium tuberculosis (M. tuberculosis) were selected for this purpose and respective genes were transformed into expression strain Bl21DE3pLysS for overexpression of proteins. Expression of each antigen was optimized for various conditions like concentration of isopropyl beta-D-1-thiogalactopyranoside (IPTG), time and temperature. Expression of protein was then confirmed by Western blotting. After confirmation, proteins were overexpressed in bulk cultures and purified by using immobilized metal affinity chromatography (IMAC) by using Histidine-tag (His-tag). The purified proteins were quantified and used to coat on microbeads at different concentrations and were used for analysis of collected blood samples. The blood samples of TB patients and healthy controls were collected from Federal TB Centre, Rawalpindi, from the students of Pir Mehr Ali Shah - Arid Agriculture University Rawalpindi (PMAS-AAUR), Rawalpindi, Punjab, Pakistan and healthy controls from USA. The collected human blood samples were divided as tuberculin skin test negative (TST -ve) healthy controls, from Pakistan and USA (group 1), TST positive (group 2), reactivated TB patients xx(group 3) and time points of active TB patients who were diagnosed and were under treatment (group 4). The coated microbeads were then used to analyze the presence of antibodies against M. tuberculosis in the collected blood samples. The results of group 1, Pakistan and USA group (TST negative) showed in general the absence of antibodies against any of the six antigens used in the MMIA. In the group 2 (TST positive), low median fluorescence intensity (MFI) values were detected against all antigens. Further in group 3 (reactivated TB patients) highest MFI values were observed against all antigens whereas in group 4 (active TB patient time points) MFI values were higher than group 2 but lower than group 3. This shows MMIA is very specific in detection of TB. Therefore, based on this it may be concluded that these antigens can be used to develop MMIA. However, use of more antigens and standardization is required.